1. Problems with Salmonella and Listeria in Food Products?
Visit us online at
foodcheksystems.com
Actero™ Enrichment Media is the SOLUTION!
To purchase our products, contact FoodChek™ distributor 1.800.932.5000 vwr.com

2. Protecting Your Customers, Their Families and Children from
Infection
According to the Centers for Disease Control and Prevention (CDC),
Salmonella causes about 1.4 million illnesses in the United States
every year, with about 23,000 hospitalizations and 450 deaths.
Concerning Listeria, approximately 1600 illnesses and 260 deaths due
to listeriosis occur annually in the United States. These statistics show
the importance for food processors to implement a thorough
pathogen surveillance program to protect human life.
Reduce the Risk of Salmonella or Listeria Contamination in
Food Products
The best way to prevent Salmonella infection is to always cook meat
and eggs to the suggested temperatures and be careful not to
contaminate other foods with the juices from uncooked meat, poultry
or eggs. Listeria infection can be prevented by thoroughly cooking all
meats (even lunchmeat, especially if you are pregnant), avoiding raw
or unpasteurized milk, cheese or juice, washing vegetables before
eating and cleaning hands, knives and cutting boards after dealing
with raw meat.
Actero™ Enrichment Media is the Solution for You
Due to sub-lethally injured pathogens cells surviving the
processing treatment of foods, an optimized method of recovery
and enrichment of the bacterium is needed. The Actero™
Enrichment Media provides for effective recovery of injured an/or
dormant pathogens cells from different food and environmental
samples after a single-step enrichment.
The Advantages of Actero™ Enrichment Media
Protect your brand, your customers and their families
Results in an amazing 14 to 30 hours* of enrichment time
Uses less enrichment media per volume compared to standard
enrichment media
Release your products in one day shift*
AOAC validated for a variety of food
Detect using a Real-Time PCR System or by the Actero™ culture
method
* Depending on the food matrix and its complexities
Visit us online at foodcheksystems.com
Foodborne pathogens can be found in
various foods. “Time-to-Results” and
accuracy are critical factors for food
processors being able to provide a safe
food supply, prevent foodborne diseases,
and protecting the brand and customers.

3. Actero™ Enrichment Media
is the ONLY solution for You
FoodChek’s ActeroTM
Enrichment Media
has received
AOAC PTM CERTIFICATION
on Real-Time PCR Assay and
Lateral Flow System
Actero™
Enrichment Media
is the proven solution
for problem matrices!
Protect your brand, your customers, their
families, and especially their children
Results in an amazing
one day shift
Achieve real cost reduction by converting to
Actero™ Enrichment Media

4. foodcheksystems.com | 1.877.298.0208
THE PROBLEM WITH SALMONELLA & LISTERIA
IN FOOD PRODUCTS
Hence, a variety of methods have been developed for rapid detection of foodborne
pathogens as it is required in many food analyses. Most of them require an enrichment step
before being able to apply the detection device.
For all these reasons, FoodChek has developed Actero™ Salmonella Enrichment Media and
Actero™ Listeria Enrichment Media to selectively enrich Salmonella and Listeria in a variety
of foods for its further detection. FoodChek understands the challenging regulatory and
business demands on food producers along with the importance of volume testing and
inventory release that protects your bottom line, without compromising product safety or
the integrity of your brand.
Early and sensitive detection of these foodborne pathogens remains a critical issue in public
health policy. The method to enrich and detect Salmonella and Listeria using Actero™
Enrichment Media was developed with input from the food industry to offer the best
practical
Salmonella spp. (species) is the leading cause of food infection in the United States, with an
estimated number of Salmonella infections of 1.4 million cases per year, and causes the
second largest number of human food-borne illnesses in Europe. Listeriosis caused by
Listeria monocytogenes (L. mono) is low in incidence but its mortality rate is high placing
the microorganism to the top of pathogens contributing to food-borne illness and resulting
in death. Older adults, pregnant women and their fetuses, newborn infants, and other
persons with reduced immune function are particularly at risk for invasive listeriosis.
Salmonella infections have been associated with eating foods, such as meat, eggs and fresh
produce contaminated with animal or human feces. The main causes of Salmonella illness
are poultry and eggs. Listeria monocytogenes has the potential to be present in all raw
foods. Cooked foods can also be contaminated and is usually as the result of post-process
contamination. The pathogen has been isolated from a very wide range of processed foods
includingincluding pâtés, milk, soft cheeses, ice cream, ready-to-eat cooked and fermented meats, smoked and lightly processed fish products and
other seafood.
It is essential to analyze the food for the presence of foodborne pathogens in order to ensure a safe food supply and to minimize the
occurrence of foodborne diseases. The conventional methods used to detect foodborne pathogens are time consuming and laborious.
Hence
With fast, reliable, and easily implemented testing workflows,
FoodChek can help you:
Minimize your risk
Maximize your competitive edge
Reduce your production costs
Protect your brand, your customers and their families
practical solution for a monitoring and surveillance program for these two pathogens.
Despite the inhibitory challenges associated with this sample matrix, our Actero™ Enrichment Media has AOAC-RI CERTIFICATION and is
approved on a variety of food and environmental testing solutions that are designed to accurately screen for Salmonella and Listeria,
with exceptional specificity and speed, helping to reduce costly false positives and inventory holds.

5. foodcheksystems.com | 1.877.298.0208
Protecting your brand, your customers;
their families and, especially their children,
depend on it.
The FoodChek Team specializes in food microbiology
that helps labs and processors to achieve optimum
efficiency in controlling pathogens throughout the
food processing chain.
The dedicated team of FoodChek professionals
have the expertise in facilitating the adoption of
Actero™ Enrichment Media in your plant by
providing you support in every step of the
conversion process.
As your food safety partner, the FoodChek
team assists you in implementing the best
possible approach for the control and the
monitoring of Salmonella and Listeria in
your food production.
FoodChek understands the importance of
achieving rapid “Time-to-Results” without
compromising accuracy and safety.
The FoodChek Team is well positioned to provide
customized service solutions designed for your specific
requirements.
IT’S EASY TO CONVERT TO
ACTERO™ ENRICHMENT MEDIA

6. foodcheksystems.com | 1.877.298.0208
Abstract
FoodChek Systems Inc., in collaboration with a Real-Time PCR Assay manufacturer, has developed effective methods for easy and rapid
detection of Salmonella and Listeria in a variety of environmental and food samples. The methods has been successfully granted AOAC
PTM CERTIFICATION. The results of the study demonstrated that performance of the candidate methods is statistically equivalent to the
reference culture method for detecting Salmonella or Listeria in food. The time to detect Salmonella spp. has been reduced up to several
hours, depending the matrix, including the enrichment and detection steps when the Actero™ Salmonella Enrichment Media was used.
Similar observation was obtained for the detection of Listeria in food using Actero™ Listeria Enrichment Media. No false negative or false
positive results were observed. The high accuracy and reliability of the methods were confirmed in the independent laboratory
validation study.
Materials for Salmonella studies
Actero™ Salmonella Enrichment Media
Dehydrated format:
• FCM-009 (VWR 97068-832)
• 500 g bottle and three supplement vials
Ready to use liquid formats:
• FCM-047 (VWR 89522-710) – 5 L MediaBox
• FCM-048 (VWR 89522-712) – 10 L MediaBox (with
supplements added)
API 20 E kits for identification of enterobacteriacae
Brilliant Green dye 1% solution
METHOD COMPARISON STUDY REPORT
EASY & RAPID DETECTION OF SALMONELLA AND LISTERIA IN FOOD
Real-Time PCR Assay Salmonella, Kit #D14306040*
Hektoen Enteric (HE) agar
Lactose broth
Rappaport-Vassiliadis (RV) broth
Salmonella serogroup E antiserum
Tetrathionate broth (TT)
Trypticase Soy agar plates with 5% of sheep blood agar
Triton X-100
Xylose Lysine Deoxycholate (XLD) agar
* Contact VWR for further details
Materials for Listeria studies
Actero™ Listeria Enrichment Media
Dehydrated formats:
• FCM-011 (VWR 97068-830) – 500 g
• FCM-022 (VWR 10158-484) – 2 kg
• FCM-192 (VWR 10146-992) – 1 L MediaPouch
• FCM-193 (VWR 10146-994) – 10 L MediaPouch
Ready to use liquid formats:
• FCM-045 (VWR 89522-706) – 5 L MediaBox
• FCM-046 (VWR 89522-708) – 10 L MediaBox
Real-Time PCR Assay Genus Listeria Kit #D15131113*
Buffered Listeria enrichment broth (BLEB)
UVM enrichment broth
MOPS – Buffered Listeria enrichment broth (MOPS-BLEB)
Modified Oxoid (MOX) agar plates
Trypticase Soy broth with 0.6% yeast extract (TSA-YE)
Horse blood overlay agar (HL)
Biochemical test panel Listeria API® system

7. foodcheksystems.com | 1.877.298.0208
Method for Validation Studies for Detection of Salmonella spp.
METHOD COMPARISON STUDY REPORT
EASY & RAPID DETECTION OF SALMONELLA AND LISTERIA IN FOOD
Method for Validation Studies for Detection of Listeria spp. & L. mono
Matrices that are AOAC RI-PTM validated (license number 041403) Matrices Internally Validated by FoodChek Laboratories
Food contact surface –
stainless steel and plastic
Dry pet food
Milk chocolate
Chocolate liquor
Cocoa powder
Shell eggs
Ground beef
Ground chicken
Chicken carcass rinse
Whole egg powder
Wheat flour
Peanut butter
Raw almonds
Dry raisins
Pasteurized liquid egg
Matrices that are AOAC RI-PTM validated (license number 111201) Matrices Internally Validated by FoodChek Laboratories
Food contact surface –
stainless steel and plastic
No food contact surface –
sealed concrete
Hot dog sausage
Cold smoked salmon
Frozen cooked shrimp
Soft Mexican style cheese
Fresh bagged spinach
Smoked turkey breast
Cured ham
Ice cream
Pasteurized milk
Raw fish

8. foodcheksystems.com | 1.877.298.0208
Results
Probability of detection (POD) statistical model was used to evaluate the differences between presumptive and confirmed results as well
as between the alternative and the reference methods.
The results of the different method comparison validation studies for variety of food products are summarized in the table below. POD
analysis didn’t show any significant differences (the 95% confidence interval of the dPODs contains zero) in the method performance
between the Real-Time PCR Assay and the reference methods.
Conclusions
The results of the study demonstrated that performance of the Real-Time PCR Assay method to detect Salmonella in a variety of food
samples enriched with Actero™ Salmonella Enrichment Media is equivalent to the reference method. It has been proven that Actero™
Salmonella Enrichment Media is able to selectively enrich Salmonella for its further detection in the shortest possible time.
METHOD COMPARISON STUDY REPORT
EASY & RAPID DETECTION OF SALMONELLA IN FOOD PRODUCTS
Matrix
CFU/Test
Portion
N
Real-Time PCR Method
US FDA BAM Chapter 5
Method dPOD
Confirmed POD Confirmed POD
Stainless Steel
0.0 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)
50.0 20 12 0.60 12 0.60 0.00 (-0.28; 0.28)
437.0 5 5 1.00 5 1.00 0.00 (-0.43; 0.43)
Plastic
0.0 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)
59.4 20 10 0.50 14 0.70 -0.20 (-0.45; 0.10)
400.0 5 5 1.00 5 1.00 0.00 (-0.43; 0.43)
Milk chocolate
˂0.075 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)
0.5 20 10 0.50 9 0.45 0.05 (-0.24; 0.33)
8.7 5 5 1.00 5 1.00 0.00 (-0.43; 0.43)
Chocolate liquor
˂0.075 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)
0.1 20 9 0.45 2 0.10 0.35 (0.07; 0.57)
2.7 5 5 1.00 5 1.00 0.00 (-0.28; 0.28)
Cocoa powder
˂0.075 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)
0.6 20 10 0.50 9 0.45 0.05 (-0.24; 0.33)
2.0 5 3 0.60 5 1.00 -0.40 (-0.77; 0.12)
Shell egg
˂0.075 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)
0.3 20 7 0.35 6 0.30 0.05 (-0.23; 0.32)
1.2 5 4 0.80 4 0.80 0.00 (-0.46; 0.46)
Dry pet food
(25 g)
˂0.075 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)
1.1 20 13 0.65 16 0.80 -0.15 (-0.40; 0.12)
3.7 5 5 1.00 5 1.00 0.00 (-0.43; 0.43)
Dry pet food
(375 g)
˂0.075 5 0 0.00 0 0.00 0.00 (-0.43; 0.43)
1.5 20 14 0.70 15 0.75 -0.05 (-0.31; 0.22)
8.7 5 5 1.00 5 1.00 0.00 (-0.43; 0.43)

9. foodcheksystems.com | 1.877.298.0208
Results
Probability of detection (POD) statistical model was used to evaluate the differences between presumptive and confirmed results as well
as between the alternative and the reference methods. The validation was done for the detection of Listeria spp. and L. monocytogenes,
however, only the results for the detection of Listeria spp. are shown.
The results of the different method comparison validation studies for variety of food products are summarized in the table below. POD
analysis didn’t show any significant differences (the 95% confidence interval of the dPODs contains zero) in the method performance
between the Real-Time PCR Assay and the reference methods.
Conclusions
The results of the study demonstrated that performance of the Real-Time PCR Assay method to detect Listeria in a variety of food
samples enriched with Actero™ Listeria Enrichment Media is equivalent to the reference method. It has been proven that Actero™
Listeria Enrichment Media is able to selectively enrich Listeria for its further detection in the shortest possible time.
METHOD COMPARISON STUDY REPORT
EASY & RAPID DETECTION OF LISTERIA IN FOOD PRODUCTS
Matrix
CFU/Test
Portion
N
Real-Time PCR Method
US FDA BAM Chapter 5
Method dPOD
Confirmed POD Confirmed POD
Stainless steel
0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)
90.0 20 8 0.40 5 0.25 0.15 (-0.13, 0.40)
220.0 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)
Plastic
0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)
4.8 20 10 0.50 5 0.25 0.25 (-0.05, 0.49)
58.0 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)
Sealed concrete
0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)
4.3 20 11 0.55 4 0.20 0.35 (0.05, 0.58)
22.0 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)
Frankfurters
0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)
0.3 20 14 0.70 7 0.35 0.35 (0.04, 0.58)
3.9 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)
Fresh bagged
spinach
0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)
1.6 20 16 0.80 15 0.75 0.05 (-0.21, 0.30)
1000.0 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)
Soft Mexican style
cheese
0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)
0.6 20 10 0.50 8 0.40 0.10 (-0.19, 0.37)
3.6 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)
Frozen cooked
shrimp
0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)
0.7 20 12 0.60 11 0.55 0.05 (-0.24, 0.33)
5.3 5 5 1.00 5 1.00 0.00 (-0.43, 0.43)
Cold smoked salmon
0.0 5 0 0.00 0 0.00 0.00 (-0.43, 0.43)
1.3 20 14 0.70 14 0.70 0.00 (-0.27, 0.27)
13.9 5 5 1.00 5 1.0 0.00 (-0.43, 0.43)

10. ACCELERATE YOUR REAL-TIME PCR
ASSAY RESULTS WITH ACTERO™
SALMONELLA ENRICHMENT MEDIA
Enrichment of Salmonella spp. in Actero™ Salmonella vs other media prior to Real-Time PCR Assay.
Actero™ Salmonella Enrichment Media
Actero™ Salmonella Enrichment Media is a selective media optimized for a rapid single step enrichment of Salmonella from food and environmental samples
FoodChek PN VWRPN Package Size Product Format
FCM-009 97068-836 500g Dehydrated powders and three
supplement vials
FCM-047 89522-710 MediaBox™ 5L
MediaBox™
Ready to use liquidFCM-048 89522-712 MediaBox™ 10L
To request a sample, please visit vwr.com or call 1.800.932.5000
Matrix
ActeroTM Salmonella
Method
Media A Method
Validation
Type Size Time
Medium
volume
Time
Medium
volume
Stainless steel 100 cm2 14 – 18 h 90 mL 18–24 h 225 mL
AOAC-PTM
validated
Plastic 100 cm2 14 – 18 h 90 mL 18–24 h 225 mL
Milk chocolate 25 g 22 – 26 h 175 mL Not validated
Chocolate liquor 25 g 26 – 30 h 225 mL Not validated
Cocoa powder 25 g 16 – 20 h 175 mL 22 – 26 h 225 mL
Dry pet food 25 g 18–22 h 225 mL Not validated
Dry pet food 375 g 18–22 h 2625 mL 22–26 h 3375 mL
Shell eggs 20 eggs 16 – 20 h 1000 mL 48 h 2000 mL
Ground beef 25 g 16 – 20 h 75 mL 20 – 24 h 225 mL
Internally
validated
Ground beef 375 g 20 – 24 h 1125 mL 22 – 26 h 1500 mL
Ground chicken 25 g 14 – 18 h 225 mL 16 – 24 h 225 mL
Chicken carcass rinse 1 carcass 16 – 20 h 30 mL 22 – 26 h 30 mL
Whole egg powder 100 g 14 – 18 h 600 mL 22 – 26 h 225 mL
Pasteurized liquid egg 100 g 18 – 22 h 100 mL Not validated
Peanut butter 25 g 16 – 20 h 175 mL 22 – 26 h 225 mL
Raw almonds 375 g 16 – 20 h 750 mL Not validated
Dry raisins 25 g 16 – 20 h 75 mL Not validated
Wheat flour 25 g 18 – 22 h 175 mL Not validated

11. ACCELERATE YOUR REAL-TIME PCR
ASSAY RESULTS WITH ACTERO™
LISTERIA ENRICHMENT MEDIA
Enrichment of Listeria spp. and L. monocytogenes in Actero™ Listeria vs other media prior to Real-Time PCR
Assay.
Matrix
ActeroTM Listeria
Method
Media A Method
Validation
Type Size Time
Medium
volume
Time Medium volume
Stainless steel 100 cm2 20 – 24 h 90 mL 24 - 48 h 90 mL
AOAC-PTM
validated
Plastic 100 cm2 20 – 24 h 90 mL 18–24 h 90 mL
Sealed concrete 100 cm2 20 – 24 h 90 mL Not validated
Hot dog sausage 125 g 26 – 30 h 750 mL 28 – 48 h 500 mL
Cold smoked salmon 25 g 22 – 24 h 150 mL Not validated
Frozen cooked shrimp 25 g 22 – 24 h 150 mL 24 - 48 h 225mL
Soft Mexican style cheese 25 g 22 – 24 h 150 mL 26 – 48 h 225 mL
Fresh bagged spinach 25 g 22 – 24 h 150 mL 24 – 48 h 225 mL
Smoked turkey breast 125 g 16 – 20 h 500 mL Not validated
Internally
validated
Cured ham 125 g 26 – 30 h 500 mL Not validated
Ice cream 25 g 22 – 24 h 150 mL Not validated
Pasteurized milk 25 g (mL) 22 – 24 h 150 mL Not validated
Raw fish 25 g 18 – 22 h 100 mL Not validated
Actero™ Listeria Enrichment Media
Actero™ Listeria Enrichment Media is a selective media optimized for a rapid single step enrichment of Listeria from food and environmental samples
FoodChek PN VWRPN Package Size Product Format
FCM-011 97068-830 500 g
Dehydrated powders
FCM-022 10146-990 2 kg
FCM-045 89522-706 MediaBox™ 5L
MediaBox™
Ready to use liquidFCM-046 89522-708 MediaBox™ 10L
To request a sample, please visit vwr.com or call 1.800.932.5000

12. FoodChek Systems Inc.
450, 1414 – 8 Street SW, Calgary, Alberta, T2R 1J6, Canada
Terry Macartney
Executive Vice President, Operations and Product
Development
North America toll free: 1.877.298.0208 x4
Tel: 403.269.9424 x4
Email: tmacartney@foodcheksystems.com
Carlos Ruiz
Vice President, Strategic Business Development
Cell: 678.648.6121
Email: cruiz@foodcheksystems.com
Carl (Chip) Zerr III
Vice President, Product Integration
Tel: 856.839.4213
Cell: 856.200.9184
Email: czerr@foodcheksystems.com
David Claveau, M.Sc, M.B.A
Senior Product Manager
North America toll free: 1.877.298.0208 x2
Tel: 403.269.9424 x2
Email: dclaveau@foodcheksystems.com
Problems with Salmonella and Listeria in Food Products?
Actero™ Enrichment Media is the SOLUTION!