1. Chapter 8, objective # 17
List the antigens that are denatured by
routine blood bank enzymes and
antigens whose reactivity with antibody
is enhanced with enzymes?

2. Antigens denatured activity by
enzymes
M and N antigens Easily destroyed by the routine blood
bank enzymes ficin, papain, and
bromelin.
Less common enzymes are trypsin and
pronase.
S and s antigen Less easily degraded by Ficin, papain,
bromelin, pronase, and chymotrypsin
can destroy S and s activity, but the
amount of degradation may depend on
the strength of the enzyme solution.

3. Fya and Fyb antigen Antigens are destroyed by proteolytic
enzymes such as ficin, papain, bromelin,
and chymotrypsin, and by ZZAP.
Neuramidase may reduce the molecular
weight of Fya and Fyb antigen, but it does
not destroy antigenic activity and neither
does purified trypsin.

4. Antigens enhanced activity by
enzymes
I and i antigen Treatment of RBCs with ficin and papain
enhances reactivity of I and I antigens
with their respective antibodies.
The I and i antigens are resistant to
treatment with DTT and glycine- acid
EDTA.
P1 It is resistant to different enzymes
treatment.
Reactivity of the antibodies can be
greatly enhanced by testing with
enzyme-treated RBCs.

5. Jka and Jk b antigen Kidd antigens are not denatured by
papain or ficin; treatment of RBCs with
enzymes generally enhances reactivity
with Kidd antibodies.