This document summarizes the automation of Omega Bio-tek's Mag-Bind Plasmid DNA extraction kit on the Hamilton STAR liquid handling robot. The system uses magnetic beads to purify plasmid DNA from bacterial cultures in a 96-well format. The STAR robot performs the full plasmid extraction procedure, including resuspension, lysis, binding to magnetic beads, washing, and elution over four automated phases. This allows processing of up to 48 plates in 9 hours with minimal user intervention and yields high quality plasmid DNA suitable for downstream applications like sequencing and transformation.

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Automated Plasmid DNA Extraction Using Omega Bio‐tek’s
Mag‐Bind® Plasmid DNA Kit on the Hamilton STAR
Omega Bio-tek’s Mag-Bind®
Plasmid DNA extraction kit is a magnetic beads-based plasmid DNA
purification system that uses a single protocol to purify variety of high and low copy of plasmid, BACs,
PACs, and cosmids. This flexible system has been automated on Hamilton’s STAR without sacrificing
plasmid DNA quality over traditional filter-based extraction methods.
Features/Benefits:
• Process up to 48 plates in 9 hours
• Minimal user intervention
• High copy plasmid yields up to 10 µg per 1.5 mL culture (2XLB/Amp) in a 96 well
plates ideally formatted for downstream applications including PCR±
, Sanger sequencing,
transformation, etc.
Four pre-written protocols are available for the STAR instrument including:
• Phase 1, Resuspension, Lysis, & Neutralization
• Phase 2, Bead Binding to Cleared-Lysate
• Phase 3, Wash & Elute
• Phase 4, Transfer Purified DNA to New Plate
The Mag-Bind module automates:
• Resuspension, lysis, & neutralization.
• Magnetic bead capture of plasmid DNA
• Magnetic bead washing & elution
• Transferring of purified DNA to final plate

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STAR deck with the components of the Mag-Bind System Phase 1
STAR deck with the components of the Mag-Bind System Phase 2
STAR deck with the components of the Mag-Bind System Phases 3 & 4

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Ordering information
Hardware Required Part Number
MICROLAB STAR Workstation with 96 channel head and all necessary
carriers and accessories
Magnet Carriers
Centrifuge capable of holding 4 2.2mL deep well blocks generating 3200 RFGs
???
Various Options available
Various Options available
Consumables Required
Hamilton 300ul CO-RE disposable tips, frame racks
Mag-Bind Plasmid 96 Kit
300uL Reagent Troughs
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M1256-01, M1256-02
Various Options available
More information can be found at: Omega Bio-tek Mag-Bind Plasmid DNA 96
±
PCR is a patented process of Hoffman-La Roche. Use of the PCR process requires a license.
For life science research only. Not for use in diagnostic procedures.
For more information, please visit www.hamiltonrobotics.com or contact regional sales manager.
Method validation and testing performed by iXpressGenes, Inc at the HudsonAlpha institute for Biotechnology.