6. A Novel Teaching-Learning-Based
Optimization for Improved Mutagenic Primer
Designin Mismatch PCR-RFLP SNP
Genotyping.
In order to improve the
mutagenic primer design,
they propose TLBOMPD
(TLBO-based Mutagenic
Primer Design) a novel
computational intelligence-
based method that uses
the notion of "teaching and
learning" to search for
more feasible mutagenic
primers and provide the
latest available restriction
enzymes.
7. A Novel Teaching-Learning-Based
Optimization for Improved Mutagenic Primer
Designin Mismatch PCR-RFLP SNP
Genotyping.
Mutagenic matrix is
also reserved to
increase the efficiency
of judging a
hypothetical
mutagenic primer if
involve available
restriction enzymes for
recognizing the target
SNP.
8. A Novel Teaching-Learning-Based
Optimization for Improved Mutagenic Primer
Designin Mismatch PCR-RFLP SNP
Genotyping
Furthermore, the
core of SNP-
RFLPing version
2 is used to
enhance the
mining work for
restriction
enzymes based
on the latest
REBASE.
The TLBOMPD is
implemented in JAVA
and it is freely available
at
http://tlbompd.googleco
de.com/.
9. A Novel Teaching-Learning-Based
Optimization for Improved Mutagenic Primer
Designin Mismatch PCR-RFLP SNP
Genotyping
Student´s observation:
I think this investigation shows us the importance of
techniques discussed in class, in this case we can see how
restriction enzymes help to find mutagenic primers
11. Mechanism of extraordinary DNA digestion
by pepsin
. The digestion was specific (sequence
dependent to some extent), and pepsin
preferred to cleave purine-rich sequences.
The digestion of favorable sequence was
dramatically accelerated when the purine
base at the cleavage site was removed
(created an apurinic (AP) site).
12. Mechanism of extraordinary DNA digestion
by pepsin
The results indicated that pepsin
preferred to digest longer DNA (e.g.
> 59 bases) than shorter one, and
sequence shorter than 30 bases
was barely digested.
13. Mechanism of extraordinary DNA digestion
by pepsin
However, the AP site
did not help to cleave
the sequence that
pepsin could not
cleave originally
14. Mechanism of extraordinary DNA digestion
by pepsin
Student´s observation:
I think this study is important beacuse they
demonstrated that pepsin has activities similar to
endonucleasses and i think it may be useful for
future medical research.
