Genetic diversity of helicoverpa armigera

2. Vilasrao Deshmukh College of Agril.
Biotechnology, Latur
STUDY OF GENETIC VARIATION ON BASIS OF
FEEDING BEHAVIOR OF BOLLWARM (Helicoverpa
armigera) ATTRIBUTE CADHERIN GENE.
Presented by
Ms. Pakhare Pallavi Eknath
(2018/BT/07/ML)
Research Guide
Dr. A. A. Bharose
Assistant professor
(Department of Plant Biotechnology)

3. CONTENT
 Introduction
 Objectives
 Materials and methods
 References

4. INTRODUCTION
 Helicoverpa armigera have polyphagous nature, with the larvae attacking at
least 60 cultivated and 67 wild host plants from numerous families including
Fabaceae, Malvaceae, Poaceae and Solanaceae (Fitt, 1989, Pogue, 2004).
 Helicoverpa armigera is very devastating pest which cause up to 65% loss to
cotton yields (Ting, 1986).
 The average crop losses in India due to this polyphagous pest are estimated to
be US $350 million annually.
 Feeding behavior on different host is effect on growth and development of
insect.
 Cadherin belongs to one of the families of animal glycoproteins.Cadherin is
also play fundamental roles in development, morphogenesis, cell sorting and
migration and the maintenance of structural integrity (Hulpiau and van Roy).

5. 1. To study the feeding behavior of Helicoverpa armigera on
different field crop.
2. Molecular characterization of Helicoverpa armigera based on
feeding behavior using cadherin specific primer
Objectives

6. 1) Insects
The first and fifth instar larvae of Helicoverpa armigera will collected
from different field crops at Oilseed Research Station, Latur .
2) Leaves of different host plant
Leaves of different host plants will collected from different field crops.
MATERIALS AND METHODS
PLANT MATERIAL
METHODS
1. Feeding assays of Helicoverpa armigera on different host plant:-
observations will recorded during the feeding assays:- mass gained by
the larvae , larval period, percent mortality during first to third, fourth to fifth,
sixth larvae.

7. Cont….
2. Molecular characterization
i. Helicoverpa armigera larvae collected from different
field crop
ii. DNA isolation from larvae
iii. Design specific primer
iv. PCR by using cadherin specific primer

8. REFERENCES
Bernays, E. A. (1994). Evolution of feeding behavior in insect herbivores.
Bioscience 48, 35 –44.
CABI (CAB International) and EPPO (European and Mediterranean Plant
Protection Organization), (1990) . Data sheets on quarantine pests – Helicoverpa
armigera. pp. 6 .
Gujar, G. T., Khawale, R. N.,and Kalia. V, (2007). Genetic variability of
Helicoverpa armigera (Hübner) attributable to cadherin gene-specific molecular
markers, current science, vol. 92, no. 6.
Nagamatsu, Y., Koike, T., Sasaki, K., Yoshimoto, A., Furukawa, Y. (1999). The
cadherin like protein is essential to specificity determination and cytotoxin action
of Bacillus thuringiensis insecticidal cry1Aa toxin.FEBSLett.460, 385-390.
Subramanian, S. and Mohankumar, S. (2006). Genetic variability of the bollworm,
Helicoverpa armigera, occurring on different host plants, Journal of Insect
Science, 6(26): pp.1-8.

9. ADVISORY COMMITTEE
Chairman : Dr. A. A. Bharose
Associate Professor
Department of plant Biotechnology
Vilasrao Deshmukh College of Agril. Biotechnology
Members : Dr.S.R.Bhalarao
Associate Professor
Department of Biochemistry & Molecular Biology
Vilasrao Deshmukh College of Agril. Biotechnology, Latur
Dr. M. S. Dudhare
Associate Professor
Department of Biotechnology
Vilasrao Deshmukh College of Agril Biotechnology, Latur
Dr. D.G. More
Assistant Professor
Department of Entomology,
College of Agricultural, Latur