3. Influenza H7N9 Reassortant
2011 2012 2013
H9N2 chicken
A/chicken/Zhejiang/611/2011
H7N9 wild bird
H7N3 duck
H9N2 chicken
A/chicken/Shanghai/C1/2012
H7N9 human
A/Shanghai/1/2013
H7N9 human
A/Shanghai/2/2013
A/Anhui/2/2013
A/duck/Zhejiang/12/2011
NA
HA
NP
内部遺伝子6本
Clyde Dapat, Division of International Health, Niigata University
4. HA遺伝子開裂部位
＝低病原性
リシン、アルギニンの連続配列の欠如
proteolytic cleavage motif
Absence of polybasic sequences (K and R) in the recent H7N9 indicates low
pathogenicity.
Clyde Dapat, Division of International Health, Niigata University
Data source : GISAID http://platform.gisaid.org/epi3/frontend#5f426b
5. HA mutations in Ferret Transmission Experiments
160位アラニン、226位ロイシン
H3 a.a. position (H5 no.)
110
(103)
158
160
(156)
224
226
(222)
228
(224)
318 PB2:627
A/H5N1 wild-type H N T N Q G T E
A/H5N1 mutant Y D A K L S I K
A/Shanghai/1/2013 (H7N9) Q N A N Q G T K
A/Shanghai/2/2013 (H7N9) Q N A N L G T K
A/Anhui/1/2013 (H7N9) Q N A N L G T K
A/Hangzhou/1/2013 (H7N9) Q N A N I* G T --
A/Chicken/Shanghai/S1053/2013 (H7N9) Q N A N L G T E
A/Environment/Shanghai/S1088/2013 (H7N9) Q N A N L G T E
A/Pigeon/Shanghai/S1069/2013 (H7N9) Q N A N L G T E
A/Netherlands/127/2003 (H7N7) Q N A N Q G T E
Fouchier et al. Science (2012) 336:1534 [HA:H103Y, T156A, Q222L, G224S and PB2:E627K]
Kawaoka et al. Nature (2012) 486:420 [N158D, N224K, Q226L, T318I]
*226I in human H3N2
Clyde Dapat, Division of International Health, Niigata University
Data source : GISAID http://platform.gisaid.org/epi3/frontend#5f426b
6. HA mutations mapping
T160A
Q226L I
H110Y
N158D
N224K
G228S
T318I
Present in H7N9
Clyde Dapat, Division of International Health, Niigata University
