Biopharmaceutic • It is the science that examined the interrelationship between physicochemical properties and the dosage form in which the drug is given , route of administration and its affect on the rate and extent of systemic drug absorption , metabolism and excretion

1. Biopharmaceutics
4th Year
Introduction to the Biopharmaceuics
Lab 1
Assistant Lecturer Ahmad.A.Yosef
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2. Introduction
• Biopharmaceutic
• It is the science that examined the interrelationship between
physicochemical properties and the dosage form in which the drug is
given , route of administration and its affect on the rate and extent of
systemic drug absorption , metabolism and excretion
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3. Introduction
Biopharmaceutic involves many factors that influence:
(1) The design of the drug product
(2) Stability of the drug within the drug product
(3) The manufacture of the drug product
(4) The release of the drug from the drug product
(5) The rate of dissolution/release of the drug at the absorption site
(6) Delivery of drug to the site of action
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5. Types of Biopharmaceutics
• Biopharmaceutic is divided into
1. Pharmacodynamics
2. Pharmacokinetics
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6. Pharmacodynamics
Definition
In Greek Pharmacon = Drug and Dynamics = Action/Power
It covers all the aspects relating to “What a drug does to the body” Mechanism of
action , it is the study of the biochemical and physiological effects of drugs on the
body; this includes the
•Mechanisms of drug action
•Relationship between drug concentration and effect.
A typical example of pharmacodynamic is how a drug interacts quantitatively with
a drug receptor to produce a response (effect) 10/30/2018 6

7. Pharmacokinetics
• Pharmacokinetics
• It is how the body acts on the drug- involves the study of absorption,
distribution, metabolism (biotransformation) and drug excretion
• Movement of drugs in the body
• Four Processes
• Absorption
• Distribution
• Metabolism
• Excretion
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8. Pharmacokinetics
• Drug concentration at sites of action is influenced by several factors, such as:
1. Route of administration
2. Dose
3. Characteristics of drug molecules (e.g., lipid solubility)
• The experimental aspect of pharmacokinetics involve 3 steps
• 1. The development of biologic sampling techniques
• 2. Analytical methods for the measurement of drugs and metabolites
• 3. Procedures that facilitate data collection and manipulation
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9. Calibration curve
Calibration curve
It is the curve prepared from a series of standard solution to use it as
a reference curve to obtain the concentration of unknown sample for the same
drug.
Standard solution is a dilute solution of known concentration which is
prepared from stock solution using dilution equation
C1.V1=C2.V2
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10. Calibration curve
• Stock solution is a solution of unknown and high concentration from
which a standard solution is prepared
• Blank solution is a solution that contains all the constituents of the
sample except the active ingredient which is required to be measured
• Spectrophotometery method that used to measure the concentration
depends on the light that absorbed
• Lama Max (λ) it’s the wavelength at which the maximum absorption
occurs and measured by (nm)
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11. Calibration curve
We have two types of instrument that depend on applying certain waves
• 1. Visible spectrophotometer (V) 400-700 nm for colored solution
• 2.Ultraviolet spectrophotometer (UV) 200-400 nm
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12. Calibration curve
• There are two methods to draw the calibration curve
1. Eye fitting method ( Not reliable ) which involves drawing straight line among
the scattered points
2. Last square fitting ( More reliable ) this method minimizes the sum of square
deviation of observed value from the line
∑= (y-ŷ)2
Straight line equation Y=C+bX
Y = calculated value, X=concentration , C=Intercept and b=slop
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