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Manual polybrene
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Manual Polybrene Method
張志昇
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Manual polybrene 注意事項
• 使用顯微鏡觀察 • 1mL LIM會比0.6mL LIM 較敏感些。 • 使用LIM先洗滌cell會比較敏感些。 • 使用0.05% Polybrene判讀時間勿超過1min • 洗腎病人使用多陽離子heparin, 或使用某些高 劑量帶陽離子藥物,不適合使用mp法, 若要 做抗體篩檢, 只能使用傳統AHG • multiple myloma的病人,LIM要加量。 • 對cold antibody 較敏感,也可使用MPA方法 區分或MPCAT。
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