Affinity chromatography as per PCI

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preetimanepatil

The presentation covers the principle, operation technique and applications of affinity chromatography

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HotJupiters are amongthebest-studied exoplanets, but it is still poorly understood how their chemical composition and cloud properties vary with longitude. Theoretical models predict that clouds may condense on the nightside and that molecular abundances can be driven out of equilibrium by zonal winds. Here we report a phase-resolved emission spectrum of the hot Jupiter WASP-43b measured from 5–12µ 5–12µ 5–12µm with JWST’s Mid-Infrared Instrument (MIRI). 1524 ±35 1524 ±35 and 863±23 The spectra reveal a large day–night temperature contrast (with average brightness temperatures of 1524 ± 35 863 ±23 863 ±23Kelvin, respectively) and evidence for water absorption at all orbital phases. Comparisons with three-dimensional atmospheric models show that both the phase curve shape and emission spectra strongly suggest the presence of nightside clouds which become optically thick to thermal emission at pressures greater than ∼100mbar. The dayside is consistent with a cloudless atmosphere above the mid-infrared photosphere. Con3trary to expectations from equilibrium chemistry but consistent with disequilibrium kinetics models, methane is not detected on the nightside (2σ upper limit of 1–6 parts per million, depending on model assumptions).

Nightside clouds and disequilibrium chemistry on the hot Jupiter WASP-43b

Sérgio Sacani

Botany is the branch of biology that deals with the scientific study of plants, including their structure, growth, reproduction, metabolism, development, and classification. It encompasses a wide range of topics, from the molecular biology of plant cells to the ecological relationships between plants and their environments. Botany is essential for understanding plant diversity, ecology, evolution, and the roles plants play in ecosystems and human societies.

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kantirani197

We explore different scenarios to explain the chemical difference found in the remarkable giant-giant binary system HD138202+CD−3012303. For the first time, we suggest how to distinguish these scenarios by taking advantage of the extensive convective envelopes of giant stars. Methods. We carried out a high-precision determination of stellar parameters and abundances by applying a full line-by-line differential analysis on GHOST high-resolution spectra. We used the FUNDPAR program with ATLAS12 model atmospheres and specific opacities calculated for an arbitrary composition through a doubly iterated method. Physical parameters were estimated with the isochrones package and evolutionary tracks were calculated via MIST models. Results. We found a significant chemical difference between the two stars (∆[Fe/H]∼0.08dex), which is largely unexpected considering the insensitivity of giant stars to planetary ingestion and diffusion effects. We tested the possibility of engulfment events by using several different combinations of stellar mass, ingested mass, metallicity of the engulfed object and different convective envelopes. However, the planetary ingestion scenario does not seem to explain the observed differences. For the first time, we distinguished the source of chemical differences using a giant-giant binary system. By ruling out other possible scenarios such as planet formation and evolutionary effects between the two stars, we suggest that primordial inhomogeneities might explain the observed differences. This remarkable result implies that the metallicity differences that were observed in at least some main-sequence binary systems might be related to primordial inhomogeneities rather than engulfment events. We also discuss the important implications of finding primordial inhomogeneities, which affect chemical tagging and other fields such as planet formation. We strongly encourage the use of giantgiant pairs. They are a relevant complement to main-sequence pairs for determining the origin of the observed chemical differences in multiple systems.

Disentangling the origin of chemical differences using GHOST

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Context. WASP-76 b has been a recurrent subject of study since the detection of a signature in high-resolution transit spectroscopy data indicating an asymmetry between the two limbs of the planet. The existence of this asymmetric signature has been confirmed by multiple studies, but its physical origin is still under debate. In addition, it contrasts with the absence of asymmetry reported in the infrared (IR) phase curve. Aims. We provide a more comprehensive dataset of WASP-76 b with the goal of drawing a complete view of the physical processes at work in this atmosphere. In particular, we attempt to reconcile visible high-resolution transit spectroscopy data and IR broadband phase curves. Methods. We gathered 3 phase curves, 20 occultations, and 6 transits for WASP-76 b in the visible with the CHEOPS space telescope. We also report the analysis of three unpublished sectors observed by the TESS space telescope (also in the visible), which represents 34 phase curves. Results. WASP-76 b displays an occultation of 260±11 and 152±10 ppm in TESS and CHEOPS bandpasses respectively. Depending on the composition assumed for the atmosphere and the data reduction used for the IR data, we derived geometric albedo estimates that range from 0.05 ± 0.023 to 0.146 ± 0.013 and from <0.13 to 0.189 ± 0.017 in the CHEOPS and TESS bandpasses, respectively. As expected from the IR phase curves, a low-order model of the phase curves does not yield any detectable asymmetry in the visible either. However, an empirical model allowing for sharper phase curve variations offers a hint of a flux excess before the occultation, with an amplitude of ∼40 ppm, an orbital offset of ∼−30◦ , and a width of ∼20◦ . We also constrained the orbital eccentricity of WASP-76 b to a value lower than 0.0067, with a 99.7% confidence level. This result contradicts earlier proposed scenarios aimed at explaining the asymmetry observed in high-resolution transit spectroscopy. Conclusions. In light of these findings, we hypothesise that WASP-76 b could have night-side clouds that extend predominantly towards its eastern limb. At this limb, the clouds would be associated with spherical droplets or spherically shaped aerosols of an unknown species, which would be responsible for a glory effect in the visible phase curves.

Asymmetry in the atmosphere of the ultra-hot Jupiter WASP-76 b

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Zoology 4th semester series (krishna).pdf

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Bollworms are among the most damaging pests in cotton cultivation, affecting the bolls where the cotton fibers are formed. There are several species of bollworms, each capable of causing significant yield loss and quality degradation if not effectively managed. Here’s a detailed look at the primary bollworm species affecting cotton: Cotton Bollworm (Helicoverpa armigera): Also known as the corn earworm or the Old World bollworm, this pest is found in many regions around the world. It is highly polyphagous (feeds on many different plants) and poses a threat not only to cotton but also to maize, tomatoes, and legumes. The larvae bore into the cotton bolls, feeding on the developing seeds and fibers, which can lead to boll rot. Pink Bollworm (Pectinophora gossypiella): A significant pest of cotton, the pink bollworm larvae infest the cotton bolls, feeding on the seeds and lint. This can severely damage or destroy the bolls. In regions where pink bollworms are prevalent, they have been a major driver for the adoption of genetically engineered Bt cotton, which expresses a bacterium gene toxic to certain insects. Tobacco Budworm (Heliothis virescens): Closely related to the cotton bollworm, the tobacco budworm primarily attacks tobacco but is also a common pest in cotton. It primarily damages the flowers and bolls of the cotton plant. Differentiating between the tobacco budworm and the cotton bollworm based on appearance can be challenging, but it is crucial for effective management. American Bollworm (Helicoverpa zea): Known in some regions as the corn earworm, it is similar in behavior to Helicoverpa armigera and poses a threat to a variety of crops, including cotton. The larvae attack the cotton bolls, leading to direct damage to the cotton lint and seeds. Management Strategies: Cultural Controls: Crop rotation, destruction of crop residues, and deep plowing can help break the pest’s life cycle. Timing of planting can also be adjusted to avoid peak pest infestation. Biological Controls: Natural enemies like Trichogramma wasps, which parasitize bollworm eggs, and predators such as lacewings and ladybugs can be encouraged. Bacillus thuringiensis (Bt) products can also be sprayed, which are particularly effective against young larvae. Chemical Controls: Insecticides may be required when infestation levels exceed economic thresholds. However, resistance management must be considered, alternating modes of action to avoid developing resistance. Genetic Approaches: Bt cotton, genetically modified to express Bacillus thuringiensis toxin, has been highly effective in controlling bollworms and has dramatically reduced the reliance on chemical insecticides. Monitoring and Scouting: Regular field scouting and using pheromone traps to monitor adult populations can help in timely and targeted application of control measures. The effective management of bollworms often requires an integrated approach

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Affinity chromatography as per PCI

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