1. Sagarika Biswas; Saurabh Sharma;Ashish Saroha;D. S.
Bhakuni; Rajesh Malhotra; Muzna Zahur;Michael Oellerich; Hasi
R. Das; Abdul R. Asif.
-Juan Esteban Quiroz Álvarez
-Jose Alejandro Ramirez Quiros.
III semester.
Faculty of medicine.
UPB
March 11/ 2013

3. • Chronic inflammatory disease.
• Synovitis.
• Joint destruction
peripheral destruction
pain, stiffness, swelling and loss
of mobility.
-Changes in immune system.

4. • Cell Membrane Proteins.
• Function: cell recognition.
• Genes located on
chromosome 6.
• They (immune cells) attack
foreign antigens or
anomalous.

5. Viscous fluid (Hyaluronic
acid and low protein)
Nutrients to the Reduces friction
articular cartilage between bones
Lubrication to the joints

6. • Search for proteins by antibody binding to these specific proteins.

7. Relationship
Synovial Fluid Autoantigen Inmunoproteomic

11. • 131 personas.
• Entre 25-65 años.
• De ellos, 60 se tomaron muestras de
los pacientes con AR (35 mujeres,
25 hombres), 30
• de osteoartritis (OA) (20 mujeres, 10
varones), 01
• trauma de paciente, y 40 muestras
sanas (20 mujeres, 20 varones) se
recogieron como control negativo.

12. • 1D (Enfoque isoeléctrico) Separación y análisis
1 de proteinas.
• 2D (SDS)
2
• Sistema Mini Protean II

13. Proteinas se transfieren a un filtro
Luego de la electroforesis
Con Anticuerpos
Detectar proteinas
especificas en una muestra
determinada. Estos reaccionan con la
proteina de interes

14. 1.Puntos de proteinas llevados a cubos de geles de 1mm.
2. Se lavo con agua destilada.
3.Se destiño
4. Se deshidrato
ACN (Acetonitrilo) permitió la extracción de péptidos, se seco y
se almaceno en 0.1% de acido fórmico para su posterior
análisis.

15. Incubaron
Muestras en Electrotransferencia de con los 5
SDS proteinas a la membrana anticuerpos
primarios
Resultados: Con un valor
significativo.
Presencia de autoanticuerpos
contra GFAP y A1BG.

16. Cuantificar la cantidad de
autoanticuerpos en los pacientes
con AR.
En este caso es un ensayo indirecto :
-Luego del lavado…. Se le añade Ig
antiglobulina humana . La cantidad del
producto es directamente proporcional a la
cantidad de Ac presente en la muestra.

26. • I really enjoyed the seminar because it had a very clinical
approach, in turn feel that certain paragraphs were really
tangled, but overall I loved it.
• Looking to the future is very productive these autoantigens
discovery because it is a disease that occurs much worldwide.
• With these search of autoantigens science progresses every
day, thanks to early detection of certain diseases and their
best treatment.
• The characterization of these proteins in rheumatoid arthritis
will be very useful for understanding the pathogenesis of the
disease.

27. • The immunoassay, like western blot, have a bigger
importance to know the etiology of varietys disease.
• If we recognize the most common autoantigens in AR,
we could prevent early the advance of the patology.
• Western blot have a great especificity in the
identification of which antigen rected more whit an
antibody the plasm.
• Is necesary found others markers of RA, different to the
Rheumatoid factor, because it dont express en 1/3 of
patients.

30. • Martinez Sanchez, Lina Maria; Molecular biology;
7ed, Medellín: UPB, Faculty of medicina; 2012.