Knysna and Monotropa uniflora by Cole Steber

Travel Technology

Summer 2011 Cole Steber Knysnaand Monotropauniflora

Summer Research Project with monotropauniflora Microsatellite Primer Development Sampling trip Back in Danville…

Microsatellite(n): sequence composed of tandem repeat units usually two to five nucleotides in length (TC)12 CTAGGAGAGGTATATATGGGTCTTAAAATGTTTTAGATCATTATTTCAGATCTAAGTTCTCTCTCTCTCTCTCTCTCTCTCTCCACTTGAAGAACTCTAGACCTCTCTGTCCCTTCTCTTTTGATTTTGAGGTGGTTCCA

Microsatellite as a Tool Fine scale population analyses Highly variable DNA Polymerase stutters Repeat units added or deleted DNA repair mechanisms not 100% effective http://studentreader.com/files/repeat_microsatellite.png

Monotropauniflora(Indian Pipe) Uniflora has large variation in color and in blooming periods Variation is evident across sympatric and allopatric populations

Characteristics Heterotrophic Grows 10-30 cm Predominantly underground Mycorrhizal Associates Russula Lactarius

Plant and Fungus Relationship 90% of plants have some association with mycorrhizal fungi Mutualism

Explanation for Variation Speciation Temporal reproductive isolation Cryptic speciation through mycorhizal fungal associate interactions Null hypothesis: No genetic differentiation among blooming periods

Final Progress Future Research: Genotyping the populations of extracted DNA Study associations of mycorrhizal associates

Genetic Fingerprint Combine 10 different loci to get one fingerprint per individual.

Exploration of the Medical Field Assisted career decision making Exploration of Scientific Research Learned how scientific process works Gained an appreciation for conservation and nature Summer Enrichment

To the James Grahm Brown Foundation and all my mentors who assisted and made my summer experience possible A Sincere Thank You

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Summer was broken down into two parts. The first half consisted of participating in the University of Louisville's Student Orientation Staff (SOS’er). As an SOS’er it was my responsibility to introduce the incoming Freshmen to the University, specifically I worked with the Speed School of Engineering students and helped to build many of the students' first semester schedules. The second part of the summer started my internship with Joe Dablow in the Enrollment Management office. In this internship I have applied the principles of Industrial Engineering to Enrollment Management to forecast retention rates of student cohorts and analyze the makeup of each class (original freshman, transfer, etc.). This internship will continue through the Fall semester.

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This summer provided an opportunity to explore my Middle Eastern Studies degree in a professional context. I spent ten weeks interning in the Arabic Language and Culture Initiative of the Washington D.C. based Qatar Foundation International. My responsibilities included compiling and coding the first database on the needs/concerns/status of Arabic programs in American schools. However, my capstone project was coordinating and executing a documentary of Arabic programs in American K-12 schools. Two weeks of filming took coworkers and me to Seattle, Portland, Oakland, Los Angeles and Chicago to interview parents, students and teachers in the Arabic instruction community. Seeing Arabic instruction’s power to build cultural empathy in communities across the U.S. strengthened my passion for Arabic acquisition. I leave this summer with a continuation of my internship, as I have been hired to help edit the documentary, and a direction for grad school and life after Centre.

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I conducted a long-term research project comparing the French and American legal systems, focusing on three major components: the type of law practiced, the role of a judge in each legal system, and the perception of lawyers by others in each culture. . This project combined traditional library research with a series of personal interviews conducted in Strasbourg, France. Through my research I discovered the organizational and conceptual differences between each country as well as the creation and fulfillment of law within society while the personal interviews allowed me to discuss the perception of lawyers within society. I plan to continue conducting parallel interviews and completing more research throughout the fall semester and present my findings at Centre’s annual RICE symposium. This project was one that combined my interests in French language and culture with my plans to pursue the study of law after graduation.

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Blood Diseases bananas in North Minahasa Regency, North Sulawesi, Indonesia caused by Ralstonia solanacearum phylotype IV has existed for decades, but over the past few decades there has been no data on insect visitors banana flower which acts as a carrier of this pathogen. This study aims to determine the insects visitors banana flower can be a vector of R. solanacearum phylotype IV and the number of propagules of these bacteria carried by these insects. In every garden selected by purposive sampling of five to seven flowering tree, then flower-visiting insects were captured using a modified insect net. Insects were sorted according to species and counted the number of each species. Five individuals of each species of insects were placed in pots. Isolation of Ralstonia solanacearum phylotype IV using spread-plate method, then colony forming units (CFU) of virulent R. solanacearum filotipe IV per milliliter was calculated. Oscinella sp was an insect species most committed feeding on banana cv. kepok inflorescence (42.39 individuals), followed by A. melifera (1.47 individuals), Formicidae (0.65 individual), Blaberidae (0.55 individual), and Chelisoches morio (0.39 individual). Oscinella sp. brought 17636.39 to 75533.33 CFU / ml R. solanacearum phylotype IV, Aphis melifera 23500.00 to 26100.00 CFU / ml, Blaberidae 10300.00 to 23690.00 CFU / ml, Formicidae 3600.00 to 8000.00 CFU / ml, and C. morio 340.00 to 8000.00 CFU / ml.

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