<ul><li>3 rd  meeting international research - June 10, 2011 </li></ul><ul><li>Infectious disease and Microbiology </li></...
Objective <ul><li>Completing the Genes repertoire </li></ul><ul><li>Microorganisms </li></ul><ul><li>Genomes </li></ul><ul...
Unknown genosphere by metagenomics studies <ul><li>10-15% organisms with Ribosomes </li></ul><ul><li>15-30% known viruses ...
U R <ul><li>TABASCO, Hot Ketchup and human stool </li></ul><ul><li>Positive detection by RT-PCR </li></ul><ul><ul><li>TEM ...
Tools    Culture- introducing culturomics
 
 
Free-living bacterial community Merhej V, et al.  Biol Direct . 2009 Apr 10;4:13. 264 lost COGs Facultative host-associate...
Genome sequencing and analysis
In point of care for diagnostic and treatment  POC  Timone  Senegal Molecular diagnostic
Results <ul><li>Giant viruses </li></ul><ul><li>Microbiota and obesity </li></ul><ul><li>Repertoire of causes fever in Afr...
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2.6 didier raoult

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2.6 didier raoult

  1. 1. <ul><li>3 rd meeting international research - June 10, 2011 </li></ul><ul><li>Infectious disease and Microbiology </li></ul><ul><li>Ignorance, speculative deductions, unconfirmed predictions </li></ul><ul><li>- H factor </li></ul><ul><li>- Highest grant = 73 M€- I.H.U </li></ul>Didier Raoult - [email_address] - Marseille - France U R
  2. 2. Objective <ul><li>Completing the Genes repertoire </li></ul><ul><li>Microorganisms </li></ul><ul><li>Genomes </li></ul><ul><li>Where </li></ul><ul><li>In environnement (water, goil, animals, vectors plants) </li></ul><ul><li>Healthy humans </li></ul><ul><li>Patients </li></ul><ul><li>Comparing repertoires to identify links with diseases </li></ul><ul><li>Microbes </li></ul><ul><li>Genomes </li></ul><ul><li>Genes </li></ul><ul><li>Tools for diagnostic </li></ul>
  3. 3. Unknown genosphere by metagenomics studies <ul><li>10-15% organisms with Ribosomes </li></ul><ul><li>15-30% known viruses </li></ul><ul><li>55-70% ??? </li></ul>DNA sequences of the living in the XXI th century Edwards RA, Rohwer F. Viral metagenomics. Nature Rev Microbiol. 2005;3:504-510. U R
  4. 4. U R <ul><li>TABASCO, Hot Ketchup and human stool </li></ul><ul><li>Positive detection by RT-PCR </li></ul><ul><ul><li>TEM ~ 10 9/ ml </li></ul></ul><ul><li>Is it multiplying in humans? </li></ul><ul><li>Is it causing something? </li></ul>P.Colson. Pepper Mild Mottle Virus, a Plant Virus Associated with Specific Immune Responses, Fever, Abdominal Pains, and Pruritus in Humans .Plos one.2010
  5. 5. Tools  Culture- introducing culturomics
  6. 8. Free-living bacterial community Merhej V, et al. Biol Direct . 2009 Apr 10;4:13. 264 lost COGs Facultative host-associated Bacteria GS 2.92 ± 1.6 GC 43.0 ± 11.7 Op 3.36 ± 2.6 TR 65.7 ± 56.0 615 lost COGs Core of lost genes Metabolism Cellular processes ↑ Atypical rDNA operon ↓ Growth rate
  7. 9. Genome sequencing and analysis
  8. 10. In point of care for diagnostic and treatment POC Timone Senegal Molecular diagnostic
  9. 11. Results <ul><li>Giant viruses </li></ul><ul><li>Microbiota and obesity </li></ul><ul><li>Repertoire of causes fever in Africa (45%) </li></ul><ul><li>New culture media (TB, T.whipplei , Coxiella burnetii for vaccine) </li></ul><ul><li>5) Reversion of pathogenocity paradigm more pathogenic less genes (except toxins) => vaccine interest </li></ul><ul><li>6) New antibiotics (pentamidine) </li></ul><ul><li>7) Multiplexed serologic microaray </li></ul>
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