In Figure 4- the target DNA used for a genetic study was digested with (2).pdf

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In Figure 4 , the target DNA used for a genetic study was digested with restriction enzyme enzymes Eco RI and Bam H1 in various reaction tubes. The samples were then electrophoresed and stained with a fluorescence dye. Carefully review the diagrams and respond to this question: The number of fluorescence-stained bands visible in the double digest ( Eco RI/ Bam HI) in Figure 4 is________ Figure 4 - Fluorescent Stained Gel.

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In 2020, the world was made aware of the highly contagious and occasionally fatal communicable disease known as Covid-19, in March of that down, this SARS-CoV-2 virus and its spread led to a shutdown of most of society. Children stayed home from school, and adults tried to work remotely if they could. Restaurants served food outdoors or through take-out only or shut their doors permanently. Movie theatres closed, and sporting events had no fans in the stands. Community centers and even houses of worship could not function as they typically do. Patients in hospitals or elders in nursing homes could no longer have family members visit them. If you did go anywhere, you had to wear a mask covering your nose and mouth, limiting your ability to express or read others emotions. Overall, it was socially isolating During the first few weeks, some families enjoyed spending more time together, but even the closest of families soon tired of the new homebound routine. For this assignment. you are asked to include details of your memories of those first few weeks of Covid isolation in 3 well-developed paragraphis. Describe the changes to your daily life and the emotional costs of isolation. - Describe your first encounter with CoVid-19 - Where were you? - How did you learn about it? - Another person - Mainstream Media - news reports on TV, radio, or paper - Social Media - What were your first impressions? - Do you think how you leamed of the virus influenced your first impressions? - How long did you expect your life to be disrupted? - In the following weeks - How did your city, town, county, or state respond to the virus? o How did your family cope with the challenges of CoVid isolation? - What was the most difficult thing you had to adjust to during enforced social isolation? - Did you or anyone you know contract the virus? If so, did you or they have to be hospitalized? What was that experience like? - In hindsight- - Is there anything you believed in Aprii 2020 that you no longer believe about the CoVid-19 pandemic? - How would you have changed your response to the threat of CoVid-19? - How did your experience with Covid-19 change the way you live? - How did it change the way you feel about communicable diseases?.

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In 2019, a dam built to hold waste from a nearby iron ore mine owned by the Vale Mining Company (Vale) in Brumadinho, Brazil, collapsed, killing 270 people. The tsunami of mud was so powerful that it overturned houses, mangled trucks, and ripped off the scalps of some of the people buried under the toxic mix of water and mining waste. It also caused vast environmental damage. After prosecutors investigating the disaster found evidence that safety reports were fabricated, they charged 16 officials of Vale, including Vale's chief executive, Fabio Schvartsman, with homicide. Five people at TUV SUD, the German auditing company that certified the minewaste dam as safe months before it gave way, were also charged with homicide. A report issued by an independent commission hired by Vale attested that Vale executives knew about the safety risks at the dam for over a decade and had received multiple warnings, especially in 2015 after a similar dam partly owned by Vale burst, leaving 19 dead. Investigators characterized the relationship between Vale and TUV SUD as one of "pressure, collusion, compensation, and a conflict of interests." Makoto Namba, a senior engineering inspector at TUV SUD, found evidence indicating potentially risky conditions at the dam, saying, "Everything suggests (the dam) won't pass a key safety test." Namba later told police that he felt pressured by a Vale official to sign a safety certificate and worried that failing to sign the certificate would jeopardize his career. Without the signed certificate, Vale would have had to halt its adjacent mining operations, which were estimated to produce more than $1 million worth of iron ore a day. Hundreds of people could have lost their jobs, and the report would have triggered a mass evacuation of local communities. TUV SUD certified the dam's safety six months before the collapse and again three months later, after another auditing and certification company, fired by Vale, had warned that the dam was structurally unsound. TUV SUD employees expressed worry about losing contracts with Vale, its major client, if their reports did not verify the dam's safety. Olavo Coelho, a 63-year-old long-time Vale employee, told reporters he worried about the safety of a dam and pointed out cracks, leaks, and dark stains in the structure to Vale engineers. He urged Vale executives to evacuate the suite. "He was ignored," said Coelho's daughter. Wilson Jose Ferreira, a 55-year-old machine operator, said he tried to warn his bosses about the growing number of leaks in the dam. "They paid little attention to us, they didn't believe what we were saying." Several other mine workers warned their bosses at Vale that the dam was about to collapse. Supervisors reportedly brushed aside these concerns, citing fears about extra expenditures that could cost the company more than $1 , 000 to fix the foundation. A Vale spokesperson said the company allowed employees to file anonymous complaints but did not receive m.

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In 1998-99 John Leonard watched a Pepsi commercial and thought he had an opportunity to purchase a Harrier Jet (military aircraft) worth approximately $23M for the cash equivalent of $700K. He collected enough money, added the jet to the bottom of the order form in the Pepsi catalog and sent it in to Pepsi. When they refused to produce the jet and returned his money, he filed a lawsuit for breach of contract. He claimed that the Pepsi Commercial was a valid offer to make and contact and he accepted that offer by sending in the money. After watching the video presentation of Pepsi's Harrier Jet Commercial, (Links to an external site.) discuss the following questions completely using contract law principles. 1. How does the objective theory of contracts apply to this case? (The objective theory is explained on pages 189-190 of your textbook. This rule states that unless a third party looking at the situation objectively thinks that both the offeror and offeree want to enter into a contract, no contract can be created. Offers or statements made in jest , anger or undue excitement do not include the necessary intent to be valid offers. Did Pepsi intend to make an offer in this case?) 2. How does the "advertisement as a special offer" rule apply to this case? (This rule is found on page 204 of the textbook. It says that a commercial or advertisement for the sale of goods - like the Harrier Jet - is not offers to make a contract, even if a specific price for the good is listed by the seller.) 3. The court concluded that no contract existed between John Leonard and Pepsi? Do you agree? Beside to support whatever position you take using contract law principles. For another perspective on this case, you may want to watch this video from things You Might Not Know.

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