More Related Content Similar to LLTECH LIGHT-CT SCANNER IMAGE ATLAS (20) LLTECH LIGHT-CT SCANNER IMAGE ATLAS2. TABLE
OF
CONTENTS
• BREAST
Page
10
• BRAIN
Page
15
• SKIN
Page
21
• LUNG
Page
31
• KIDNEY
Page
35
• EYE
Page
39
• NEEDLE
CORE
Page
45
• EMBRYOLOGY
Page
48
• PLANTS
Page
56
© LLTECH 2011 2
3. The
Light-‐CT
scanner
allows
fast
>ssue
processing
and
pathology
examina>on.
It
completes
the
toolset
available
to
pathologists
Tissue
Scanner
3D
Biopsy
Digital
Image
5-‐8
minutes
Non-‐DestrucIve
2D
Digital
Image
Slide
15-‐12
mins
When
Frozen
SecIon
is
needed
Artefacts
(Mostly
Intra-‐operaIve)
DestrucIve
12-‐24
hours
When
Chemical
FixaIon
is
needed
Expensive
(Mostly
DiagnosIc)
DestrucIve
© LLTECH 2011 3
4. Imaging
in
sca]ering
media:
techniques
u LightCT
© LLTECH 2011 ©
LLTECH
2012
–
www.lltechimaging.com
4
5. Light-‐CT
scanner
OpIcal
acquisiIon
unit,
• User
friendly
moving
verIcally
acquisiIon
soaware
• DICOM
2D
Movable
tray
and
3D
with
sample
Viewer
holder
X,Y
moving
stage
White
Light
JoysIck
for
easy
Integrated
wide
field
camera
to
Source
control
of
X,Y,
Z
take
sample
picture
before
movements
imaging
© LLTECH 2011 ©
LLTECH
2012
–
www.lltechimaging.com
5
6. Light-‐CT™
key
benefits
• OpIcal
in-‐depth
biopsies
of
gross
Issue
within
minutes
• 1
µm
2D
and
3D
histopathological
resoluIon
• Easy
exploraIon,
acquisiIon
and
rendering
in
DICOM
format
• Safe,
non-‐invasive
and
non-‐destrucIve
process
Fast
and
non-‐invasive
3D
in-‐depth
structural
and
cellular
imaging
7. Light
CT
technology
Based
on
Full-‐Field
OpIcal
Coherence
Tomography
(FFOCT)
Combines
microscope
resoluIon
with
interferometry
High
resoluIon
in-‐depth
C
scans
Commercial
device
specs:
• Excellent
resoluIon:
1.5µm
transverse,
1µm
axial
• 70Hz
max.
tomographic
frame
rate
–
0.8
mm
x
0.8
mm
• PenetraIon
depth
200µm
–
1mm
depending
on
Issue
sca]ering
• 25
mm
diameter
sample
size
• Small
footprint:
Scanner
and
light
source
fit
on
70
cm
x
35
cm
8. Image
acquisiIon
1.Prepare
2.
Select
area
to
be
3.
AutomaIc
4.
Analyze
result
in
sample
scanned
scanning
the
viewer
§ Put
sample
• The
system
• The
user
selects
the
• Results
are
analyzed
in
the
into
holder
takes
a
wide
area
to
be
scanned
DICOM
viewer
§ Put
sample
field
picture
from
the
wide
field
• 2D
and
3D
rendering
holder
into
image
• DICOM
image
database
tray
• The
user
launches
the
• Export
to
various
formats:
automaIc
scanning
DICOM,
TIF,
JPEG,
GIF…
• MulIple
languages
for
image
viewer
(English,
Fast
and
easy
process:
1
cm2
in
5-‐7
minutes
Arabic,
French…)
© LLTECH 2011 8
11. Healthy
breast
Issue
Assayag
et
al,
TCRT
Express
2013
in
press
Grainy
aspect
of
normal
Duct
with
Lobule
Adipocytes
Vessel
fibrous
Issue
calcificaIon
©
LLTech
2012
© LLTECH 2011 Courtesy
of
Hôpital
Tenon,
Paris,
France
11
12. INVASIVE
ADENOCARCINOMA
:
NODULAR
TUMOR
Interface
of
the
tumourous
and
normal
appearing
part
:
Different
aspect
of
the
fibrous
>ssue
Tumour
margin
500µm
Highly
scaHering
thin
trabeculae
aspect:
fibrous
>ssue
in
the
tumourous
part
100µm
Grey
zones
corresponding
to
Grainy
foci
of
carcinomatous
cells
medium
scaHering
aspect:
healthy
500µm
SENSITIVITY=
97%
fibrous
>ssue
©
LLTECH
2011
© LLTECH 2011 SPECIFICITY=74%
12
13. Normal
Sen>nel
Node
Adipocytes
in
hilum
Nodules
containing
lymphoid
>ssue
© LLTECH 2011 Capsule
13
14. SENSITIVITY=
100%
Invaded
Sen>nel
Node
SPECIFICITY=97%
Invaded
zone
Lymphoid
zone
Bright
white
aspect,
highly
scaHering
dense
collagen
meshwork
Grieve
et
al
Proc
SPIE
2013
© LLTECH 2011 14
16. FF-‐OCT
imaging
of
human
epilep>c
brain
and
cerebellum
iden>fies
myelinated
axon
fibers,
a
subpopula>on
of
neuronal
cell
bodies
and
CNS
vasculature.
Cortex
Neuron
cell
bodies
(dark
dots)
Axon
bundles
Axons/myelin
fibers
Capillary
White
maHer
Harms
et
al
Proc
2011 2013
© LLTECH SPIE
16
17. Hippocampus:
Coronal
sec>on
(led
side)
Sub-‐
Ependymal
zone
Pyramidal
neurons
of
the
CA4
field
responsible
for
memory
CA4
field
Alveus
Stratum
radiatus
Hippocampal
sulcus
of
the
CA1
field
Stratum
granulosum
of
the
dentate
gyrus
CA1
field
of
the
cornus
ammonis
© LLTECH 2011 17
18. FF-‐OCT
images
dis>nguish
meningiomas
from
meningeal
haemangiopericytoma
Meningioma
psammoma
–
diagnosis
possible
directly
on
FF-‐OCT
image
Collagen
bundles
Whorls
CalcificaIon
80%
of
meningiomas
are
benign.
Collagen
balls
Histologically,
meningioma
cells
are
relaIvely
uniform,
with
a
tendency
to
encircle
one
another,
forming
whorls
and
psammoma
bodies
(laminated
calcific
concreIons).They
have
a
tendency
to
calcify
and
are
highly
vascularized.
Areas
of
fibrosis
may
be
present.
18
© LLTECH 2011
19. RAT
BRAIN
:
Different
zoom
levels
16x13mm2
4x4mm2
1x1mm2
Advanced
Imaging
in
Bio
© LLTECH 2011 19
20. Spinal
cord
:
normal
Issue
100 µm
Arrows: Fibers transverse cut
500 µm
100 µm
© LLTECH 2011 20
22. Normal
skin
morphology
a
Epidermis
c
b
Collagen
d
e
500
µm
Pilosebaceous
unit
Sweat
gland
Adipocytes
Dalimier and Salomon Dermatology 224, 84-92 (2012)
©
LLTech
2012
© LLTECH 2011 Courtesy
of
Hopitaux
Universitaires
de
Genève,
Genève,
Switzerland
22
23. Skin
verIcal
reconstrucIon
from
en-‐face
slicing
for
layers
assesment
and
measurement
KN:
KeraInocyte
nuclei
Papillary
melanin
caps
BV:
Blood
vessels
Stratum
corneum
KN
KN
KN
Stratum
spinosum
BV
BV
BV
Dermis
50
µm
VerIcal
reconstrucIon
of
skin
model
KeraInocyte
nuclei
Stratum
corneum
Stratum
spinosum
Dermis
50 µm
©
LLTech
2012
© LLTECH 2011 Courtesy
of
Hopitaux
Universitaires
de
Genève,
Genève,
Switzerland
23
24. 3D
reconstrucIon
from
en-‐face
slicing
Epidermis
and
dermis
separated
by
sodium
bromide
Epidermis
Dermis
800
µm
x
800
µm
800
µm
x
800
µm
Wrinkle
Stratum
corneum
Stratum
spinosum
Dermal
papilla
Collagen
Blood
vessel
50 µm 50 µm
Reconstructed
verIcal
slice
Reconstructed
verIcal
slice
© LLTech 2012 © LLTECH 2011 Courtesy of Hopitaux Universitaires de Genève, Genève, Switzerland
24
25. Adipocytes
200
µm
9um
depth
27um
depth
60um
depth
Reconstructed
depth
slice
50
µm
© LLTECH 2011 25
26. Skin
pathologies:
basal
cell
carcinoma
discriminaIon
at
structural
level
Dense
peritumoral
stroma
© LLTech 2012 © LLTECH 2011 Courtesy of Hopitaux Universitaires de Genève, Genève, Switzerland
26
27. Zoom
on
basal
cell
carcinoma:
discriminaIon
at
cellular
level
Peritumoral
stroma
High
cell
density
200
µm
100
µm
©
LLTech
2012
© LLTECH 2011 Courtesy
of
Hopitaux
Universitaires
de
Genève,
Genève,
Switzerland
27
28. Skin
in-‐vivo:
fingerprint
imaging
in
3D
Fingerprints
3D
reconstrucIon
FOV
800
µm
x
800
µm
Sweat
ducts
Corneocytes
LightCT
arm
support
add-‐
on
allows
in
vivo
imaging
100 µm on
arm,
hand,
fingers
En-‐face
image
of
fingerprints
© LLTECH 2011 28
29. Skin
in-‐vivo:
skin
layers
imaging
en
face
and
in
verIcal
reconstrucIon
Stratum
corneum
Stratum
spinosum
Dermis
Stratum
corneum
Stratum
spinosum
Dermis
100
µm
EvaluaIon
of
stratum
corneum
thickness:
15
µm
© LLTECH 2011 29
30. Mouse
skin
in
vivo
20um
depth
En-‐face
slices
in
thigh
region
10um
depth
FOV
1mm2
PenetraIon
to
200um
Hairs
near
surface
35um
depth
85um
depth
Hair
follicles
Collagen
fibers
©
LLTech
2012
© LLTECH 2011 Courtesy
of
InsItut
Langevin,
Paris,
France
30
32. Cross
secIon
of
rat
lung
Scale
bar
=J
0.5
mm
nform
2011,
2:28.
Jain
et
al.
Pathol
I
© LLTECH 2011 32
33. Image
from
non-‐neoplasIc
human
lung
Issue
Collapsed
lung
Alveoli
Blood
vessel
FOV:
FFOCT
~
4
mm
x
4
mm;
H&E
=
4X
Jain
et
al.
Pathology
Visions
2013.
© LLTECH 2011 33
34. Adenocarcinoma
of
lung
with
lepidic
&
invasive
components
Invasive
Invasive
Lepidic
Lepidic
Invasive
Invasive
© LLTECH 2011 FOCT
~
4
mm
x
4
mm;
H&E
=
4X
.
Insets:
FFOCT
~
0.375mm
x
0.375mm;
H&E
=
20X
FOV:
F 34
36. Glomerulus
Image
from
non-‐neoplasIc
kidney
Issue
Glomerulus
Blood
vessel
3xzoom
(FOV
~0.19x0.19
mm)
Tubules
Tubules
3x
zoom
(FOV
~0.19x0.19
mm)
Blood
vessel
FFOCT:
FOV~
0.37x0.25
mm,
depth
of
imaging
0.1um
Caron
et
al.
Proc
SPIE
2013
© LLTECH 2011 3x
zoom
(FOV
~0.19x0.19
mm)
36
37. H&E
image
from
non-‐neoplasIc
kidney
Glomerulus
Blood
vessel
Glomerulus
20X
Tubules
Tubules
20X
H&E
=
10X
Caron
et
al.
Proc
SPIE
2013
© LLTECH 2011 Blood
vessel
20X
37
38. NeoplasIc
kidney:
normal
architecture
replaced
by
sheets
of
cells
Sheets
of
cells
Sheets
of
cells
Sheets
of
cells
FFOCT:
FOV
~
H&E
10X
0.38x0.38
mm,
depth
of
imaging
:
7.2um
3x
zoom
(FOV~
0.19x0.19mm)
© LLTECH 2011 Chromophobe
RCC
20X
38
40. ReIna
Photoreceptors - pig
Nerve
fiber
layer
-‐
rat
OpIc
Nerve
-‐
pig
Retinal pigment epithelium - pig
ReIna
-‐
rat
Grieve et al IOVS 2004 Nov;45(11):4126-31.
©
LLTech
2012
© LLTECH 2011 Courtesy
of
ESPCI,
Paris,
France
40
41. Human
graa
cornea:
over
500um
thickness
50
um
Ghouali
et
al,
ARVO
2013
www.lltechimaging.com
© LLTECH 2011 Courtesy of Vincent Borderie
41
42. Large
field
en
face
view
of
epithelial
layer
100
um
LightCT
can
evaluate
integrity
of
epithelium
and
stroma
in
graa
corneas
(not
possible
with
exisIng
techniques)
200
um
www.lltechimaging.com
© LLTECH 2011 Courtesy of Vincent Borderie
42
43. Large
field
en
face
view
in
stroma
200
um
ApplcaIon
of
LightCT
in
eye
banks
www.lltechimaging.com
© LLTECH 2011 Courtesy of Vincent Borderie
43
44. LightCT
shows
corneal
pathology
and
can
penetrate
even
edematous
cornea
Cornea
with
edema
Cornea
with
chemical
burn
–
highly
sca]ering
upper
stroma
© LLTECH 2011 44
46. Core
Needle
Biopsy:
Kidney
LightCT
provides
an
evaluaIon
of
Issue
architecture
in
minutes
100µm
Renal
tubules
500µm
50µm
Vessel
©
LLTech
2012
© LLTECH 2011 Courtesy
of
InsItut
Curie,
Paris,
France
46
47. Core
Needle
Biopsy
Breast:
InfiltraIve
carcinoma
LightCT
used
intraoperaIvely
on
biopsy
Issue
provides
fast,
non
destrucIve
evaluaIon
©
LLTech
2012
© LLTECH 2011 Courtesy
of
InsItut
Curie,
Paris,
France
47
50. In
vivo
imaging
of
drosophila
melanogaster
Four
stages
of
pupaIon
over
72
hours:
automated
Ime
series
Prepupa
(0-‐2
h)
TransiIon
to
pupal
stage
(24
h)
Pupal
stage
(48
h)
Advanced
pupal
phase
before
eclosion
(72
h)
© LLTech 2012 © LLTECH 2011 Courtesy of ESPCI, Paris, France
50
51. Pupa
118:
4
days,
30um
depth
100
um
LightCT
can
capture
automated
Ime
series
to
follow
in
vivo
development
over
a
period
of
days
Burcheri
et
al,
Proc
SPIE
2013
© LLTECH 2011 51
54. Xenopus
Laevis
eye:
en
face
slices
Musée d’Histoire Naturelle
Surface
10um
depth
20um
depth
30um
depth
40um
depth
50um
depth
60um
depth
70um
depth
80um
depth
140um
depth
230um
depth
180um
depth
200
um
© LLTECH 2011 54
55. C.
elegans
–
Images
at
higher
magnificaIon:
40X
100 µm
3D
reconstrucIon
50 µm
© LLTech 2012 © LLTECH 2011 Courtesy of ENS, Paris, France
55
57. Leaf
veins
–
fresh
leaf
sample
Veins
500 µm
Fibers
100 µm
57
© LLTech 2012 © LLTECH 2011 Courtesy of ESPCI, Paris, France
57
58. Apple
Wax
Skin
Water
Flesh
500 µm 100 µm
58
© LLTech 2012 © LLTECH 2011 Courtesy of ESPCI, Paris, France
58
59. Sorghum:
cross
secIon
Courtesy of Plate-forme
d'Histocytologie et d'Imagerie
Wide
field
en
face
Cellulaire Végétale (PHIV)
Montpellier RIO2011
© LLTECH Imaging
14mmX15mm
59
60. Sorghum
cross
secIon:
naIve
field
view
800um
x
800um
en
face
field
Reconstructed
depth
slices
500um
sample
thickness
Courtesy of Plate-forme
d'Histocytologie et d'Imagerie
Cellulaire Végétale (PHIV)
Montpellier RIO Imaging
© LLTECH 2011 60
61. Courtesy of Plate-forme
d'Histocytologie et d'Imagerie
Cellulaire Végétale (PHIV)
Rice:
hypocotyle
Montpellier RIO Imaging
Wide
field
en
face
5mmX2mm
Reconstructed
depth
slice,
200um
pentraIon
depth
© LLTECH 2011 61
62. Conclusion
• Full-‐field
OCT
does
not
require
Issue
preparaIon,
nor
staining
of
any
kind
• Creates
images
within
minutes
using
a
non-‐destrucIve
process
• Offers
a
1
µm
cellular
resoluIon
in
3D
• Reveals
structural
and
cellular
informaIon
of
normal
and
pathological
Issue
• Mosaicing
allows
fast
visualisaIon
at
various
scales
• En-‐face
high-‐resoluIon
imaging
allows
verIcal
and
3D
reconstrucIon
• Possible
applicaIons
for
biobanking,
cancer
detecIon,
digital
pathology…
www.lltechimaging.com
© LLTECH 2011 62
63. LLTech
LLTech,
Inc.
LLTech
103
Carnegie
Center
Drive
Pépinière
Paris
Santé
Cochin
Suite
300
29
rue
du
Faubourg
Saint
Jacques
Princeton,
NJ
08540,
USA
75014
Paris
Phone:
+1
609
995
3506
Phone:
+33
1
82
72
61
25
www.lltechimaging.com
contact@lltech.fr
www.lltechimaging.com
© LLTECH 2011 63