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ESCHERICHIA
coli
diarreicogéni
ca(DEC)
E.Enteropat
ógena
coli(EPEC)
E.Coli
enterotoxig
énica(ETEC)
E.Coli
enteroagregat
iva(CEEA)
E.Coli
enterohem
orrágica(E
HEC)
E.Coli
enteroinvasiva
(EIEC)
Diseases caused by the
consumption of
contaminated water can
affect a large number of
people in a short time, these
sources include human
waste and animal waste
discharged into distribution
systems.
Public water is
managed regularly to
ensure quality,
underground water
that has not been
purified is sometimes
used as drinking water.
School camps have a
variety of distribution
systems, private and
public, are managed
with multi-step
treatment systems or
frequent monitoring.
Factors that contribute
to the microbial
contamination of
drinking water are: low
pressure conditions,
low residual chlorine
content and pipe cuts.
Water
quality
RELATION OF
E.COLI AND
WATER QUALITY
The fecal matter resulting in the contamination
of drinking water, as well as the transmission by
water of enteric pathogens, the elimination of
biological and chemical contaminants from
drinking water before an essential time to ensure
quality.
•The use of groundwater for drinking or cooking
without proper water or sanitation treatment
may increase the risk of infection in people who
consume it.
There have been several outbreaks of water-
borne gastroenteritis caused by DEC, it has been
detected in several ecological niches from
mammalian intestines to various aquatic
environments, such as surface and groundwater.
•An outbreak can be attributed to a single E. coli
clone as the etiologic agent; multiple DEC
strains are involved in a water-based outbreak.
 publicize the
relationship
between infections
associated with
drinking water and
an environment
prone to this as a
school camp.
CASO SOSPECHOSO:
experimentó uno o más
síntomas como: diarrea
(tres o deposiciones más
líquidas por día), dolor
abdominal, vómitos,
náuseas o resfriado.
CASO CONFIRMADO: por
un examen de
laboratorio de un caso
sospechoso con un
infección por E. coli
patógena confirmada
por cultivo.
Todos los asistentes
fueron
cuestionados: los
síntomas clínicos,
consumo de
alimentos y
exposiciones
ambientales
mientras asiste el
campamento y el
momento del
inicio de los
síntomas.
Se brindó
educación sobre
higiene a todos
los participantes
del
campamento,
para controlar los
brotes
transmitidos por
los alimentos o
por el agua.
Electroforesis de gel de campo
pulsado(PFGE): es particular
porque tiene mas electrodos y se
van encendiendo por pulsos.
Fundamento: es una separación
de moléculas por peso molecular
y por carga eléctrica.
Se uso para identificar las
variantes genéticas que habían
entre cepas de E. coli que
estaban en el agua contaminada.
Concentración
Mínima
Inhibidora (CMI)
Fundamento: es
la medida de la
sensibilidad de
una bacteria a
un antibiótico.
Se usa para
evaluar la
concentración
inhibitoria
mínima para el
bicho, cuanto es
lo mínimo que
necesito para
matarlo.
Inspección a las
instalaciones del
campamento,
entrevista al personal
de catering y revisión
de registros de
seguridad alimentaria.
•Como la
cafetería y el
sistema de
suministro de
agua.
Especímenes
ambientales fueron
inicialmente
recolectados de
•Alimentos
servidos,
utensilios de
cocina y agua:
regiones superior
e inferior de un
valle.
Las muestras fueron
probadas, las
concentraciones
residuales de cloro libre
en muestras de agua
bebida fueron
medidas.
•Identificación
bacteriana y
viral
AUTHOR HE SAID YES OR NO
Dutta In humans, symptoms of DEC
infection are associated with
gastrointestinal disease and can
range from mild diarrhoea to severe
abdominal pain and/or cramping,
depending on the pathogenic type
of E. coli involved.
Brooks EHEC infection can cause bloody
diarrhoea and potentially life-
threatening complications, such as
haemolytic uremic syndrome.
Franklin and Gallay A number of waterborne outbreaks
have been associated with the
following potential water system-
related causative factors:
inappropriate cross-connections
between the reservoir and drinking
water pipelines, failure of the
disinfection system, and leaks into
the underground storage tank .
Saxena E. coli is used as a bacterial
indicator of faecal contamination
in water.
A treatment of drinks, food
and aqueducts can
greatly reduce the spread
of both bacterial and viral
infections.
The infection caused by the
different types of
diarrheicogenic E. coli can
evolve gravely and quickly,
therefore, hygiene education
on this subject is essential, thus
significantly reducing its
transmission.
Seminario Biologia Molecular

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Seminario Biologia Molecular

  • 1.
  • 2. ESCHERICHIA coli diarreicogéni ca(DEC) E.Enteropat ógena coli(EPEC) E.Coli enterotoxig énica(ETEC) E.Coli enteroagregat iva(CEEA) E.Coli enterohem orrágica(E HEC) E.Coli enteroinvasiva (EIEC) Diseases caused by the consumption of contaminated water can affect a large number of people in a short time, these sources include human waste and animal waste discharged into distribution systems. Public water is managed regularly to ensure quality, underground water that has not been purified is sometimes used as drinking water. School camps have a variety of distribution systems, private and public, are managed with multi-step treatment systems or frequent monitoring. Factors that contribute to the microbial contamination of drinking water are: low pressure conditions, low residual chlorine content and pipe cuts. Water quality
  • 3. RELATION OF E.COLI AND WATER QUALITY The fecal matter resulting in the contamination of drinking water, as well as the transmission by water of enteric pathogens, the elimination of biological and chemical contaminants from drinking water before an essential time to ensure quality. •The use of groundwater for drinking or cooking without proper water or sanitation treatment may increase the risk of infection in people who consume it. There have been several outbreaks of water- borne gastroenteritis caused by DEC, it has been detected in several ecological niches from mammalian intestines to various aquatic environments, such as surface and groundwater. •An outbreak can be attributed to a single E. coli clone as the etiologic agent; multiple DEC strains are involved in a water-based outbreak.
  • 4.  publicize the relationship between infections associated with drinking water and an environment prone to this as a school camp.
  • 5. CASO SOSPECHOSO: experimentó uno o más síntomas como: diarrea (tres o deposiciones más líquidas por día), dolor abdominal, vómitos, náuseas o resfriado. CASO CONFIRMADO: por un examen de laboratorio de un caso sospechoso con un infección por E. coli patógena confirmada por cultivo. Todos los asistentes fueron cuestionados: los síntomas clínicos, consumo de alimentos y exposiciones ambientales mientras asiste el campamento y el momento del inicio de los síntomas. Se brindó educación sobre higiene a todos los participantes del campamento, para controlar los brotes transmitidos por los alimentos o por el agua.
  • 6. Electroforesis de gel de campo pulsado(PFGE): es particular porque tiene mas electrodos y se van encendiendo por pulsos. Fundamento: es una separación de moléculas por peso molecular y por carga eléctrica. Se uso para identificar las variantes genéticas que habían entre cepas de E. coli que estaban en el agua contaminada.
  • 7. Concentración Mínima Inhibidora (CMI) Fundamento: es la medida de la sensibilidad de una bacteria a un antibiótico. Se usa para evaluar la concentración inhibitoria mínima para el bicho, cuanto es lo mínimo que necesito para matarlo.
  • 8. Inspección a las instalaciones del campamento, entrevista al personal de catering y revisión de registros de seguridad alimentaria. •Como la cafetería y el sistema de suministro de agua. Especímenes ambientales fueron inicialmente recolectados de •Alimentos servidos, utensilios de cocina y agua: regiones superior e inferior de un valle. Las muestras fueron probadas, las concentraciones residuales de cloro libre en muestras de agua bebida fueron medidas. •Identificación bacteriana y viral
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  • 13. AUTHOR HE SAID YES OR NO Dutta In humans, symptoms of DEC infection are associated with gastrointestinal disease and can range from mild diarrhoea to severe abdominal pain and/or cramping, depending on the pathogenic type of E. coli involved. Brooks EHEC infection can cause bloody diarrhoea and potentially life- threatening complications, such as haemolytic uremic syndrome. Franklin and Gallay A number of waterborne outbreaks have been associated with the following potential water system- related causative factors: inappropriate cross-connections between the reservoir and drinking water pipelines, failure of the disinfection system, and leaks into the underground storage tank . Saxena E. coli is used as a bacterial indicator of faecal contamination in water.
  • 14. A treatment of drinks, food and aqueducts can greatly reduce the spread of both bacterial and viral infections. The infection caused by the different types of diarrheicogenic E. coli can evolve gravely and quickly, therefore, hygiene education on this subject is essential, thus significantly reducing its transmission.