1.  References:
• The Mechanism of Action of Calcium in the Inhibition of High Temperature-Induced
Leakage of Betacyanin From Beet Root Discs- A.Cooke, A.Cookson and M.J Earnshaw 1986
• Plasma Membrane of Beta vulgaris Storage Root Shows High Water Channel Activity
Regulated by Cytoplasmic pH and Dual Range of Calcium Concentrations- Alleva, Karina
2006
• Aluminum interactions with K+ (86RB+) and 45Ca2+ Fluxes in Three Cultivars of Sugar Beet
(Beta Vulgaris)- Lindberg S- 1990

2. 0.5 Our results did not
0.45
indicate a trend, but
Based on our
0.4
references we know
0.35
that calcium does
Absorbancey
0.3
inhibit betacyanin
(525 nm)
0.25 leakage.
0.2
0.15 The error bars show
0.1 how the trials within
0.05
each concentration
varied compared to the
0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 average.
Concentration (mol/L)
Control Value: 0.267 0.511M : 0.291
0.106 M : 0.382 0.822 M : 0.236
0.200 M : 0.227