This study aimed to analyze the in vitro diagnostics market in Kolkata, specifically for HbA1c testing. The researcher surveyed hospitals, diagnostic centers, and laboratories to understand market size, methods used, and customer needs. Key findings include: (1) The estimated annual market size for HbA1c testing in Kolkata is Rs. 6 crores. (2) HPLC is the dominant testing method, used by 67% of customers. (3) Customers prefer lower cost tests and the average current cost is Rs. 121.93 per test.