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COMPARISON OF DIFFERENT SORBENTS FOR THE SIMULTANEOUS SPE OF EIGHT NSAIDs FROM HUMAN PLASMA COUPLED WITH HPLC. G. Carlucci , M. D’Ercole, L. Di Federico, P.Iuliani, M. Locatelli Dipartimento di Scienze del Farmaco-Facoltà di Farmacia-Università degli Studi “G. D’Annunzio” di Chieti-Pescara-Via dei Vestini 66100 Chieti-Italy. Introduction Sample preparation remains a central feature of pharmaceutical and biomedical analysis, and the time consuming nature of the current methodology has resulted in a continuous search for new and improved techniques. Solid phase extraction (SPE) is an important area with the introduction of new phases or formats and development of fully automated methods. The bulks of the commercially available phases based on bonded silicas (C18, C8, C4, etc.) have been very successful, whilst the presence of residual silanols often complicates the extraction of ionic compounds because of ionic interactions e.g. 1,3 . Recently a number of non-silica based polymeric SPE sorbents have been introduced which offer a potential for a different range of selectivities to silica based materials. In the present study we developed a SPE-HPLC procedure for the simultaneous analysis of seven widely used antiinflammatory drugs (NSAIDs), namely: furprofen, indoprofen, ketoprofen, fenbufen, flurbiprofen, indomethacin and ibuprofen ( Fig. 1 ). To achieve the most efficient preconcentration of the analytes from human plasma we investigated the retention ability of five commercially available SPE cartridges based on different kinds of sorbents, such as octadecyl-bonded silica (Sep-Pak, Waters), polymeric (Evolute, Argonaut and Bond Elute Plexa, Varian), divinylbenzene-N-vinylpyrrolidone copolimers (Oasis HLB, Waters) and styrene-divinylbenzene polymer (StrataX, Phenomenex). The identification and quantification of the investigated NSAIDs was carried out by means of HPLC coupled with diode array detection (DAD) at isocratic conditions. ,[object Object],[object Object],[object Object],Fig. 1 ,[object Object],[object Object],[object Object],Results and discussion For the development of SPE method and for the comparison of the different sorbents three concentrations levels were studied, taking into account of the normal plasmatic levels of NSAIDs in treated patients. The average extraction recoveries obtained from experiments carried out in triplicate are collected in Table 2 . Apparently the overall performance of the selected cartridges is not appreciably influenced by drug concentration. The best recoveries were provided by Oasis HLB, Bond-Elut and Strata X. Table 2 Comparison of retention ability of the five selected sorbents furprofen indoprofen ketoprofen fenbufen flurbiprofen indomethacin ibuprofen Fig. 2 Chromatogram of a standard solution of NSAIDs extracted @ 218 nm. Table 1 Analytical data for the HPLC method

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