1. +
Group 10: Angelli Vivas
DNA Isolation
Kim Valenzuela Kana Watabe
Janina Villaseran
2. +
Principles
DNA isolation is a process used to extract
DNA from various sources.
Ethanol is used to precipitate out the DNA and
then collected through DNA spooling.
3. + Procedures
Dissolve EDTA and
NaCl in 900 ml
Distilled H2O. Spool the DNA
from the bottom of
the test tube using
a stirring rod.
Add 100 ml Axion
liquid detergent.
Add 10 ml of 95%
chilled ethanol.
Blend onion
chunks with 100
ml of mixture.
Collect 5 ml of
filtered fluid and
place in test tube.
Heat for 30 mins
at 50 C.
Cool in an ice bath
for 10 mins and
filter.
4. + After completing the
process of DNA
isolation, a white
substance should
appear at the bottom
of the test tube.
Results
5. + The white stringy
substance extracted
after DNA spooling is
the DNA from the
onion.
Interpretations
