Mature miRNAs are naturally occurring, 22-nucleotide, non-coding RNAs that mediate post-transcriptional gene regulation. Mature miRNAs possess a 3’ hydroxyl group and a 5’ phosphate group (unlike most other cellular RNAs). Due to this, adapters can be ligated to both the 3’ end and 5’ end of mature miRNAs, enabling universal reverse-transcription and pre-amplification, while minimizing the background from other RNA species. From cell lysis through preamplification, the miScript Single Cell qPCR Kit is a robust, universal solution for precision single-cell miRNA expression. An entire miRNome expression profile can be generated from an individual cell – explore the power of one.

1. Sample to Insight
miRNA analysis from single cell
Learn about how you can quantify all (annotated and non-annotated) miRNA from single cell

2. Sample to Insight
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Discover the miScript Single Cell qPCR Kit
• Generate miRNome expression profiles from a single cell
• Integrated reaction controls for each step of the workflow
• Real-time PCR analysis with the largest portfolio of bench-verified miRNA assays
.
Single cell sample
input
Universal miRNA
cDNA synthesis &
preamplification
Tailored data
analysis
*MDA = multiple displacement amplification
Single cell genomics by QIAGEN, 2016

3. Sample to Insight
miScript Single Cell qPCR Kit
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For miRNA expression profiling from a single cell
Primary
sample
isolation
Single cell
isolation
Cell lysis,
RT &
miRNA
preamp
Sample
• One to 100 cells
• 25 pg to 1 ng of purified RNA
• qPCR analysis with miScript miRNA PCR Arrays
• Data analysis with GeneGlobe Data Analysis Center
InsightPCR
Data
analysis
Interpretation
Single cell genomics by QIAGEN, 2016

4. Sample to Insight
miScript Single Cell qPCR Kit
4
PCA reveals a heterogeneous response amongst individual cells to 5-aza-2’-deoxycytidine treatment. HCT 116
colorectal cancer cells were treated ± 5-aza-2’-deoxycytidine (5-aza-2’-dC) demethylation reagent. Bulk cell pellets (Panel
A) and individually isolated treated cells (Panel B) were processed using the miScript Single Cell qPCR Kit, and the
miFinder miScript miRNA PCR Array was used in real-time PCR. For the bulk sample analysis (Panel A), a scatter plot of
2-ΔCT values demonstrates that differential miRNA expression was observed in response to the 5-aza-2’-dC treatment.
PCA analysis of individual cells (Panel B) reveals a differential response amongst the treated cells with three distinct
populations. Together, the data suggests the consequential “cellular averages” of bulk analyses mask intrinsic
transcriptional variability across individual cells.
Single cell genomics by QIAGEN, 2016

5. Sample to Insight
miScript Single Cell qPCR Kit
5
The miScript Single Cell qPCR Kit enables sensitive detection of low abundance miRNAs. (A) 10-fold dilutions of HeLa
S3 purified RNA (1 ng, 100 pg and 10 pg) were processed using the miScript Single Cell qPCR Kit, and the human
miFinder miScript miRNA PCR Array was used in real-time PCR. (B) 10-fold dilutions (1x104 copies to 10 copies) of a
pool of 24 synthetic miRNAs were processed using the miScript Single Cell qPCR Kit, and miScript Primer Assays
were used in real-time PCR. With increasing 10-fold dilutions of RNA or synthetic miRNAs, the CT unit decreased
accordingly demonstrating the sensitive detection capacity of the miScript Single Cell qPCR Kit
Single cell genomics by QIAGEN, 2016
Read the white paper Extending miRQC‘s dyamic range: amplifying the view of limiting RNA samples

6. Sample to Insight
Single cell genomics by QIAGEN
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For in-depth, molecular analysis of single cells
Single cell
WGA or WTA
Single cell
isolation
Single cell
sequencing
• Easily access
single cells
• Affordable
• Gentle on cells
QIAscout
QIAseq
FX Single Cell
Library Kits
REPLI-g
Single Cell Kits
Single cell
miRNA analysis
• Create superior-
quality NGS
libraries directly
from single cells
• Provides best-in-
class amplification
of genomes or
transcriptomes from
single cells
• Profile miRNA
expression using
qPCR
miScript
Single Cell
qPCR Kit
Single cell genomics by QIAGEN, 2016
Visit the single cell resource center