PRP Platelet Rich Plasma is a non-surgical, non-invasive treatment for hair loss. “Cell therapy” is a relatively new frontier in medical technology which involves introducing cells into a tissue in order to treat a disease or condition. Areas of research such as stem cell processing and activation, cell multiplication, cytokines, growth factors, extracellular matrix products, bioscaffolds, platelet rich plasma (PRP), gene therapy, tissue repair and more are some of the exciting new ways in which scientists are investigating in the use of cell therapy to physicians improve outcomes and treat a variety of conditions.
Adjunctive therapies which impact wound healing like the use of hyperbaric oxygen, copper peptide solutions, phototherapy, extracellular matrix (ECM), protein soaks, etc. have been a part of Dr. Alan Bauman’s post-operative treatment regimens after hair transplantation for years. Helping the body safely “help itself” is the cornerstone of these types of treatments which often utilize natural components found in the human body. Along those lines, PRP has already been found to be a helpful adjunct successfully in use in many areas of medicine, including orthopedics, dentistry and plastic surgery for tissue repair, tissue regeneration and wound healing.
What is Platelet Rich Plasma (PRP)?
Produced in just minutes from a small quantity of your own blood, PRP contains a wide variety of the body’s own natural growth factors which may be helpful in accelerating tissue regeneration, improving healing time and stimulating hair growth. Platelets are cell fragments that are normally found in the blood and play a key role in blood clotting and are a natural source of growth factors. Special medical equipment is used by your physician to separate a platelets from a small sample of your own blood and prepare them for use. Once applied and activated, platelets release their stores of powerful growth factors and other molecules.
What Growth Factors are found in Platelet Rich Plasma (PRP)? The platelets collected in PRP are activated by thrombin and calcium chloride to induce the release of these growth factor proteins and other cytokines:
• platelet-derived growth factor
• transforming growth factor beta
• fibroblast growth factor
• insulin-like growth factor 1
• insulin-like growth factor 2
• vascular endothelial growth factor
• epidermal growth factor
• interleukin-8
• keratinocyte growth factor
• connective tissue growth factor
HOW IS PRP MADE?
PRP is made from a small sample of your own blood. The FDA-approved process concentrates the platelets found in the blood sample and separates them from other blood components. These concentrated platelets contain huge reservoirs of growth factors and wound healing factors. Growth factors are natural components of your body whose potential benefits can now be harnessed using modern medical technology. For hands-on PRP training classes v
1. Alan J. Bauman, M.D.
Diplomate, American Board of Hair Restoration Surgery
YES PRP Certification & Training
May 30th, 2015
PLATELET RICH PLASMA
FOR HAIR REGROWTH
ABHRS
2. CONFLICTS OF INTEREST
• The following conflicts of interest
pertain to this presentation: NONE
3. ALAN J. BAUMAN, M.D., A.B.H.R.S.
• Diplomate, American Board of Hair Restoration Surgery
• Member, International Society of Hair Restoration Surgery
• Recommended, American Hair Loss Association
• Member, American Academy of Anti-Aging Medicine (1998)
• New York Medical College
• Beth Israel Medical Center NY – General Surgery
• Mt. Sinai Medical Center NY – General Surgery
• Bauman Medical Group (est. 1997) - Boca Raton, FL
• >17,000 patients treated, >7,000 hair transplants
Alan J. Bauman, M.D.
4. VIDEO: PRP HAIR REGROWTH ON ABC
http://hai.rs/hairprp-placenta
5. OVERVIEW
• Hair Follicle & Growth Cycles
• Hair Loss & Treatments
• PRP & Growth Factors
• PRP in Hair Restoration
• Role of Extracellular Matrix (ECM)
• ACell/Matristem & BioD-Restore
• Hair Evaluations & Measurements
• How to Prepare & Apply Scalp PRP
• Additional Educational Opportunities
8. HAIR FOLLICLE
STEM CELL SIGNALING
• Epithelial Wnt/β-catenin signaling is required for hair
matrix cell proliferation. Wnt/β-catenin signaling is a
central regulator of adult stem cells.
• LRP inhibition permits stem cell survival; blocks hair
growth reversibly
• β-catenin is required in bulge stem cells for their
proliferation, but not survival indicating the
persistence of functional progenitors so follicles will
continue to proliferate even without β-catenin.
• Hair Follicle ~ Fibroblast “Cousin”
10. • Fear/anxiety ~ Sickness or Illness
• Low Self-Esteem/Self-Confidence
• Feel Older / Less Attractive
• Camouflage/Compensate
• Avoid Social Situations
HairLoss
11. HAIR LOSS: ANDROGENETIC ALOPECIA (AGA)
• Genetic Male and Female Pattern Hair Loss – most common type
• Affects nearly 100 Million Americans (est. 60M men, 40M women)
22. LITERATURE REVIEW:
USE OF PRP IN HAIR RESTORATION
• Graft-storage media in Hair Transplantation
(Bouhanna/Amgar)
• Wound healing accelerator in Donor and
Recipient Areas (R Reese, J Cole, J Cooley)
• Improvement of ‘take-rate’ & growth in hair
transplantation (R Reese, C Uebel)
• Stand alone treatment for androgenetic alopecia
or alopecia areata. (J Greco, Amgar, Bouhanna,
Rinaldi) Bouhanna/Amgar
28. EXTRACELLULAR MATRIX (ECM)
• Extracellular part of multicellular structure
• Structural & Biochemical support
• Injury Repair & Tissue Growth
• Wound healing / fibrosis
• Regulates intracellular communications
FUNCTIONS during injury or tissue regeneration:
• Prevents inflammation & scar tissue formation
• Facilitates tissue repair
29. WHY USE EXTRACELLULAR MATRIX (ECM) W/ PRP?
PROLONG & ENHANCE!
• Without ECM: PRP effects last ~90 days, requiring repeat treatments
• With ECMs
• PRP hair growth effects may last for up to 2 years
• PRP hair growth effects appear to be stronger
35. HAIR MEASUREMENTS & EVALUATIONS
• “HairCheck” – Cross-Section Hair
Bundle Trichometry
• HMI – Hair Mass Index
• Sensitive, scientific, repeatable,
accurate
• “HairCam” Dermoscopy – identifies
miniaturization
• Global Photos – medical ‘mugshots’
HairCam – ABC Good Morning America
HairCheck at Bauman Medical
36. HOW TO PREPARE AND APPLY PRP+ECM
• Patient Selection, treatment area identified & measured.
• Phlebotomy is performed.
• Scalp prepped w/ antiseptic shampoo and local anesthesia.
• PRP is prepared using centrifuge & YesPRP™ kit.
• If used, BioD-Restore is thawed.
• PRP is mixed gently with ECM (Acell/Matristem or BioD-Restore)
• PRP+ECM is injected intradermally into the scalp
• Mechanical (MicroPen) microneedling creates microtrauma
• Scalp is cleansed, 15min LLLT applied
• instructions given prior to discharge
Dr. Bauman performs PRP on ABC Palm Beach
37. SIDE EFFECTS / SEQUELAE / FOLLOW-UP
• Tenderness / Swelling is rare
• Follow-ups can be performed monthly to track hair growth improvements.
• Monitor hair growth using HairCheck and HairCam to observe peak and plateau.
38. UPCOMING
PRP HAIR GROWTH & HAIRCHECK™
WORKSHOPS
Friday June 19th -or- Friday Septembe 25th 9am-5pm Boca Raton
• Hair Loss Diagnosis & Evaluations, Treatments & Tracking
• Hands-on HairCheck Measurements
• Hands-on PRP+ECM hair regrowth procedure
• Course Director: Alan J. Bauman, M.D.
• Certificate, Starter Kit & Ongoing Support
• andres@baumanmedical.com or www.HairCoach.net for info/registration and future events
39. REFERENCES
Marx, R.E. , et al, “Platelet-Rich Plasma Growth Factor Enhancement for Bone Grafts,” Oral Surg Oral Med Oral Patrhol, 1998;85:638-646.
Antonaides, H.N., et al,”Human Platelet-Derived Growth Factor: Structure and Functions”, Federation Proceedings, 1983;42:2630-2634.
Pierce, G.F., et al,”PDGF-BB,TGF-β 1 and Basic FGF in Dermal Wound Healing: Neovessel and Matrix Formation and Cessation Repair”, Am J Pathology,
1992;140:1375-1388.
ELISA Immuno Assay For PDGF-A, Human VEGF, TGF-β 1, and Human EGF, R&D Systems, Mpls, MN 1999.
Bauman AJ, The use of cross-sectional hair bundle trichometry in the diagnosis, evaluation and tracking of hair loss. Poster Presentation. ISHRS ASM
October 2012 Nassau, Bahamas.
Gruss, J., et.al., Human amniotic membrane: a versatile wound dressing. CMA Journal 1978; Vol.118:1237-1246
De Coppi, P. et.al., Isolation of amniotic stem cell lines with potential for therapy. Nature Biotechnology 2007; Vol.25,No.1:1274-1280
41. Alan J. Bauman, M.D.
Bauman Medical Group
Boca Raton, FL USA
doctorb@baumanmedical.com
baumanmedical.com
linkedin/in/alanjbauman
twitter.com/DrAlanBauman
facebook.com/alanjbauman
Thank You!!