1. Thermostable DNA polymerase
from thermophiles found in local
hot springs: Expression and
characterization
By Mohd Khairul Fadzly Haron
Supervised by: Prof. Dr. Raja Noor Zaliha Raja Abd. Rahman

2.  Selected 20 colonies
 Dilution streaking to get single colony
 Grow in nutrient broths 60°C
 Grow in nutrient broths 80°C (2 days)
 Spread on plates (nutrient agar)
 Dilution streaking
 Gram staining

4. Gram stain

5.  Sense primer
5’-GAT-CCG-AAC-CTG-CAA-AAC-ATT-CC-
3’
 Antisense primer
5’-CAG-GTG-CAT-GAC-GAG-CTG-ATT-3’
(Akhmaloka et al.,2008)