An undergraduate lecture on role of MHC in antigen presentation including an overview of antigen presentation pathways as well as MHC Class I and II proteins

1. Major Muhammad Omair Riaz
Consultant Immunologist

2. Should be able to:
 Define “Antigen Presenting Cells”
 Describe Major HistocompatibilityComplex
 Explain the role of MHC in antigen
presentation
 And try to keep awake
till the end!

3.  Review of Medical Microbiology &
Immunology, byWarren Levinson
 Chapter 58 & 62
 Immunology made ridiculously simple

5. Which one is. .. . Different?
Does he really belong?

7.  An antigen tries to evade host cell immune
response by:
 Hiding
 Evading detection
 Inhibiting timely response

8. Azad Kashmir, Pakistan

9. Azad Kashmir, Pakistan
Pak Army Snipers
Equipped with RPA
Rangemaster .50 at
LOC on a usual day

11.  “The cells which internalize the antigen and
display it’s antigenic parts on their surface to
be scanned by aT cell”
 These include
 B cells
 Macrophages
 Dendritic Cells

13.  MHC complex (set of genes) situated on
chromosome 6
 It comprises 200 genes with 10000+ alleles
 Product: MHC proteins or HLA antigens
on cell surface
 Types: Class 1 & 2

14.  Within each MHC Class are subtypes
 Class I
▪ A,B,C,E,F,G
 Class 2
▪ D (DP, DQ, DR)
 All subtypes are inherited co-dominantly (one
allele from each parent)
 Everybody has a unique haplotype just like
a blood group
I think
he’s
making it
all up

17.  Talk to your colleague on the left and answer
the following question. Time = 60 seconds
 Google if you have to!
 What are some other applications of MHC
proteins (HLA typing)?

19.  Talk to your colleague on the left and answer
the following question. Time = 60 seconds
 Google if you have to!
 What are some other applications of MHC
proteins (HLA typing)?
 HLA typing for identifying unknown dead bodies
 Paternity testing to determine parentage
 Transplant typing

24. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159193/

28. The quick fire questions!

29.  MHC proteins are expressed on all cells in
human body except:
1. RBCs
2. WBCs
3. Dendritic cells
4. Endothelial cells
5. None of the above

30.  MHC Class I pathway involves antigen
presentation to:
1. CD4 Cells (HelperT cells)
2. CD8 Cells (CytotoxicT cells)
3. CD3 Cells
4. CD19 cells
5. None of the above

31.  Extracellular microbial or other antigens are
processed through which pathway:
1. MHC I
2. MHC II
3. Both
4. None of the above

32.  Professional antigen presenting cells are:
1. Dendritic cells
2. Macrophages
3. B cells
4. T cells
5. None of the above

33.  How many MHC molecules are usually
present on a dendritic cell surface :
1. One
2. Two
3. Four
4. 220,000
5. See, I told you last time, I’m busy whatsapping

35. Download the presentation from
www.slideshare.net/omairriaz