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Stephen Jenkins

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The research community has begun correlating the makeup of individual microbiomes with disorders and diseases such as obesity, atherosclerosis and cancer. To accomplish this, researchers must first identify and characterize these microbial communities and understand the complex immune interactions between host and pathogen. This webinar provides you with a complete overview of the microbiome, metagenomics and host-pathogen interactions. Experimental strategies, from sample to insight, which can facilitate your microbiology and immunology research, are highlighted.

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High-throughput sequencing, combined with high-resolution metagenomic analysis, provides a powerful diagnostic tool for clinical management of enteric disease. Forty-five patient samples of known and unknown disease etiology and 20 samples from health individuals were subjected to next-generation sequencing. Subsequent metagenomic analysis identified all microorganisms (bacteria, viruses, fungi and parasites) in the samples, including the expected pathogens in the samples of known etiology. Multiple pathogens were detected in the individual samples, providing evidence for polymicrobial infection. Patients were clearly differentiated from healthy individuals based on microorganism abundance and diversity. The speed, accuracy and actionable features of CosmosID bioinformatics and curated GenBook® databases, implemented in the QIAGEN Microbial Genomics Pro Suite, and the functional analysis, leveraging the QIAGEN functional metagenomics workflow, provide a powerful tool contributing to the revolution in clinical diagnostics, prophylactics and therapeutics that is now in progress globally.

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Fusion genes are hybrid genes formed by the fusion of two separate genes. Translocation, interstitial deletion and chromosomal inversions are some of the genetic events that can lead to the formation of fusion genes. The occurrence of fusion genes and its implications in cancer have already been known, but the emergence of NGS technology – especially RNA sequencing – offers the potential to detect novel gene fusions. You can learn more about fusion genes and applying NGS to detect them at our upcoming webinar, presented by Raed Samara, Ph.D., QIAGEN’s Global Product Manager for NGS technologies. In this webinar, Dr. Raed Samara will cover: 1. Fusion genes: what they are and a historical perspective 2. Fusion gene detection: the current status 3. RNA sequencing vs. digital RNA sequencing 4. How to detect and accurately quantify novel fusion genes in your sample

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