This document investigates the role of O-GlcNAcylation in porcine satellite cell function. It finds that O-GlcNAcylation is not required for proliferation or early differentiation of satellite cells. However, excessive O-GlcNAcylation impairs late stage differentiation and myogenesis by repressing myogenin expression. It also inhibits regenerative myogenesis in satellite cells.

1. What is Role of O-GlcNAcylation in
Porcine SC Function?

2. MuSC On Myofibers

3. • O-GlcNAc
• TT04: OGT
inhibitor
• ThG: OGA
inhibitor

4. Proliferation

5. O-GlcNAcylation is dispensable for porcine
MuSC proliferation
0
5
10
15
20
25
30
Vehicle TT04 ThG
%BrdU+nuclei
P = 0.180
P = 0.094

6. Differentiation

7. O-GlcNAcylation has no effect on early
stage of muscle differentiation
P = 0.394
0
1
2
3
4
5
6
Vehicle TT04 ThG
FusionIndex(%)
0
2
4
6
8
10
12
Vehicle TT04 ThG
Fusionindex(%)
P = 0.196
P = 0.652P = 0.820
Day 1 Day 2

8. Excessive O-GlcNAcylation impairs late stage of
muscle differentiation (DM5)
Vehicle TT04
ThG
200 μm

9. Excessive O-GlcNAcylation impairs late stage of
muscle differentiation (DM5)
0
10
20
30
40
50
Vehicle TT04 ThG
Fusionindex(%)
P = 0.458
P < 0.001

10. Myogenin:
Differentiation Marker

11. 0
10
20
30
40
Vehicle TT04 ThG
%Myogenin+nuclei
P = 0.334
P < 0.001
Hyper-O-GlcNAcylation Impairs Myogenesis by
Repressing Myogenin Expression

12. Hyper-O-GlcNAcylation inhibits regenerative
myogenesis

13. Hyper-O-GlcNAcylation inhibits regenerative
myogenesis
0
50
100
150
200
250
300
ND Vehicle TT04 ThG
Diameter(μm)
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