Virus Super Resolution Microscopy: A further example for the use of localization microscopy/SPDM is an analysis of Tobacco mosaic virus (TMV) particles. TMV particles are not only important in plant research but are also an example for a class of most promising biomolecule complexes exhibiting a high potential for nanotechnology applications as virus-derived biotemplates for nanostructured hybrid materials and building-blocks in analytical, technical & therapeutic applications. Collaboration of the Cremer & the Wege labs (Universities of Heidelberg & Stuttgart). Reference: Superresolution imaging of biological nanostructures by spectral precision distance microscopy (2011): Cremer, R. Kaufmann, M. Gunkel, S. Pres, Y. Weiland, P- Müller, T. Ruckelshausen, P. Lemmer, F. Geiger, S. Degenhard, C. Wege, N- A. W. Lemmermann, R. Holtappels, H. Strickfaden, M. Hausmann , Biotechnology 6: 1037–1051

1. Virus super resolution microscopy
Localisation microscopy: SPDMphymod images of Tobacco mosaic virus (TMV) particles with
coated proteins labeled with the fluorophore Atto448.
B: after Gaussian blurring corresponding to the individual localization accuracy
D: same data, but distribution of the individual molecule position without Gaussain blurring
Collaboration of the Cremer & the Wege labs (Universities of Heidelberg & Stuttart). Superresolution imaging of biological nanostructures
by spectral precision distance microscopy (2011): Cremer, R. Kaufmann, M. Gunkel, S. Pres, Y. Weiland, P- Müller, T. Ruckelshausen, P.
Lemmer, F. Geiger, S. Degenhard, C. Wege, N- A. W. Lemmermann, R. Holtappels, H. Strickfaden, M. Hausmann , Biotechnology 6: 1037–