Cell Disruption is the method or process for disrupting or lysing the cell inorder to release the contents out of the cell. Purpose Cell disruption is done to release the cell contents. Cell Lysis or Disruption is done for isolating Nucleic acids (DNA / Plasmids), Proteins (Intracellular Proteins), Organelles, etc.

1. Cell Disruption – Methods,
Advantages & Disadvantages
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2. Cel l Di srupt i on / Cel l Lysi s:
 Cell Disruption is the method or process for disrupting or lysing the
cell inorder to release the contents out of the cell.
 Purpose
 Cell disruption is done to release the cell contents. Cell Lysis or
Disruption is done for isolationg Nucleic acids (DNA / Plasmids),
Proteins (Intracellular Proteins), Organelles, etc.

3. Met hods
Physical methods
Chemical Methode
Enzymatic Methods

4. Cel l -di srupt i on / Cel l
Lysi s
Physical Methods
Manual Grinding:
Sonication:
Liquid Homogenization:
Freeze Thaw Method:

5. Chemi cal Met hods
Most widely used Detergents are:
Triton X – 100
Tween 20
Tween 80
Sodium Dodecyl Sulfate (SDS)
CHAPS
Enzymatic methods are easy and fast but cost for these
methods are also high. Lysozymes are used for
Bacterial cell lysis and Chitinase for Yeast cell
lysis.Pectinases are used for the lysis of plant cell walls
for isolating protoplast and other applications.

6. Appl i cat i ons of Cel l -di srupt i on
 Isolating intracellular Proteins.
 Downstream Processing.
 Isolating intracellular organelles.
 Nucleic acid isolation.

7. Di sadvant ages
 whole cell contents are released out which makes it difficult to
separate out product of interest from the mixture.
 Cell lysis increases viscosity of the solution making it difficult to
process in the further steps.
 Product released into harsh environment causing the product to
lose stability or activity.
 Might cause filtration membranes to foul during the filtration
process.
 Enzymatic cell-disruption in large scale can be expensive.

8. Commerci al l y Avai l abl e Cel l Di srupt ors
/ Cel l Lysi s Buf f ers / Enzymes
 Qsonica Ultrasonicator
 CelLyticTM MT - Mammalian Tissue Lysis/Extraction
Reagent - Sigma Aldrich
 Glo Lysis Buffer (GLB) - Promega

9. Ref erences

Tutorial on Cell Disruption - TELIOS
T. TZANNIS - RPI 1991
Thermo Scientific Technical Resource

10. Thank You
Click here to visit Bio-
resource for more detailed
information on Cell
Disruption, Types,
Applications, Advantages
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