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Frolicking in France by Brittany Hubert


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Frolicking in France by Brittany Hubert

  1. 1. Frolicking in FranceSummer Enrichment Trip 2011<br />By Brittany Hubert<br />
  2. 2. Getting Started<br />Interested in Molecular Biology<br />Medical School?<br />Not Really..<br />Research?<br />I think so…<br />Started Lab Work with Dr. Perlin<br />Growing Interest in Research<br />
  3. 3. My Project<br />Biology Research from different aspects<br />University lab, service work, research facility, other..??<br />Different Areas of Molecular Biology<br />Virology, Immunology, Bacteria, Fungus<br />Find what fits me<br />Gain experience in all areas<br />Learn to think like a “scientist”<br />Use experience to advance in my personal education experience<br />Form lasting relationships with experienced professionals<br />
  4. 4. Summer 2011<br />Seven weeks at University of South Paris<br />Orsay, France<br />About 13 miles outside of Paris<br />Work with Tatiana Giraud PhD and lab team <br />The fungus, Microbotryum<br />3 Goals of Project<br />Learn as much as possible about plant infections, microsatellites, and inoculations<br />Gain general lab experience<br />Immerse myself in French culture and practice language for possible minor<br />
  5. 5. Gettin’ Down to Business<br />Fungus Microbotryum: <br />Anther Smut Disease<br />Infects anthers of flowers in carnation family (Silenelatifolia & Silenedioica)<br />Topics<br />Glacial refugia using microsatellites- repeated units of DNA characteristic of different strains<br />Hybrid Sterility/Gene Flow between and within clusters<br />Competetive Exclusion<br />My Jobs<br />PCR with Odile and Pierre (microsatellites)<br />Making and recording conjugations<br />Garden work with Laetitia<br />Replating and recording good strains<br />Healthy<br />Infected<br />“Smutted”<br />
  6. 6. My Lab Team!<br />Odile<br />Pierre<br />Mandy<br />Tatiana<br />Alex<br />Marina<br />Elsa<br />
  7. 7. Microbotrym in a Nutshell..or Meristem<br />The Lab<br /><ul><li>Lab techniques
  8. 8. PCR, Pipetting, Conjugations
  9. 9. Working with a Team
  10. 10. Reading (and understanding) research articles
  11. 11. Making Charts
  12. 12. Reasoning</li></ul>Microbotryum<br />Easily cultured in lab<br />Reproduces quickly<br />Grows easily on petri dishes<br />Spread by pollinators in nature<br />(bees, bugs..etc)<br />Infects only meristems-embryonic tissue in plants; undifferentiated, growing, actively dividing cells. <br />(<br />Host specific<br />Followed same speciation path as hosts after glaciations<br />High selfing rate/low crossing rates<br />Closely related species can reproduce<br />Genetic distance determines reproductive ability<br />
  13. 13. Why it Matters!<br />Allows us to:<br />Understand other past disease patterns<br />Predict future patterns<br />Understand interactions between host-specific or parasitic organisms<br />Start work on disease prevention or cures<br />Glacial Refugia<br />Silenelatifolia<br />Smut Life Cycle<br />Silenedioica<br />
  14. 14. Biology on the Beach<br />Four Day Biology Conference<br />In Roscoff (Brittany), France<br />On New and Emerging Fungal Diseases of Animals and Plants in the context of evolution<br />Interesting talks on many different areas<br />Eye-opening<br />Confusing<br />Learned <br />What conferences are all about<br />New material<br />What I don’t know..<br />Met many important people/Made new acquaintances <br />Spent a day on the beach!<br />
  15. 15. Things you learn when you travel completely alone!<br />Goals<br /><ul><li>Gain more self-confidence-check!
  16. 16. Become more independent-check!
  17. 17. Expand my comfort zone-check!</li></ul>How (and who) to ask for help<br />How to use multiple maps<br />What it takes to entertain myself<br />How interesting other people’s lives can be<br />The Paris metro system (after a while)<br />Exactly what you like and don’t like<br />How to adjust to different cultures <br />Travel to France, Switzerland, Germany, and Rome<br />
  18. 18. Goals for the Future<br />Continue work in Dr. Perlin’s lab<br />Explore different areas of Molecular Biology research<br />Not lab work (monotonous)<br />More dynamic area<br />Possible work with Pasteur Institute or Marie Curie institute in Africa<br />Look into forensic fields<br />
  19. 19. Special thanks to the James Graham Brown Foundation!!<br />And thanks to:<br />-Andrew Grubb <br />-Dr. Michael Perlin<br />Pictures with no citation are from or my camera!<br />