This document describes research into synthesizing a novel phospholipid layer/mesoporous carbon nanoparticles (MCNs) structure for efficient drug loading and delivery. MCNs were synthesized and coated with a phospholipid layer to control drug loading and release. Doxorubicin was loaded as a model drug and showed higher loading without the phospholipid coating, demonstrating the coating's role in controlling drug behavior. The MCN/phospholipid core/shell structure has potential as a next-generation drug nanocarrier for bio-medical applications.

1. Yuan-Yun Lin , Ling Chao*, Kevin C.-W. Wu*
Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan
Discussions
Objective
Acknowledgements
Characterization
SEM of MCNs
E-mail ：lingchao@ntu.edu.tw and kevinwu@ntu.edu.tw
Synthesis of A Novel Phospholipid Layer/Mesoporous Carbon Nanoparticles (MCNs)
Core/Shell Structure as An Anticancer Drug Nanovehicle
for Efficient Drug Loading
Synthesis of a Novel Phospholipid Layer/MCNs
Experimental
Doxorubicin
Hydrophobic Drug Loading
Lipid coating
MCNs
Vial DOPC@CCl4
Remove organic solvent
under N2
Mix well
Add solution
Drug Loading Testing
centrifugation
Optical MicroscopeFluorescence Photometer
Analysis
TEM of MCNs
50 nm
BET of MCNs
Relative Pressure (p/p0)
VolumeAbsorbed(cm3g-1)
Pore Diameter (nm)VP
0
50
100
150
200
250
300
350
0 0.2 0.4 0.6 0.8 1 1.2
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
0.5
0 50 100 150 200
Drug Loading
0
20
40
60
80
100
Loading(%)
Results
Because of their high surface area and the hydrophobicity,
mesoporous carbon nanoparticles (MCN) were successfully
synthesized and utilized as a potential drug nanocarrier. In
addition, a phospholipid layer was applied as a gate-keeper to
control the load/release behavior of drugs. The MCN/
phospholipid core/shell structure here provides a new
alternative for next-generation bio-medical applications.
The research was supported by the National Taiwan University.
Synthesis of a Novel Phospholipid Layer/Mesoporous Carbon Nanoparticles for Intracellular Drug Delivery.
MCNs
Drug loading Lipids mixing
!
Cell Culture
(a)
(b)
drug
100 nm
5 µm
MCN+doxo.:
16.23 µmole/ doxo./g MCN
MCN+doxo.+lipid:
8.76 µmole/ doxo./g MCN
MCN+doxo. MCN+doxo.+lipid
Texas Red @DHPE
MCN
fluorescence