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Màriam El Mnajed
RECONEIXEMENT DE LES PROTEÏNES AL LABORATORI:
MATERIAL NECESSARI:
- Tubs d'assaig i gradeta
- Pinces
- Bunsen o fogonet d'alcohol
- Vas de precipitats
- Àcid nítric concentrat
- Hidròxid sòdic al 40%
- Sulfat de coure a l'1%
- Albúmina d'ou, gelatina
PROCEDIMENT:
Vam començar amb la Reacció xantoproteica i el procediment va ser el
següent:
REACCIÓXANTOPROTEICA:
1- En un tub d'assaig vam posar 2ml de solució d'ovoalbúmina.
2- Vam afegir 1ml d'àcid nítric concentrat i el vam escalfar amb molt de compte
amb l'encenedor bunsen.
3- Vam apuntar els resultats. Vam posar el tub en un vas de precipitats ple
d'aigua a temperatura normal ja que ja era bastant freda.
4- Quan ja és va refredar bastant vam afegir 1ml de NaOH al 40% pero
nosaltres el vam passar a 8gr de NaOH i 12gr d'H2O perquè no necessitavem
tant material.
5- Vam anotar els canvis que vam observar.
6- Finalment vam tornar a fer tot aquest proçés però aquest cop, amb aigua.
Màriam El Mnajed
PROVA DELBIURET:
1- Vam començar aplicant 2ml de solució d'ovoalbúmina en un tub d'assaig.
2- Seguidament vam afegir 5ml de NaOH al 40% ( 8gr de NaOH i 12gr d'H2O)
3- Vam afegir unes gotes de sulfat del coure a l'1% ( 0,1gr de coure i 9,9gr
d'H2O)
4- Esperem, observem i anotem els resultats.
5- Finalment vam fer el mateix pero aquest cop amb aigua.

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Reconeixement de les proteïnes al laboratori

  • 1. Màriam El Mnajed RECONEIXEMENT DE LES PROTEÏNES AL LABORATORI: MATERIAL NECESSARI: - Tubs d'assaig i gradeta - Pinces - Bunsen o fogonet d'alcohol - Vas de precipitats - Àcid nítric concentrat - Hidròxid sòdic al 40% - Sulfat de coure a l'1% - Albúmina d'ou, gelatina PROCEDIMENT: Vam començar amb la Reacció xantoproteica i el procediment va ser el següent: REACCIÓXANTOPROTEICA: 1- En un tub d'assaig vam posar 2ml de solució d'ovoalbúmina. 2- Vam afegir 1ml d'àcid nítric concentrat i el vam escalfar amb molt de compte amb l'encenedor bunsen. 3- Vam apuntar els resultats. Vam posar el tub en un vas de precipitats ple d'aigua a temperatura normal ja que ja era bastant freda. 4- Quan ja és va refredar bastant vam afegir 1ml de NaOH al 40% pero nosaltres el vam passar a 8gr de NaOH i 12gr d'H2O perquè no necessitavem tant material. 5- Vam anotar els canvis que vam observar. 6- Finalment vam tornar a fer tot aquest proçés però aquest cop, amb aigua.
  • 2. Màriam El Mnajed PROVA DELBIURET: 1- Vam començar aplicant 2ml de solució d'ovoalbúmina en un tub d'assaig. 2- Seguidament vam afegir 5ml de NaOH al 40% ( 8gr de NaOH i 12gr d'H2O) 3- Vam afegir unes gotes de sulfat del coure a l'1% ( 0,1gr de coure i 9,9gr d'H2O) 4- Esperem, observem i anotem els resultats. 5- Finalment vam fer el mateix pero aquest cop amb aigua.