2. DAILY SPECT QC
• DONE DAILY
• PHOTOPEAK
• UNIFORMITY
• 57Co or 99mTc may be used
• Extrinsic QC done (Collimator Present)
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13. Radiopharmaceutical QC
• To be done daily, prior to administration of the RP
• Must be > 90%
• Methods
• Paper/Instant Thin Layer Chromatography
• HPLC
14. Departmental QC
SL.
No.
Code (TCK) Short form Solvent Rf (RP) Rf (Impurity)
Support: Whatman 3
1 NA NaTcO4 Acetone SF PS
2 5 S/C Acetone (Saline) PS (SF) SF (PS)
3 7 DTPA Acetone (Saline) PS (SF) SF (PS)
4 30 MDP Acetone (Saline) PS (SF) SF (PS)
5 33/35 DMSA Acetone (Saline) PS (SF) SF (PS)
6 39 Mebrofeni
n
MEK (Methanol on ITLC-SG) PS (SF) SF (PS)
7 42 ECD Methanol SF PS
8 43 EC Acetone (H2O: Acetonitrile) PS (SF) SF (PS)
9 50 MIBI Methanol SF PS
10 56 MAA Acetone PS SF
41. HPLC: High Performance Liquid
Chromatography
• Modification of column chromatography
• Small amount of sample to be tested is added (upto 250 mcg)
• UV Spectrometry done before radio-HPLC (at 240 nm)
• Conventional phase solvent: water with 0.1% Tri-Fluoro-Acetic acid
• Reverse phase solvent: Acetonitrile water (22%) with 0.1% Tri-Fluoro-
Acetic acid
• HPLC water with 0.05% NaN3 (sodium Azide): for UV Spectrometry
• Buffer: Phosphate (0.05M) with 0.05% NaN3 at pH 6.8
42. HPLC Continued…
• Stationary phase: High performance silica-based plates
• Process can be automated
• More accurate results than conventional TLC
• Tedious preparation
• Expert knowledge to read the result
47. GFR by Dual Plasma Sampling Method
• Considered the reference method
• Absolute quantification done
• Gold Standard Agent: 51Cr-EDTA – used in Europe: matches kinetics
of Inulin and has very low protein binding
• Cheaper method: using 99mTc-DTPA 100 microCi/taking 2 samples at
1h and 3 hour post GFR study by Gate’s method, then letting it decay
for 24 hours: India
48. GFR by Dual Plasma Sampling Method
Continued
• EDTA-added to blood to prevent coagulation Plasma Separated
Counts taken by Spectrometer
• Done when GFR by Gate’s method is low (usually < 30 ml/min) or
when patient is a potential renal donor
