1. Leon Oosterik May 2015 1
Leon Oosterik
Personalia
Address: Kanaaldijk 1
7471 SG Goor
Date of birth: 09-03-1987
Mobile: +31 630 79 69 12
Gender: M
Nationality: Dutch
E-mail address: leon.oosterik@gmail.com
Driving license: B (Dutch)
Work experience
2011 - 2014 PhD in Bioscience Engineering Leuven/Brussels (BEL)
KU Leuven and CODA-CERVA
Organizing practical immunology courses for Master’s students
Education
2011 - 2014 PhD in Bioscience Engineering Leuven/Brussels (BEL)
KU Leuven and CODA-CERVA
The potential of disinfectants and bacteriophages to
reduce avian colibacillosis
Promotor: prof. Bruno Goddeeris (KU Leuven)
Co-promotor: prof. Patrick Butaye (CODA-CERVA)
Scholarship: • Feb 2011 - Jan 2013: Federal Public Service
Health, Food Chain Safety and Environment
• Feb 2013 - Jul 2014: CODA-CERVA
Date of graduation: Oct 2014
2008 - 2010 Master in Animal Sciences Wageningen (NLD)
Specialisation: Animal Health and Behaviour
Wageningen Universiteit
Major thesis: Natural antibodies as parameters for mastitis
resistance in dairy cows
Minor thesis: Development of a chikungunya subunit vaccine;
Exchange of the native signal sequence of E1 and E2
glycoproteins for the Honeybee Melittin signal sequence
Internship: Detection of Escherichia coli infection Edmonton (CAN)
of the liver in newly hatched chicks by entering the navel
University of Alberta

2. Leon Oosterik May 2015 2
2005 - 2008 Bachelor in Animal Sciences Wageningen (NLD)
Minor in biology of infectious diseases of humans and animals
Wageningen Universiteit
Bachelor thesis: Culture of avian embryos in
semi-shell-less and shell-less culture
Technical skills
Antibiotic/disinfectant susceptibility testing, bacterial/phage isolation,
bacterial transformation, biofilm formation assay, cloning, DNA purification,
gel electrophoresis, immunohistochemistry, infection studies, ligation, PCR,
phage amplification, restriction enzyme digestion, sequencing
Extra courses
Laboratory Animal Science (Article 9 certified)
Languages
Dutch: native
English: good
German: basic
IT/computer skills
General: Microsoft Word, Excel and PowerPoint
Specialized: FastPCR, Chromas, CLC Sequence Viewer
Extracurricular activities
2006 - 2008 Secretary student swimming association 'Aquifer' Wageningen (NLD)
Hobby’s
Mountain biking, running, swimming, travelling
References
References upon request

3. Leon Oosterik May 2015 3
Publications
Tsonos*, J., Oosterik*, L.H., Tuntufye, H.N., Klumpp, J., Butaye, P., De Greve, H., Hernalsteens, J.-
P., Lavigne, R., Goddeeris, B.M. 2014. A cocktail of in vitro efficient phages is not a guarantee for in
vivo therapeutic results against avian colibacillosis. Veterinary Microbiology 171 (3-4), 470-479.
* shared first authorship
Oosterik, L.H., Peeters, L., Mutuku, I., Goddeeris, B.M., Butaye, P. 2014. Susceptibility of avian
pathogenic Escherichia coli from laying hens in Belgium to antibiotics and disinfectants and
integron prevalence. Avian Diseases 58 (2), 271-278.
Oosterik, L.H., Tuntufye, H.N., Butaye, P., Goddeeris, B.M. 2014. Effect of serogroup, surface material
and disinfectant on biofilm formation by avian pathogenic Escherichia coli. The Veterinary Journal
202, 561-565.
Presentations
Oosterik, L.H., Peeters, L., Butaye, P. Susceptibility of avian pathogenic Escherichia coli
from laying hens in Belgium to antibiotics and disinfectants and integron prevalence. National
Symposium on Applied Biological Sciences. Gembloux, Belgium. September 07, 2014. Poster.
Tsonos*, J., Oosterik*, L.H., Tuntufye, H.N., Butaye, P., De Greve, H., Hernalsteens, J-P, Lavigne, R.,
Goddeeris, B.M. A cocktail of in vitro efficient phages is not a guarantee for in vivo therapeutic results
against avian colibacillosis. Belgian Society for Microbiology Annual Symposium. Brussels, Belgium.
November 26-27, 2013. Poster
Tsonos*, J., Oosterik*, L.H., Tuntufye, H.N., De Greve, H., Lavigne, R., Hernalsteens, J-P, Goddeeris,
B.M. Treatment of experimentally infected chickens with a bacteriophage cocktail. 10th
International
Veterinary Immunology Symposium. Milan, Italy. Augustus 28-September 01, 2013. Poster
Tsonos, J., Oosterik, L.H. , Klumpp, J., Willems, J., Goddeeris, B.M., De Greve, H., Lavigne, R.,
Hernalsteens , J-P. Bacteriophages as an alternative way to treat and prevent APEC infections. Viruses
of Microbes. Brussels, Belgium. July 16-20, 2012. Poster
* shared first authorship
Conferences
Escherichia coli and the Mucosal Immune System: Interaction, Modulation and Vaccination.
Ghent, Belgium. July 2-5, 2011.