2. PrefacePreface
The following pictures are examples with descriptions of cytology slidesThe following pictures are examples with descriptions of cytology slides
processed with theprocessed with the Liqui-PREPLiqui-PREPTMTM
SystemSystem. The descriptions are reviewed. The descriptions are reviewed
by Pathologists. It will be noticed that descriptions of similar pictures areby Pathologists. It will be noticed that descriptions of similar pictures are
duplicated. The different pictures for a giving description are to show theduplicated. The different pictures for a giving description are to show the
observed consistency of actual theobserved consistency of actual the Liqui-PREPLiqui-PREPTMTM
produced slides.produced slides.
It should be noted, theIt should be noted, the Liqui-PREPLiqui-PREPTMTM
SystemSystem uses classical laboratoryuses classical laboratory
techniques to process a cytology specimen. The resulting slides are fromtechniques to process a cytology specimen. The resulting slides are from
randomized homogeneous aliquots of the cytology specimen. Once therandomized homogeneous aliquots of the cytology specimen. Once the
Liqui-PREPLiqui-PREPTMTM
processed slide is made and stained, diagnosis is performedprocessed slide is made and stained, diagnosis is performed
by trained professionals by reading the preparedby trained professionals by reading the prepared Liqui-PREPLiqui-PREPTMTM
slide.slide.
The specimens used for this atlas are from evaluation studies made by theThe specimens used for this atlas are from evaluation studies made by the
following sources:following sources:
LGM International, Inc. Studies and End User Quality AssuranceLGM International, Inc. Studies and End User Quality Assurance
program.program.
Selected Routine Specimen Pictures – N. Anontwatanawong, NationalSelected Routine Specimen Pictures – N. Anontwatanawong, National
Cancer Institute, Thailand.Cancer Institute, Thailand.
We want to thank the following professionals that have reviewed slides andWe want to thank the following professionals that have reviewed slides and
pictures, made the interpretations and/or reviewed this Atlas for technicalpictures, made the interpretations and/or reviewed this Atlas for technical
quality.quality.
Dr. Ivan Rapado, M.D. – Pathologist: Consulting Medical Director LGMDr. Ivan Rapado, M.D. – Pathologist: Consulting Medical Director LGM
International, Inc.International, Inc.
LGM International,
3. QUALIFICATIONQUALIFICATION
101-Endocervical Cells:101-Endocervical Cells:
40 x magnification picture of40 x magnification picture of
numerous columnar cells.numerous columnar cells.
Columnar cells with basallyColumnar cells with basally
located nuclei, vacuolatedlocated nuclei, vacuolated
cytoplasm and prominent cellcytoplasm and prominent cell
borders.borders.
LGM International,
102-Endocervical Cells:102-Endocervical Cells:
40 x magnification picture of40 x magnification picture of
two columnar cells.two columnar cells.
Columnar cells with basallyColumnar cells with basally
located nuclei, vacuolatedlocated nuclei, vacuolated
cytoplasm and prominent cellcytoplasm and prominent cell
borders.borders.
4. QUALIFICATIONQUALIFICATION
103-Endocervical Cells:103-Endocervical Cells:
40 x magnification picture of40 x magnification picture of
several columnar cells.several columnar cells.
Columnar cells with basallyColumnar cells with basally
located nuclei, vacuolatedlocated nuclei, vacuolated
cytoplasm and prominent cellcytoplasm and prominent cell
borders.borders.
LGM International,
104-Endocervical Cells:104-Endocervical Cells:
40 x magnification picture of40 x magnification picture of
numerous columnar cells,numerous columnar cells,
some isolated and a groupsome isolated and a group
arranged in aarranged in a
“honeycomb”configuration.“honeycomb”configuration.
5. QUALIFICATIONQUALIFICATION
107-Endocervical Cells:107-Endocervical Cells:
40 x magnification picture of40 x magnification picture of
columnar cells arranged incolumnar cells arranged in
a “honeycomb” configurationa “honeycomb” configuration
along with superficialalong with superficial
squamous cells.squamous cells.
The group of cells remainThe group of cells remain
attached at their basal plate.attached at their basal plate.
LGM International,
6. WITHIN NORMAL LIMITSWITHIN NORMAL LIMITS
109-Negative within Normal Limits:109-Negative within Normal Limits:
10x magnification picture shows a well10x magnification picture shows a well
dispersed population of superficialdispersed population of superficial
squamous cells. A few inflammatorysquamous cells. A few inflammatory
cells are present in the background.cells are present in the background.
The nuclei are round, small (pyknotic).The nuclei are round, small (pyknotic).
The chromatin are dense and theThe chromatin are dense and the
nucleoli are absent.nucleoli are absent.
The cytoplasm are polygonal,The cytoplasm are polygonal,
abundant, pale, clear (transparent) andabundant, pale, clear (transparent) and
eosinophilic The N/C ratio is 1:10.eosinophilic The N/C ratio is 1:10.
LGM International,
110-Negative within Normal Limits:110-Negative within Normal Limits:
10x magnification picture shows a well10x magnification picture shows a well
dispersed population of superficialdispersed population of superficial
squamous cells. A few inflammatorysquamous cells. A few inflammatory
cells are present in the background.cells are present in the background.
The nuclei are round, small (pyknotic).The nuclei are round, small (pyknotic).
The chromatin are dense and theThe chromatin are dense and the
nucleoli are absent.nucleoli are absent.
The cytoplasm are polygonal, abundant,The cytoplasm are polygonal, abundant,
pale, clear (transparent) andpale, clear (transparent) and
eosinophilic The N/C ratio is 1:10.eosinophilic The N/C ratio is 1:10.
7. WITHIN NORMAL LIMITSWITHIN NORMAL LIMITS
111-Negative within Normal Limits :111-Negative within Normal Limits :
40x magnification picture shows a well40x magnification picture shows a well
dispersed population of superficialdispersed population of superficial
squamous cells. A few inflammatory cellssquamous cells. A few inflammatory cells
are present in the background.are present in the background.
The nuclei are round, small (pyknotic).The nuclei are round, small (pyknotic).
The chromatin are dense and the nucleoliThe chromatin are dense and the nucleoli
are absent.are absent.
The cytoplasm are polygonal, abundant,The cytoplasm are polygonal, abundant,
pale, clear (transparent) and eosinophilicpale, clear (transparent) and eosinophilic
The N/C ratio is 1:10.The N/C ratio is 1:10.
112-Negative within Normal Limits :112-Negative within Normal Limits :
10x magnification picture shows a well10x magnification picture shows a well
dispersed population of superficialdispersed population of superficial
squamous cells. A few inflammatory cellssquamous cells. A few inflammatory cells
are present in the background.are present in the background.
The nuclei are round, small (pyknotic).The nuclei are round, small (pyknotic).
The chromatin are dense and the nucleoliThe chromatin are dense and the nucleoli
are absent.are absent.
The cytoplasm are polygonal, abundant,The cytoplasm are polygonal, abundant,
pale, clear (transparent) and eosinophilicpale, clear (transparent) and eosinophilic
The N/C ratio is 1:10.The N/C ratio is 1:10. LGM International,
8. WITHIN NORMAL LIMITSWITHIN NORMAL LIMITS
113-Negative within Normal Limits:113-Negative within Normal Limits:
10x magnification picture shows a well10x magnification picture shows a well
dispersed population of superficialdispersed population of superficial
squamous cells. A few inflammatory cellssquamous cells. A few inflammatory cells
are present in the background.are present in the background.
The nuclei are round, small (pyknotic).The nuclei are round, small (pyknotic).
The chromatin are dense and the nucleoliThe chromatin are dense and the nucleoli
are absent.are absent.
The cytoplasm are polygonal, abundant,The cytoplasm are polygonal, abundant,
pale, clear (transparent) and eosinophilicpale, clear (transparent) and eosinophilic
The N/C ratio is 1:10.The N/C ratio is 1:10.
114-Negative within Normal Limits:114-Negative within Normal Limits:
10x magnification picture shows a well10x magnification picture shows a well
dispersed population of superficialdispersed population of superficial
squamous cells. A few inflammatory cellssquamous cells. A few inflammatory cells
are present in the background.are present in the background.
The nuclei are round, small (pyknotic).The nuclei are round, small (pyknotic).
The chromatin are dense and the nucleoliThe chromatin are dense and the nucleoli
are absent.are absent.
The cytoplasm are polygonal, abundant,The cytoplasm are polygonal, abundant,
pale, clear (transparent) and eosinophilicpale, clear (transparent) and eosinophilic
The N/C ratio is 1:10.The N/C ratio is 1:10. LGM International,
9. BENIGN CELLULAR CHANGESBENIGN CELLULAR CHANGES
117-Cellular Changes due to Inflammation:117-Cellular Changes due to Inflammation:
40X magnification picture of superficial40X magnification picture of superficial
cells.cells.
A few inflammatory cells are coveringA few inflammatory cells are covering
the squamous cells.the squamous cells.
The nuclei are mildly enlarged due toThe nuclei are mildly enlarged due to
inflammation.inflammation.
118-Cellular Changes due to Inflammation:118-Cellular Changes due to Inflammation:
10X magnification picture of superficial,10X magnification picture of superficial,
intermediateintermediate (1)(1) and metaplastic cellsand metaplastic cells
(2)(2)..
Numerous inflammatory cells areNumerous inflammatory cells are
covering but not obscuring squamouscovering but not obscuring squamous
cells.cells.
The squamous cells show mild nuclearThe squamous cells show mild nuclear
enlargement.enlargement.
LGM International,
2 1
10. BENIGN CELLULAR CHANGESBENIGN CELLULAR CHANGES
119-Cellular Changes due to Inflammation:119-Cellular Changes due to Inflammation:
10X magnification picture of superficial10X magnification picture of superficial
cells.cells.
Numerous inflammatory cells areNumerous inflammatory cells are
covering but not obscuring squamouscovering but not obscuring squamous
cells.cells.
The nuclei are mildly enlarged due toThe nuclei are mildly enlarged due to
inflammation.inflammation.
120-Cellular Changes due to Inflammation:120-Cellular Changes due to Inflammation:
40X magnification of superficial,40X magnification of superficial,
intermediate cells and inflammatory cellsintermediate cells and inflammatory cells
covering but not obscuring the squamouscovering but not obscuring the squamous
cells.cells.
There is mild to moderate nuclearThere is mild to moderate nuclear
enlargement. Moderate enlargementenlargement. Moderate enlargement (1)(1)
The chromatin is smudgy and pale.The chromatin is smudgy and pale.
Micro nucleoli are seen and slight there isMicro nucleoli are seen and slight there is
perinuclear clearing.perinuclear clearing.
LGM International,
1
11. BENIGN CELLULAR CHANGESBENIGN CELLULAR CHANGES
127-Bacterial Flora Shift “Clue Cell”:127-Bacterial Flora Shift “Clue Cell”:
40X picture of intermediate squamous40X picture of intermediate squamous
cells covered by bacteria (“Clue Cell”).cells covered by bacteria (“Clue Cell”).
Consistent with Gardnerella vaginalis.Consistent with Gardnerella vaginalis.
128-Bacterial Flora Shift “Clue Cell”:128-Bacterial Flora Shift “Clue Cell”:
40X picture of intermediate squamous40X picture of intermediate squamous
cells covered by bacteria (“Clue Cell”).cells covered by bacteria (“Clue Cell”).
Consistent with Gardnerella vaginalis.Consistent with Gardnerella vaginalis.
LGM International,
12. BENIGN CELLULAR CHANGESBENIGN CELLULAR CHANGES
129-Cellular Changes associated with Herpes129-Cellular Changes associated with Herpes
virus:virus:
10X picture of Herpes group infection.10X picture of Herpes group infection.
Transformed squamous cells withTransformed squamous cells with
cytomegaly, multi-nucleation, moldedcytomegaly, multi-nucleation, molded
nuclei with ground glass appearance.nuclei with ground glass appearance.
Intra nuclear inclusions and perinuclearIntra nuclear inclusions and perinuclear
clearing.clearing.
Inflammatory cells are present.Inflammatory cells are present.
130-Cellular Changes associated with Herpes130-Cellular Changes associated with Herpes
virus:virus:
40X picture of Herpes group infection.40X picture of Herpes group infection.
Transformed squamous cells withTransformed squamous cells with
cytomegaly, multi-nucleation, moldedcytomegaly, multi-nucleation, molded
nuclei with ground glass appearance.nuclei with ground glass appearance.
Intra nuclear inclusions and perinuclearIntra nuclear inclusions and perinuclear
clearing.clearing.
Inflammatory cells are present.Inflammatory cells are present.
LGM International,
13. BENIGN CELLULAR CHANGESBENIGN CELLULAR CHANGES
131-Cellular Changes associated with Herpes131-Cellular Changes associated with Herpes
virus:virus:
40X picture of Herpes group infection.40X picture of Herpes group infection.
Transformed squamous cells withTransformed squamous cells with
cytomegaly, multi-nucleationcytomegaly, multi-nucleation (1)(1),,
molded nuclei with ground glassmolded nuclei with ground glass
appearance.appearance.
Intra nuclear inclusions and perinuclearIntra nuclear inclusions and perinuclear
clearing.clearing.
Inflammatory cells are present.Inflammatory cells are present.
132-Actinomyces species:132-Actinomyces species:
40X picture of an infection of a IUD.40X picture of an infection of a IUD.
Delicate filamentous bacterium whichDelicate filamentous bacterium which
branch freely, resembling fungal hyphaebranch freely, resembling fungal hyphae
in loose aggregates.in loose aggregates.
Close observations show denseClose observations show dense
basophilic balls.basophilic balls.
Inflammatory cells are present.Inflammatory cells are present.
LGM International,
1
14. BENIGN CELLULAR CHANGESBENIGN CELLULAR CHANGES
133-Actinomycetes species.:133-Actinomycetes species.:
10X picture of an infection of a IUD.10X picture of an infection of a IUD.
Delicate filamentous bacterium whichDelicate filamentous bacterium which
branch freely, resembling fungal hyphaebranch freely, resembling fungal hyphae
in loose aggregates.in loose aggregates.
Close observations show denseClose observations show dense
basophilic balls.basophilic balls.
Inflammatory cells are present.Inflammatory cells are present.
134-Actinomycetes species:134-Actinomycetes species:
40X picture of an infection of a IUD.40X picture of an infection of a IUD.
Delicate filamentous bacterium whichDelicate filamentous bacterium which
branch freely, resembling fungalbranch freely, resembling fungal
hyphae in loose aggregates.hyphae in loose aggregates.
Close observations show denseClose observations show dense
basophilic balls.basophilic balls.
Inflammatory cells are present.Inflammatory cells are present.
LGM International,
15. BENIGN CELLULAR CHANGESBENIGN CELLULAR CHANGES
136A-Atrophic Cells.:136A-Atrophic Cells.:
40X magnification of a cervical sample in40X magnification of a cervical sample in
atrophy.atrophy.
Small, rounded, this basophilic parabasalSmall, rounded, this basophilic parabasal
squamous cells is typical of Atrophicsquamous cells is typical of Atrophic
cells. Atrophic cellcells. Atrophic cell (1)(1)
139-Changes due to Radiation:139-Changes due to Radiation:
40X magnification shows squamous cells40X magnification shows squamous cells
with fine vacuolization of the cytoplasm,with fine vacuolization of the cytoplasm,
cytomegaly, kariomegaly.cytomegaly, kariomegaly.
Nuclei are multinucleated with wrinklingNuclei are multinucleated with wrinkling
of the nuclear membrane.of the nuclear membrane.
Pleomorphic shapes and smudgedPleomorphic shapes and smudged
nuclear chromatin are observed alongnuclear chromatin are observed along
with Inflammatory cells and amorphouswith Inflammatory cells and amorphous
debris.debris.
LGM International,
1
16. BENIGN CELLULAR CHANGESBENIGN CELLULAR CHANGES
139A-Para-Keratosis:139A-Para-Keratosis:
40x magnification of mature squamous40x magnification of mature squamous
cells with hyper-keratinized cytoplasmcells with hyper-keratinized cytoplasm
and pyknotic nuclei.and pyknotic nuclei.
Consistent with uterine prolapse.Consistent with uterine prolapse.
139B-Candida albicans:139B-Candida albicans:
40x magnification of squamous cells and40x magnification of squamous cells and
multiple yeast forms with budding ormultiple yeast forms with budding or
delicate pseudo septate filamentsdelicate pseudo septate filaments
(pseudo-hyphae).(pseudo-hyphae).
Acute branching intra and extra cellular.Acute branching intra and extra cellular.
LGM International,
17. BENIGN CELLULAR CHANGESBENIGN CELLULAR CHANGES
139C-Candida albicans:139C-Candida albicans:
40x magnification of squamous cells and40x magnification of squamous cells and
multiple yeast forms with budding ormultiple yeast forms with budding or
delicate pseudo septate filamentsdelicate pseudo septate filaments
(pseudo-hyphae(pseudo-hyphae (1)(1)).).
Acute branching intra and extra cellular.Acute branching intra and extra cellular.
Few Inflammatory cells are noted.Few Inflammatory cells are noted.
140A-Benign Endometrial Cell:140A-Benign Endometrial Cell:
40x magnification of cell mass with40x magnification of cell mass with
double contouring, compact centraldouble contouring, compact central
stromal cells.stromal cells.
Peripheral glandular cells, surrounded byPeripheral glandular cells, surrounded by
Inflammatory cells.Inflammatory cells.
The glandular cells are round to oval,The glandular cells are round to oval,
scant cytoplasm and small nuclei.scant cytoplasm and small nuclei.
Nucleoli indistinct with coarsely granularNucleoli indistinct with coarsely granular
to compact chromatin.to compact chromatin. LGM International,
1
18. BENIGN CELLULAR CHANGESBENIGN CELLULAR CHANGES
126-Post coital smear:126-Post coital smear:
40x magnification picture of spermatozoa40x magnification picture of spermatozoa
(1)(1), histio-cytic cells and superficial, histio-cytic cells and superficial
cells.cells.
The spermatozoa has a long tail andThe spermatozoa has a long tail and
pointed pear shaped head.pointed pear shaped head.
The distal half of the head is pale and theThe distal half of the head is pale and the
portion adjacent to the tail is stainedportion adjacent to the tail is stained
dark.dark.
LGM International,
1
19. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
141-ASCUS:141-ASCUS:
40x magnification of atypical squamous40x magnification of atypical squamous
cells (of undetermined significance) withcells (of undetermined significance) with
enlargement of the nuclear size.enlargement of the nuclear size.
The increase in nuclear size is moreThe increase in nuclear size is more
marked than reactive changes and lessmarked than reactive changes and less
than LSIL.than LSIL.
Nucleus size are of intermediated andNucleus size are of intermediated and
parabasal cells or of mature metaplasticparabasal cells or of mature metaplastic
cellscells..
142-ASCUS:142-ASCUS:
40x magnification of atypical squamous40x magnification of atypical squamous
cells (of undetermined significance) withcells (of undetermined significance) with
enlargement of the nuclear size.enlargement of the nuclear size.
The increase in nuclear size is moreThe increase in nuclear size is more
marked than reactive changes and lessmarked than reactive changes and less
than LSIL.than LSIL.
Nucleus size are of intermediated andNucleus size are of intermediated and
parabasal cells or of mature metaplasticparabasal cells or of mature metaplastic
cells.cells.
LGM International,
20. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
144-ASCUS:144-ASCUS:
40x magnification atypical squamous40x magnification atypical squamous
cells (of undetermined significance) withcells (of undetermined significance) with
enlargement of the nuclear size.enlargement of the nuclear size.
The increase in nuclear size is moreThe increase in nuclear size is more
marked than reactive changes and lessmarked than reactive changes and less
than LSIL.than LSIL.
Nucleus size are of intermediated andNucleus size are of intermediated and
parabasal cells or of mature metaplasticparabasal cells or of mature metaplastic
cells.cells.
145-ASCUS:145-ASCUS:
40x magnification atypical squamous40x magnification atypical squamous
cells (of undetermined significance) withcells (of undetermined significance) with
enlargement of the nuclear size.enlargement of the nuclear size.
The increase in nuclear size is moreThe increase in nuclear size is more
marked than reactive changes and lessmarked than reactive changes and less
than LSIL.than LSIL.
Nucleus size are of intermediated andNucleus size are of intermediated and
parabasal cells or of mature metaplasticparabasal cells or of mature metaplastic
cells.cells.
LGM International,
21. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
146-ASCUS:146-ASCUS:
40x magnification atypical squamous40x magnification atypical squamous
cells (of undetermined significance)cells (of undetermined significance)
with enlargement of the nuclear size.with enlargement of the nuclear size.
The increase in nuclear size is moreThe increase in nuclear size is more
marked than reactive changes and lessmarked than reactive changes and less
than LSIL.than LSIL.
Nucleus size are of intermediated andNucleus size are of intermediated and
parabasal cells or of matureparabasal cells or of mature
metaplastic cells.metaplastic cells.
147-ASCUS:147-ASCUS:
40x magnification atypical squamous cells40x magnification atypical squamous cells
(of undetermined significance) with(of undetermined significance) with
enlargement of the nuclear size.enlargement of the nuclear size.
The increase in nuclear size is moreThe increase in nuclear size is more
marked than reactive changes and lessmarked than reactive changes and less
than LSIL.than LSIL.
Nucleus size are of intermediated andNucleus size are of intermediated and
parabasal cells or of mature metaplasticparabasal cells or of mature metaplastic
cells.cells.
LGM International,
22. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
149-ASCUS:149-ASCUS:
40x magnification of atypical squamous40x magnification of atypical squamous
cells (of undetermined significance) withcells (of undetermined significance) with
enlargement of the nuclear size.enlargement of the nuclear size.
The increase in nuclear size is moreThe increase in nuclear size is more
marked than reactive changes and lessmarked than reactive changes and less
than LSIL.than LSIL.
Nucleus size are of intermediated andNucleus size are of intermediated and
parabasal cells or of mature metaplasticparabasal cells or of mature metaplastic
cells.cells.
150-ASCUS:150-ASCUS:
40x magnification of atypical squamous40x magnification of atypical squamous
cells (of undetermined significance) withcells (of undetermined significance) with
enlargement of the nuclear size.enlargement of the nuclear size.
The increase in nuclear size is moreThe increase in nuclear size is more
marked than reactive changes and lessmarked than reactive changes and less
than LSIL.than LSIL.
Nucleus size are of intermediated andNucleus size are of intermediated and
parabasal cells or of mature metaplasticparabasal cells or of mature metaplastic
cells.cells.
LGM International,
23. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
153-ASCUS:153-ASCUS:
40x magnification atypical squamous40x magnification atypical squamous
cells (of undetermined significance) withcells (of undetermined significance) with
enlargement of the nuclear size.enlargement of the nuclear size.
The increase in nuclear size is moreThe increase in nuclear size is more
marked than reactive changes and lessmarked than reactive changes and less
than LSIL.than LSIL.
Nucleus size are of intermediated andNucleus size are of intermediated and
parabasal cells or of mature metaplasticparabasal cells or of mature metaplastic
cells.cells.
LGM International,
24. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
157-LSIL:157-LSIL:
40x magnification of low grade40x magnification of low grade
squamous intra-epithelial lesion/mildsquamous intra-epithelial lesion/mild
dysplasia/CIN I. Intermediatedysplasia/CIN I. Intermediate
squamous cells with enlargement ofsquamous cells with enlargement of
the nuclei.the nuclei.
Hyperchromasia, elevated N/C ratio (3Hyperchromasia, elevated N/C ratio (3
to 4 times the normal intermediateto 4 times the normal intermediate
cells) , no nucleoli. Coarsely granulatedcells) , no nucleoli. Coarsely granulated
chromatin.chromatin.
Cytoplasm is abundant, transparent.Cytoplasm is abundant, transparent.
158-LSIL:158-LSIL:
10x magnification of low grade10x magnification of low grade
squamous intra-epithelial lesion/mildsquamous intra-epithelial lesion/mild
dysplasia/CIN I. Intermediate squamousdysplasia/CIN I. Intermediate squamous
cells with enlargement of the nuclei.cells with enlargement of the nuclei.
Hyperchromasia, elevated N/C ratio (3 toHyperchromasia, elevated N/C ratio (3 to
4 times the normal intermediate cells) ,4 times the normal intermediate cells) ,
no nucleoli. Coarsely granulatedno nucleoli. Coarsely granulated
chromatin.chromatin.
Cytoplasm is abundant, transparent.Cytoplasm is abundant, transparent.
LGM International,
25. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
159-LSIL:159-LSIL:
40x magnification of low grade40x magnification of low grade
squamous intra-epithelial lesion/mildsquamous intra-epithelial lesion/mild
dysplasia/CIN I. Intermediatedysplasia/CIN I. Intermediate
squamous cells with enlargement ofsquamous cells with enlargement of
the nuclei.the nuclei.
Hyperchromasia, elevated N/C ratio (3Hyperchromasia, elevated N/C ratio (3
to 4 times the normal intermediateto 4 times the normal intermediate
cells) , no nucleoli. Coarselycells) , no nucleoli. Coarsely
granulated chromatin.granulated chromatin.
Cytoplasm is abundant, transparent.Cytoplasm is abundant, transparent.
160-LSIL:160-LSIL:
10x magnification of low grade10x magnification of low grade
squamous intra-epithelial lesion/mildsquamous intra-epithelial lesion/mild
dysplasia/CIN I. Intermediate squamousdysplasia/CIN I. Intermediate squamous
cells with enlargement of the nuclei.cells with enlargement of the nuclei.
Hyperchromasia, elevated N/C ratio (3 toHyperchromasia, elevated N/C ratio (3 to
4 times the normal intermediate cells) ,4 times the normal intermediate cells) ,
no nucleoli. Coarsely granulatedno nucleoli. Coarsely granulated
chromatin.chromatin.
Cytoplasm is abundant, transparent.Cytoplasm is abundant, transparent.
LGM International,
26. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
161-LSIL:161-LSIL:
40x magnification of low grade40x magnification of low grade
squamous intra-epithelial lesion/mildsquamous intra-epithelial lesion/mild
dysplasia/CIN I. Intermediatedysplasia/CIN I. Intermediate
squamous cells with enlargement ofsquamous cells with enlargement of
the nuclei.the nuclei.
Hyperchromasia, elevated N/C ratio (3Hyperchromasia, elevated N/C ratio (3
to 4 times the normal intermediateto 4 times the normal intermediate
cells) , no nucleoli. Coarsely granulatedcells) , no nucleoli. Coarsely granulated
chromatin.chromatin.
Cytoplasm is abundant, transparent.Cytoplasm is abundant, transparent.
162-LSIL:162-LSIL:
40x magnification of low grade40x magnification of low grade
squamous intra-epithelial lesion/mildsquamous intra-epithelial lesion/mild
dysplasia/CIN I. Intermediate squamousdysplasia/CIN I. Intermediate squamous
cells with enlargement of the nuclei.cells with enlargement of the nuclei.
Hyperchromasia, elevated N/C ratio (3 toHyperchromasia, elevated N/C ratio (3 to
4 times the normal intermediate cells) ,4 times the normal intermediate cells) ,
no nucleoli. Coarsely granulatedno nucleoli. Coarsely granulated
chromatin.chromatin.
Cytoplasm is abundant, transparent.Cytoplasm is abundant, transparent.
LGM International,
27. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
163-LSIL:163-LSIL:
40x magnification of low grade40x magnification of low grade
squamous intra-epithelial lesion/mildsquamous intra-epithelial lesion/mild
dysplasia/CIN I. Intermediate squamousdysplasia/CIN I. Intermediate squamous
cells with enlargement of the nuclei.cells with enlargement of the nuclei.
Hyperchromasia, elevated N/C ratio (3 toHyperchromasia, elevated N/C ratio (3 to
4 times the normal intermediate cells) ,4 times the normal intermediate cells) ,
no nucleoli. Coarsely granulatedno nucleoli. Coarsely granulated
chromatin.chromatin.
Cytoplasm is abundant, transparent.Cytoplasm is abundant, transparent.
LGM International,
28. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
166-HSIL:166-HSIL:
40x magnification of cells in loosely cohesive40x magnification of cells in loosely cohesive
groups, parabasal, metaplastic or basalgroups, parabasal, metaplastic or basal
type.type.
The cells are round, oval (size of parabasalThe cells are round, oval (size of parabasal
cells), the cell borders are rigid.cells), the cell borders are rigid.
The nuclei are enlarged and hyperchromatic,The nuclei are enlarged and hyperchromatic,
high N/C ratio.high N/C ratio.
The chromatin is fine to coarsely clumped.The chromatin is fine to coarsely clumped.
The nuclear membranes are irregular.The nuclear membranes are irregular.
Nucleoli are absent.Nucleoli are absent.
The cytoplasm is variable, scant toThe cytoplasm is variable, scant to
moderate.moderate.
167-HSIL :167-HSIL :
40x magnification of cells in loosely cohesive40x magnification of cells in loosely cohesive
groups, parabasal, metaplastic or basal type.groups, parabasal, metaplastic or basal type.
The cells are round, oval (size of parabasalThe cells are round, oval (size of parabasal
cells), the cell borders are rigid.cells), the cell borders are rigid.
The nuclei are enlarged and hyperchromatic,The nuclei are enlarged and hyperchromatic,
high N/C ratio.high N/C ratio.
The chromatin is fine to coarsely clumped.The chromatin is fine to coarsely clumped.
The nuclear membranes are irregular. NucleoliThe nuclear membranes are irregular. Nucleoli
are absent.are absent.
The cytoplasm is variable, scant to moderate.The cytoplasm is variable, scant to moderate.
LGM International,
29. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
169-HSIL:169-HSIL:
40x magnification cells in loosely cohesive40x magnification cells in loosely cohesive
groups, parabasal, metaplastic or basal type.groups, parabasal, metaplastic or basal type.
The cells are round, oval (size of parabasalThe cells are round, oval (size of parabasal
cells), the cell borders are rigid.cells), the cell borders are rigid.
The nuclei are enlarged and hyperchromatic,The nuclei are enlarged and hyperchromatic,
high N/C ratio.high N/C ratio.
The chromatin is fine to coarsely clumped.The chromatin is fine to coarsely clumped.
The nuclear membranes are irregular. NucleoliThe nuclear membranes are irregular. Nucleoli
are absent.are absent.
The cytoplasm is variable, scant to moderate.The cytoplasm is variable, scant to moderate.
171-HSIL:171-HSIL:
40x magnification of cells in loosely cohesive40x magnification of cells in loosely cohesive
groups, parabasal, metaplastic or basal type.groups, parabasal, metaplastic or basal type.
The cells are round, oval (size of parabasalThe cells are round, oval (size of parabasal
cells), the cell borders are rigid.cells), the cell borders are rigid.
The nuclei are enlarged and hyperchromatic,The nuclei are enlarged and hyperchromatic,
high N/C ratio.high N/C ratio.
The chromatin is fine to coarsely clumped.The chromatin is fine to coarsely clumped.
The nuclear membranes are irregular. NucleoliThe nuclear membranes are irregular. Nucleoli
are absent.are absent.
The cytoplasm is variable, scant to moderate.The cytoplasm is variable, scant to moderate.
LGM International,
30. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
173-HSIL :173-HSIL :
40x magnification of cells in loosely cohesive40x magnification of cells in loosely cohesive
groups, parabasal, metaplastic or basal type.groups, parabasal, metaplastic or basal type.
The cells are round, oval (size of parabasalThe cells are round, oval (size of parabasal
cells), the cell borders are rigid.cells), the cell borders are rigid.
The nuclei are enlarged and hyperchromatic,The nuclei are enlarged and hyperchromatic,
high N/C ratio.high N/C ratio.
The chromatin is fine to coarsely clumped.The chromatin is fine to coarsely clumped.
The nuclear membranes are irregular. NucleoliThe nuclear membranes are irregular. Nucleoli
are absent.are absent.
The cytoplasm is variable, scant to moderate.The cytoplasm is variable, scant to moderate.
176-HSIL:176-HSIL:
40x magnification of cells in loosely cohesive40x magnification of cells in loosely cohesive
groups, parabasal, metaplastic or basal type.groups, parabasal, metaplastic or basal type.
The cells are round, oval (size of parabasalThe cells are round, oval (size of parabasal
cells), the cell borders are rigid.cells), the cell borders are rigid.
The nuclei are enlarged and hyperchromatic,The nuclei are enlarged and hyperchromatic,
high N/C ratio.high N/C ratio.
The chromatin is fine to coarsely clumped.The chromatin is fine to coarsely clumped.
The nuclear membranes are irregular. NucleoliThe nuclear membranes are irregular. Nucleoli
are absent.are absent.
The cytoplasm is variable, scant to moderate.The cytoplasm is variable, scant to moderate.
LGM International,
31. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
180-HSIL:180-HSIL:
40x magnification of cells in loosely cohesive40x magnification of cells in loosely cohesive
groups, parabasal, metaplastic or basal type.groups, parabasal, metaplastic or basal type.
The cells are round, oval (size of parabasalThe cells are round, oval (size of parabasal
cells), the cell borders are rigid.cells), the cell borders are rigid.
The nuclei are enlarged and hyperchromatic,The nuclei are enlarged and hyperchromatic,
high N/C ratio.high N/C ratio.
The chromatin is fine to coarsely clumped.The chromatin is fine to coarsely clumped.
The nuclear membranes are irregular.The nuclear membranes are irregular.
Nucleoli are absent.Nucleoli are absent.
The cytoplasm is variable, scant to moderate.The cytoplasm is variable, scant to moderate.
181-HSIL:181-HSIL:
40x magnification cells in loosely cohesive40x magnification cells in loosely cohesive
groups, parabasal, metaplastic or basal type.groups, parabasal, metaplastic or basal type.
The cells are round, oval (size of parabasalThe cells are round, oval (size of parabasal
cells), the cell borders are rigid.cells), the cell borders are rigid.
The nuclei are enlarged and hyperchromatic,The nuclei are enlarged and hyperchromatic,
high N/C ratio.high N/C ratio.
The chromatin is fine to coarsely clumped.The chromatin is fine to coarsely clumped.
The nuclear membranes are irregular. NucleoliThe nuclear membranes are irregular. Nucleoli
are absent.are absent.
The cytoplasm is variable, scant to moderate.The cytoplasm is variable, scant to moderate.
LGM International,
32. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
182-HSIL:182-HSIL:
40x magnification of cells in loosely cohesive40x magnification of cells in loosely cohesive
groups, parabasal, metaplastic or basal type.groups, parabasal, metaplastic or basal type.
The cells are round, oval (size of parabasalThe cells are round, oval (size of parabasal
cells), the cell borders are rigid.cells), the cell borders are rigid.
The nuclei are enlarged and hyperchromatic,The nuclei are enlarged and hyperchromatic,
high N/C ratio.high N/C ratio.
The chromatin is fine to coarsely clumped.The chromatin is fine to coarsely clumped.
The nuclear membranes are irregular. NucleoliThe nuclear membranes are irregular. Nucleoli
are absent.are absent.
The cytoplasm is variable, scant to moderate.The cytoplasm is variable, scant to moderate.
186-HSIL:186-HSIL:
40x magnification cells in loosely cohesive40x magnification cells in loosely cohesive
groups, parabasal, metaplastic or basal type.groups, parabasal, metaplastic or basal type.
The cells are round, oval (size of parabasalThe cells are round, oval (size of parabasal
cells), the cell borders are rigid.cells), the cell borders are rigid.
The nuclei are enlarged and hyperchromatic,The nuclei are enlarged and hyperchromatic,
high N/C ratio.high N/C ratio.
The chromatin is fine to coarsely clumped.The chromatin is fine to coarsely clumped.
The nuclear membranes are irregular. NucleoliThe nuclear membranes are irregular. Nucleoli
are absent.are absent.
The cytoplasm is variable, scant to moderate.The cytoplasm is variable, scant to moderate.
LGM International,
33. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
188-HSIL:188-HSIL:
40x magnification cells in loosely cohesive40x magnification cells in loosely cohesive
groups, parabasal, metaplastic or basal type.groups, parabasal, metaplastic or basal type.
The cells are round, oval (size of parabasalThe cells are round, oval (size of parabasal
cells), the cell borders are rigid.cells), the cell borders are rigid.
The nuclei are enlarged and hyperchromatic,The nuclei are enlarged and hyperchromatic,
high N/C ratio.high N/C ratio.
The chromatin is fine to coarsely clumped.The chromatin is fine to coarsely clumped.
The nuclear membranes are irregular.The nuclear membranes are irregular.
Nucleoli are absent.Nucleoli are absent.
The cytoplasm is variable, scant to moderate.The cytoplasm is variable, scant to moderate.
189-HSIL:189-HSIL:
40x magnification of cells in loosely cohesive40x magnification of cells in loosely cohesive
groups, parabasal, metaplastic or basal type.groups, parabasal, metaplastic or basal type.
The cells are round, oval (size of parabasalThe cells are round, oval (size of parabasal
cells), the cell borders are rigid.cells), the cell borders are rigid.
The nuclei are enlarged and hyperchromatic,The nuclei are enlarged and hyperchromatic,
high N/C ratio.high N/C ratio.
The chromatin is fine to coarsely clumped.The chromatin is fine to coarsely clumped.
The nuclear membranes are irregular. NucleoliThe nuclear membranes are irregular. Nucleoli
are absent.are absent.
The cytoplasm is variable, scant to moderate.The cytoplasm is variable, scant to moderate.
LGM International,
34. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
193-Squamous Cell Carcinoma poorly193-Squamous Cell Carcinoma poorly
differentiated:differentiated:
40x magnification of malignant cells in loosely40x magnification of malignant cells in loosely
cohesive groups, mostly round, polygonal,cohesive groups, mostly round, polygonal,
size variable.size variable.
The borders are not well defined.The borders are not well defined.
The nuclei are large, hyper-chromatic.The nuclei are large, hyper-chromatic.
The nuclear membranes are smooth toThe nuclear membranes are smooth to
irregular, the chromatin is coarsely granularirregular, the chromatin is coarsely granular
having irregular chromocenters.having irregular chromocenters.
Micronucleoli are seen.Micronucleoli are seen.
The cytoplasm is scant. No Keratinization isThe cytoplasm is scant. No Keratinization is
present.present.
The N/C ratio is high.The N/C ratio is high.
195-Squamous Cell Carcinoma:195-Squamous Cell Carcinoma:
40x magnification of cells isolated, in loosely40x magnification of cells isolated, in loosely
cohesive groups and syncytial arrangement.cohesive groups and syncytial arrangement.
Marked pleomorphism with variation in size andMarked pleomorphism with variation in size and
shape (small, round, oval, oblong to elongatedshape (small, round, oval, oblong to elongated
(spindle), caudate and tadpole.(spindle), caudate and tadpole.
Cells wrapping around adjacent cells are present.Cells wrapping around adjacent cells are present.
The nuclear are enlarged and hyperchromatic.The nuclear are enlarged and hyperchromatic.
The nuclear membranes are smooth to irregular. TheThe nuclear membranes are smooth to irregular. The
chromatin is dark stained. Micronucleoili are present.chromatin is dark stained. Micronucleoili are present.
The cell borders are rigid and not well defined. TheThe cell borders are rigid and not well defined. The
cytoplasm is variable (scant to abundant).cytoplasm is variable (scant to abundant).
LGM International,
35. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
196-Squamous Cell Carcinoma:196-Squamous Cell Carcinoma:
40x magnification of cells isolated, in loosely40x magnification of cells isolated, in loosely
cohesive groups and syncytial arrangement.cohesive groups and syncytial arrangement.
Marked pleomorphism with variation in size andMarked pleomorphism with variation in size and
shape (small, round, oval, oblong to elongatedshape (small, round, oval, oblong to elongated
(spindle), caudate and tadpole(spindle), caudate and tadpole (1)(1)..
Cells wrapping around adjacent cells areCells wrapping around adjacent cells are
present.present.
The nuclear are enlarged and hyperchromatic.The nuclear are enlarged and hyperchromatic.
The nuclear membranes are smooth toThe nuclear membranes are smooth to
irregular. The chromatin is dark stained.irregular. The chromatin is dark stained.
Micronucleoili are present.Micronucleoili are present.
The cell borders are rigid and not well defined.The cell borders are rigid and not well defined.
The cytoplasm is variable (scant to abundant)The cytoplasm is variable (scant to abundant)
198-Squamous Cell Carcinoma:198-Squamous Cell Carcinoma:
40x magnification of cells isolated, in loosely40x magnification of cells isolated, in loosely
cohesive groups and syncytial arrangement.cohesive groups and syncytial arrangement.
Marked pleomorphism with variation in size andMarked pleomorphism with variation in size and
shape (small, round, oval, oblong to elongatedshape (small, round, oval, oblong to elongated
(spindle)(spindle) (1),(1), caudate and tadpole.caudate and tadpole.
Keratinized squamous pearls and cells wrappingKeratinized squamous pearls and cells wrapping
around adjacent cells are present.around adjacent cells are present.
The nuclear are enlarged and hyperchromatic.The nuclear are enlarged and hyperchromatic.
The nuclear membranes are smooth to irregular. TheThe nuclear membranes are smooth to irregular. The
chromatin is dark stained. Micronucleoili are present.chromatin is dark stained. Micronucleoili are present.
The cell borders are rigid and not well defined. TheThe cell borders are rigid and not well defined. The
cytoplasm is variable (scant to abundant)cytoplasm is variable (scant to abundant)
LGM International,
1
1
36. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
200-Squamous Cell Carcinoma:200-Squamous Cell Carcinoma:
40x magnification of cells isolated, in loosely40x magnification of cells isolated, in loosely
cohesive groups and syncytial arrangement.cohesive groups and syncytial arrangement.
Marked pleomorphism with variation in size andMarked pleomorphism with variation in size and
shape (small, round, oval, oblong to elongatedshape (small, round, oval, oblong to elongated
(spindle), caudate and tadpole.(spindle), caudate and tadpole.
Keratinized squamous pearls and cells wrappingKeratinized squamous pearls and cells wrapping
around adjacent cells are present.around adjacent cells are present.
The nuclear are enlarged and hyperchromatic.The nuclear are enlarged and hyperchromatic.
The nuclear membranes are smooth toThe nuclear membranes are smooth to
irregular. The chromatin is dark stained.irregular. The chromatin is dark stained.
Micronucleoili are present.Micronucleoili are present.
The cell borders are rigid and not well defined.The cell borders are rigid and not well defined.
The cytoplasm is variable (scant to abundant)The cytoplasm is variable (scant to abundant)
201-Squamous Cell Carcinoma:201-Squamous Cell Carcinoma:
40x magnification cells isolated, in loosely cohesive40x magnification cells isolated, in loosely cohesive
groups and syncytial arrangement.groups and syncytial arrangement.
Marked pleomorphism with variation in size andMarked pleomorphism with variation in size and
shape (small, round, oval, oblong to elongatedshape (small, round, oval, oblong to elongated
(spindle), caudate and tadpole.(spindle), caudate and tadpole.
Cells wrapping around adjacent cells are present.Cells wrapping around adjacent cells are present.
The nuclear are enlarged and hyperchromatic.The nuclear are enlarged and hyperchromatic.
The nuclear membranes are smooth to irregular.The nuclear membranes are smooth to irregular.
The chromatin is dark stained. Micronucleoili areThe chromatin is dark stained. Micronucleoili are
present.present.
The cell borders are rigid and not well defined. TheThe cell borders are rigid and not well defined. The
cytoplasm is variable (scant to abundant)cytoplasm is variable (scant to abundant)
LGM International,
37. SQUAMOUS CELL ABNORMALITIESSQUAMOUS CELL ABNORMALITIES
202-Squamous Cell Carcinoma:202-Squamous Cell Carcinoma:
40x magnification cells isolated, in loosely40x magnification cells isolated, in loosely
cohesive groups and syncytial arrangement.cohesive groups and syncytial arrangement.
Marked pleomorphism with variation in size andMarked pleomorphism with variation in size and
shape (small, round, oval, oblong to elongatedshape (small, round, oval, oblong to elongated
(spindle), caudate and tadpole.(spindle), caudate and tadpole.
Cells wrapping around adjacent cells areCells wrapping around adjacent cells are
present.present.
The nuclear are enlarged and hyperchromatic.The nuclear are enlarged and hyperchromatic.
The nuclear membranes are smooth toThe nuclear membranes are smooth to
irregular. The chromatin is dark stained.irregular. The chromatin is dark stained.
Micronucleoili are present.Micronucleoili are present.
The cell borders are rigid and not well defined.The cell borders are rigid and not well defined.
The cytoplasm is variable (scant to abundant)The cytoplasm is variable (scant to abundant)
LGM International,
38. GLANDULAR ABNORMALITIESGLANDULAR ABNORMALITIES
204-Endocervical adenocarcinoma (morules):204-Endocervical adenocarcinoma (morules):
40x magnification of cells in tissue fragments,40x magnification of cells in tissue fragments,
loosely cohesive groups and syncythialloosely cohesive groups and syncythial
arrangement with acinar pattern, branching,arrangement with acinar pattern, branching,
glandular like (nuclei around the opening withglandular like (nuclei around the opening with
vertical polarity, nuclei at the peripheryvertical polarity, nuclei at the periphery
stringing out with a feathering pattern andstringing out with a feathering pattern and
palisading of nucleipalisading of nuclei
The nuclei are round, oval, central andThe nuclei are round, oval, central and
paracentric, variable size, hyperchromatic, theparacentric, variable size, hyperchromatic, the
chromatin is coarsely granular.chromatin is coarsely granular.
Loss of nuclear polarity.Loss of nuclear polarity.
Micronucleoli are present.Micronucleoli are present.
The cytoplasm is variable and micro-The cytoplasm is variable and micro-
vacuolization is seen.vacuolization is seen.
205-Endocervical adenocarcinoma:205-Endocervical adenocarcinoma:
40x magnification of cells in tissue fragments,40x magnification of cells in tissue fragments,
loosely cohesive groups and syncythialloosely cohesive groups and syncythial
arrangement with acinar pattern, branching,arrangement with acinar pattern, branching,
glandular like (nuclei around the opening withglandular like (nuclei around the opening with
vertical polarity, nuclei at the peripheryvertical polarity, nuclei at the periphery
stringing out with a feathering pattern andstringing out with a feathering pattern and
palisading of nucleipalisading of nuclei
The nuclei are round, oval, central andThe nuclei are round, oval, central and
paracentric, variable size, hyperchromatic, theparacentric, variable size, hyperchromatic, the
chromatin is coarsely granular.chromatin is coarsely granular.
Loss of nuclear polarity.Loss of nuclear polarity.
Micronucleoli are present.Micronucleoli are present.
The cytoplasm is variable and micro-The cytoplasm is variable and micro-
vacuolization is seen.vacuolization is seen. LGM International,
39. GLANDULAR ABNORMALITIESGLANDULAR ABNORMALITIES
206-Endocervical adenocarcinoma (Morules):206-Endocervical adenocarcinoma (Morules):
40x magnification of cells in tissue fragments,40x magnification of cells in tissue fragments,
loosely cohesive groups and syncythialloosely cohesive groups and syncythial
arrangement with acinar pattern, branching,arrangement with acinar pattern, branching,
glandular like (nuclei around the opening withglandular like (nuclei around the opening with
vertical polarity, nuclei at the peripheryvertical polarity, nuclei at the periphery
stringing out with a feathering pattern andstringing out with a feathering pattern and
palisading of nucleipalisading of nuclei
The nuclei are round, oval, central andThe nuclei are round, oval, central and
paracentric, variable size, hyperchromatic, theparacentric, variable size, hyperchromatic, the
chromatin is coarsely granular.chromatin is coarsely granular.
Loss of nuclear polarity.Loss of nuclear polarity.
Micronucleoli are present.Micronucleoli are present.
The cytoplasm is variable and micro-The cytoplasm is variable and micro-
vacuolization is seen.vacuolization is seen.
LGM International,
209-Endocervical adenocarcinoma (Syncytial):209-Endocervical adenocarcinoma (Syncytial):
40x magnification of cells in tissue fragments, loosely40x magnification of cells in tissue fragments, loosely
cohesive groups and syncythial arrangement withcohesive groups and syncythial arrangement with
acinar pattern, branching, glandular like (nucleiacinar pattern, branching, glandular like (nuclei
around the opening with vertical polarity, nuclei at thearound the opening with vertical polarity, nuclei at the
periphery stringing out with a feathering pattern andperiphery stringing out with a feathering pattern and
palisading of nucleipalisading of nuclei
The nuclei are round, oval, central and paracentric,The nuclei are round, oval, central and paracentric,
variable size, hyperchromatic, the chromatin isvariable size, hyperchromatic, the chromatin is
coarsely granular.coarsely granular.
Loss of nuclear polarity.Loss of nuclear polarity.
Micronucleoli are present.Micronucleoli are present.
The cytoplasm is variable and micro-vacuolization isThe cytoplasm is variable and micro-vacuolization is
seen.seen.
40. GLANDULAR ABNORMALITIESGLANDULAR ABNORMALITIES
208-Endocervical adenocarcinoma (Morules):208-Endocervical adenocarcinoma (Morules):
40x magnification of cells in tissue40x magnification of cells in tissue
fragments, loosely cohesive groups andfragments, loosely cohesive groups and
syncythial arrangement with acinar pattern,syncythial arrangement with acinar pattern,
branching, glandular like (nuclei around thebranching, glandular like (nuclei around the
opening with vertical polarity, nuclei at theopening with vertical polarity, nuclei at the
periphery stringing out with a featheringperiphery stringing out with a feathering
pattern and palisading of nucleipattern and palisading of nuclei
The nuclei are round, oval, central andThe nuclei are round, oval, central and
paracentric, variable size, hyperchromatic,paracentric, variable size, hyperchromatic,
the chromatin is coarsely granular.the chromatin is coarsely granular.
Loss of nuclear polarity.Loss of nuclear polarity.
Micronucleoli are present.Micronucleoli are present.
The cytoplasm is variable and micro-The cytoplasm is variable and micro-
vacuolization is seen.vacuolization is seen.
209-Endocervical adenocarcinoma (Syncytial):209-Endocervical adenocarcinoma (Syncytial):
40x magnification of cells in tissue fragments,40x magnification of cells in tissue fragments,
loosely cohesive groups and syncythial arrangementloosely cohesive groups and syncythial arrangement
with acinar pattern, branching, glandular like (nucleiwith acinar pattern, branching, glandular like (nuclei
around the opening with vertical polarity, nuclei ataround the opening with vertical polarity, nuclei at
the periphery stringing out with a feathering patternthe periphery stringing out with a feathering pattern
and palisading of nucleiand palisading of nuclei
The nuclei are round, oval, central and paracentric,The nuclei are round, oval, central and paracentric,
variable size, hyperchromatic, the chromatin isvariable size, hyperchromatic, the chromatin is
coarsely granular.coarsely granular.
Loss of nuclear polarity.Loss of nuclear polarity.
Micronucleoli are present.Micronucleoli are present.
The cytoplasm is variable and micro-vacuolization isThe cytoplasm is variable and micro-vacuolization is
seen.seen.
LGM International,
41. GLANDULAR ABNORMALITIESGLANDULAR ABNORMALITIES
210-Endocervical adenocarcinoma (Glandular210-Endocervical adenocarcinoma (Glandular
like):like):
40x magnification of cells in tissue40x magnification of cells in tissue
fragments, loosely cohesive groups andfragments, loosely cohesive groups and
syncythial arrangement with acinarsyncythial arrangement with acinar
pattern, branching, glandular like (nucleipattern, branching, glandular like (nuclei
around the opening with vertical polarity,around the opening with vertical polarity,
nuclei at the periphery stringing out with anuclei at the periphery stringing out with a
feathering pattern and palisading of nucleifeathering pattern and palisading of nuclei
The nuclei are round, oval, central andThe nuclei are round, oval, central and
paracentric, variable size, hyperchromatic,paracentric, variable size, hyperchromatic,
the chromatin is coarsely granular.the chromatin is coarsely granular.
Loss of nuclear polarity.Loss of nuclear polarity.
Micronucleoli are present.Micronucleoli are present.
The cytoplasm is variable and micro-The cytoplasm is variable and micro-
vacuolization is seen.vacuolization is seen.
211-Endocervical adenocarcinoma (Loosely cohesive211-Endocervical adenocarcinoma (Loosely cohesive
groups):groups):
40x magnification of cells in tissue fragments, loosely40x magnification of cells in tissue fragments, loosely
cohesive groups and syncythial arrangement withcohesive groups and syncythial arrangement with
acinar pattern, branching, glandular like (nucleiacinar pattern, branching, glandular like (nuclei
around the opening with vertical polarity, nuclei ataround the opening with vertical polarity, nuclei at
the periphery stringing out with a feathering patternthe periphery stringing out with a feathering pattern
and palisading of nucleiand palisading of nuclei
The nuclei are round, oval, central and paracentric,The nuclei are round, oval, central and paracentric,
variable size, hyperchromatic, the chromatin isvariable size, hyperchromatic, the chromatin is
coarsely granular.coarsely granular.
Loss of nuclear polarity.Loss of nuclear polarity.
Micronucleoli are present.Micronucleoli are present.
The cytoplasm is variable and micro-vacuolization isThe cytoplasm is variable and micro-vacuolization is
seen.seen. LGM International,
42. GLANDULAR ABNORMALITIESGLANDULAR ABNORMALITIES
212-Endocervical adenocarcinoma (loosely212-Endocervical adenocarcinoma (loosely
cohesive groups):cohesive groups):
40x magnification of cells in tissue40x magnification of cells in tissue
fragments, loosely cohesive groups andfragments, loosely cohesive groups and
syncythial arrangement with acinar pattern,syncythial arrangement with acinar pattern,
branching, glandular like (nuclei around thebranching, glandular like (nuclei around the
opening with vertical polarity, nuclei at theopening with vertical polarity, nuclei at the
periphery stringing out with a featheringperiphery stringing out with a feathering
pattern and palisading of nucleipattern and palisading of nuclei
The nuclei are round, oval, central andThe nuclei are round, oval, central and
paracentric, variable size, hyperchromatic,paracentric, variable size, hyperchromatic,
the chromatin is coarsely granular.the chromatin is coarsely granular.
Loss of nuclear polarity.Loss of nuclear polarity.
Micronucleoli are present.Micronucleoli are present.
The cytoplasm is variable and micro-The cytoplasm is variable and micro-
vacuolization is seen.vacuolization is seen.
213-Endocervical adenocarcinoma (loosely cohesive213-Endocervical adenocarcinoma (loosely cohesive
groups):groups):
40x magnification of cells in tissue fragments, loosely40x magnification of cells in tissue fragments, loosely
cohesive groups and syncythial arrangement withcohesive groups and syncythial arrangement with
acinar pattern, branching, glandular like (nucleiacinar pattern, branching, glandular like (nuclei
around the opening with vertical polarity, nuclei at thearound the opening with vertical polarity, nuclei at the
periphery stringing out with a feathering pattern andperiphery stringing out with a feathering pattern and
palisading of nucleipalisading of nuclei
The nuclei are round, oval, central and paracentric,The nuclei are round, oval, central and paracentric,
variable size, hyperchromatic, the chromatin isvariable size, hyperchromatic, the chromatin is
coarsely granular.coarsely granular.
Loss of nuclear polarity.Loss of nuclear polarity.
Micronucleoli are present.Micronucleoli are present.
The cytoplasm is variable and micro-vacuolization isThe cytoplasm is variable and micro-vacuolization is
seen.seen. LGM International,
43. GLANDULAR ABNORMALITIESGLANDULAR ABNORMALITIES
214-Endocervical adenocarcinoma (palisading):214-Endocervical adenocarcinoma (palisading):
40x magnification of cells in tissue fragments,40x magnification of cells in tissue fragments,
loosely cohesive groups and syncythialloosely cohesive groups and syncythial
arrangement with acinar pattern, branching,arrangement with acinar pattern, branching,
glandular like (nuclei around the opening withglandular like (nuclei around the opening with
vertical polarity, nuclei at the peripheryvertical polarity, nuclei at the periphery
stringing out with a feathering pattern andstringing out with a feathering pattern and
palisading of nucleipalisading of nuclei
The nuclei are round, oval, central andThe nuclei are round, oval, central and
paracentric, variable size, hyperchromatic, theparacentric, variable size, hyperchromatic, the
chromatin is coarsely granular.chromatin is coarsely granular.
Loss of nuclear polarity.Loss of nuclear polarity.
Micronucleoli are present.Micronucleoli are present.
The cytoplasm is variable and micro-The cytoplasm is variable and micro-
vacuolization is seen.vacuolization is seen.
LGM International,
44. NON GYN CYTOLOGYNON GYN CYTOLOGY
215-Thyroid: (40x magnification)215-Thyroid: (40x magnification)
Isolated follicular cells.Isolated follicular cells.
216-Thyroid: (40x magnification)216-Thyroid: (40x magnification)
Atypical cells grouped with irregular contours.Atypical cells grouped with irregular contours.
Nuclear inclusions and groovesNuclear inclusions and grooves
Finely granular chromatin and squamoidFinely granular chromatin and squamoid
cytoplasm.cytoplasm.
Nuclear enlargement, hyperchromaticNuclear enlargement, hyperchromatic
(suggestive of papillary carcinoma)(suggestive of papillary carcinoma)
LGM International,
45. NON GYN CYTOLOGYNON GYN CYTOLOGY
219-Urine smear: (40x magnification)219-Urine smear: (40x magnification)
Consistent with bladder urotelliumConsistent with bladder urotellium
Transitional epithelial cells in cohesive group.Transitional epithelial cells in cohesive group.
The cells are large and elliptical with abundantThe cells are large and elliptical with abundant
eosinophilic cytoplasmeosinophilic cytoplasm
Well defined borders.Well defined borders.
221-Thyroid: (40x magnification)221-Thyroid: (40x magnification)
Atypical cells grouped with irregular contours.Atypical cells grouped with irregular contours.
Nuclear inclusions and groovesNuclear inclusions and grooves
Finely granular chromatin and squamoidFinely granular chromatin and squamoid
cytoplasm.cytoplasm.
Nuclear enlargement, hyperchromaticNuclear enlargement, hyperchromatic
(suggestive of papillary carcinoma)(suggestive of papillary carcinoma)
LGM International,
46. NON GYN CYTOLOGYNON GYN CYTOLOGY
223-Pleural Fluids: (40x magnification)223-Pleural Fluids: (40x magnification)
Many small round cells with hyperchromatic nucleiMany small round cells with hyperchromatic nuclei
and very scant cytoplasm (consistent withand very scant cytoplasm (consistent with
lymphocytes.)lymphocytes.)
LGM International,
