Green synthesis of silver nanoparticles

1. School of Health and Allied Sciences
Pokhara University
SYNTHESIS OF PLANT-MEDIATED SILVER NANOPARTICLES
USING FICUS MICROCARPA LEAF EXTRACT AND
EVALUATION OF THEIR ANTIBACTERIAL ACTIVITIES
Biswash Sapkota
Sixth Semester, B. Pharm
School of Health and Allied Sciences
Pokhara University, Lekhnath-12, Kaski, NEPAL
6/5/2021 1

2. School of Health and Allied Sciences
Pokhara University
Background
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Fig: Ficus Microcarpa
• Pantropical genus
• Hypanthodium fruits
•Alternate, simple,oval- elliptic
•Sterols, triterpenoids,
flavonoids, phenol and tannin

3. School of Health and Allied Sciences
Pokhara University
Contd..
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Fig: Nanoparticles
1- 100nm
 Area of
scientific
research
Bio-medical,
Electric, Optical
etc.

4. School of Health and Allied Sciences
Pokhara University
Introduction
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Nano particles of
silver
Composed of silver
oxide
Spherical, diamond ,
octagonal etc
Biological methods
antibacterial,
antifungal, anti-viral and
anti-inflammatory
Fig: Silver nanoparticles

5. School of Health and Allied Sciences
Pokhara University
Materials
oLeaves
oDistilled water
oSilver Nitrates
oWhatman No-1 filter paper
oUV-Visible spectroscopy
oFT-IR
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6. School of Health and Allied Sciences
Pokhara University
Methods
1. Preparation of Leaves extracts
Fresh leaves
Washed, Chopped, Dried
10gm , 100ml distilled water, 20Min boiling
Filtered, Clear solution, Refrigerated
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7. School of Health and Allied Sciences
Pokhara University
2. Synthesis of AG NPs
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Fig: Biological Method
• Extract+ AgNO3
(1:8)
• 24hours at room
temperature
•Color changed is
observed
•Colloidal particle
•Characterization

8. School of Health and Allied Sciences
Pokhara University
3. UV-Vis Spectra Analysis
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Fig: Instrumentation of UV-Vis Spectroscopy
1ml suspension
2ml distilled water
200-700nm

9. School of Health and Allied Sciences
Pokhara University
4. FTIR Analysis
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Fig: FTIR instrumentation
Dried Ag NPs
KBr pellet method

10. School of Health and Allied Sciences
Pokhara University
5. Estimation of Anti-bacterial Activity
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Bacillus cereus, Escherichia coli, Klebsiella
pneumonia,and Staphylococcus aureus
Well Diffusion Method
10, 20,30,40 μl/well
Gentamicin(10μg)(+ve)control
Zone of inhibition

11. School of Health and Allied Sciences
Pokhara University
Results and Discussions
1. Synthesis of Ag NPs
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Fig: A: Solution of AGNO3
B: Solution of Extract
C: Plant extract with AgNO3
Yellow- brown color
Reddish brown color
Colloidal brown
Stable Ag NPS
Fig: Synthesized Ag NPS

12. School of Health and Allied Sciences
Pokhara University
2. UV-Vis Spectroscopy Analysis
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Fig: Absorption spectra of Ag NPs Synthesized
451nm

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Pokhara University
3. FTIR Spectroscopy Analysis
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Fig : Ag NPs FTIR spectra
Band at
3474cm-: O-H stretching
1638cm-:H bonded alcohols
1455cm-: carbonyl stretching
1079 cm-:N-H bond 1o amine

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Pokhara University
4. Antibacterial Activity
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A: Bacillus cereus(+ve)
B: Escherichia coli(-Ve)
C: Klebsiella pneumonia(-ve)
D: Staphylococcus aureus(+ve)
40 μl/well 10 μg

15. School of Health and Allied Sciences
Pokhara University
Conclusions
•Ag Nps exhibit a broad size distribution and morphologies
with highly reactive activity.
•The major mechanism through which Ag NPs manifested
antibacterial properties is by attaching to and penetrating
the bacterial cell wall, and modulating cellular signalling.
•However, further studies must be conducted to verify if
the bacteria develop resistance towards the nanoparticles
and to examine cytotoxicity of a nanoparticles towards
human cells before proposing their therapeutic use.
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16. School of Health and Allied Sciences
Pokhara University
Reference
Praba, P. S., Jeyasundari, J., Brightson, Y., & Jacob, A. (2015). SYNTHESIS OF PLANT-
MEDIATED SILVER NANOPARTICLES USING FICUS MICROCARPA LEAF
EXTRACT AND EVALUATION OF THEIR ANTIBACTERIAL ACTIVITIES. European
Chemical Bulletin, 4 (3), 116-1120.
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17. School of Health and Allied Sciences
Pokhara University
Thank you
Goods things come in small package
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