This document describes the materials and methods used in an experiment involving anaerobic digestion in chambers. Wastewater samples with higher solids content and glycerol co-substrates from farm and industrial sources were used as feed for inoculum sludge in sealed digesters. Biogas volume was measured using gas collectors connected to the digesters. Chemical oxygen demand, gas sampling, and chromatographic analysis of output peaks were used to analyze feed, effluent, and biogas composition.