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PLATELET RICH PLASMA 
FOR HAIR REGROWTH 
Alan J. Bauman, M.D. 
Diplomate, American Board of Hair Restoration Surgery 
PRP ...
CONFLICTS OF INTEREST 
• The following conflicts of interest 
pertain to this presentation: NONE
ALAN J. BAUMAN, M.D., A.B.H.R.S. 
• Diplomate, American Board of Hair Restoration Surgery 
• Member, International Society...
VIDEO: PRP HAIR REGROWTH ON ABC 
http://hai.rs/hairprp-placenta
OVERVIEW 
• Hair Follicle & Growth Cycles 
• Hair Loss & Treatments 
• PRP & Growth Factors 
• PRP in Hair Restoration 
• ...
HAIR & SKIN ANATOMY: THE PILOSEBACOUS UNIT
HAIR GROWTH CYCLE
HAIR FOLLICLE 
STEM CELL SIGNALING 
• Epithelial Wnt/β-catenin signaling is required for 
hair matrix cell proliferation. ...
Hair Loss 
• Fear/anxiety ~ Sickness or Illness 
• Low Self-Esteem/Self-Confidence 
• Feel Older / Less Attractive 
• Camo...
HAIR LOSS: ANDROGENETIC ALOPECIA (AGA) 
• Genetic Male and Female Pattern Hair Loss – most common type 
• Affects nearly 1...
60X & 30X “HAIRCAM” DERMOSCOPY 
NORMAL MINIATURIZED
SEVERE DEPLETION: HAIR TRANSPLANTATION
HAIR LOSS: ALOPECIA AREATA (AA) 
• Autoimmune 
• Small / Circumscribed 
• “Spot baldness” 
• 1-2% of cases spreads 
to the...
MEDICAL HAIR LOSS TREATMENTS: 
PRESERVE AND ENHANCE HAIR FOLLICLE FUNCTION 
MODAL ITY TX MECHANISM 
Oral Finasteride block...
PLATELET RICH PLASMA 
FOR HAIR REGROWTH
GROWTH FACTORS, CYTOKINES AND MORE… 
• platelet-derived growth factor - PDGF 
• transforming growth factor beta - TGF-B 
•...
GROWTH FACTORS & HAIR FOLLICLE CYCLING 
• PDGF – stimulate growth of dermal mesenchyme, epidermis-follicle interaction (5)...
PLATELET RICH PLASMA STAND-ALONE 
TREATMENT FOR HAIR REGROWTH 
• Autologous (or Allograft) 
• Bedside 
• Out-patient 
• Qu...
VEGF 
VASCULAR ENDOTHELIAL GROWTH FACTOR 
Bouhanna/Amgar
LITERATURE REVIEW: 
USE OF PRP IN HAIR RESTORATION 
• Graft-storage media in Hair Transplantation 
(Bouhanna/Amgar) 
• Wou...
PRP FOR ANDROGENETIC ALOPECIA 
Bouhanna/Amgar
PRP FOR ALOPECIA AREATA 
PreTx 8 weeks 4 months
PRP FOR ALOPECIA AREATA 
PreTx 8 weeks 4 months
PRP HAIR REGROWTH IN 38YO FEMALE AGA 
BEFORE & AFTER 9MOS
PRP HAIR REGROWTH 36YO MALE AGA 
BEFORE & AFTER 9MOS
EXTRACELLULAR MATRIX (ECM) 
• Extracellular part of multicellular 
structure 
• Structural & Biochemical support 
• Injury...
WHY USE EXTRACELLULAR MATRIX (ECM) W/ PRP? 
PROLONG & ENHANCE! 
• Without ECM: PRP effects last ~90 days, requiring repeat...
ACELL/MATRISTEM ECM 
• Porcine Bladder Matrix 
• Micronized powder 
• Collagen 
• Basement membrane 
• Bio-scaffold
BIOD-RESTORE - PLACENTAL TISSUE ECM 
BioD-Restore™ is a cryopreserved amnion allograft from the placenta of live, healthy ...
BIOD-RESTORE 
• Multipotential Tissue Matrix 
• Structural allograft derived 
from human amnion 
• Chemical compounds, gro...
BIOD-RESTORE 
PROCUREMENT & PROCESSING: 
• Prescreened donors 
• Medical review 
• Prenatal testing 
• Behavioral risk ass...
GROWTH FACTORS FOUND IN AMNION 
• Platelet-Derived Growth Factor (PDGF) 
• Transforming Growth Factor Beta (TGF-β) 
• Vasc...
HAIR MEASUREMENTS & EVALUATIONS 
• “HairCheck” – Cross-Section Hair 
Bundle Trichometry 
• HMI – Hair Mass Index 
• Sensit...
HOW TO PREPARE AND APPLY PRP+ECM 
• Patient Selection, treatment area identified & measured. 
• Phlebotomy is performed. 
...
SIDE EFFECTS / SEQUELAE / FOLLOW-UP 
• Tenderness / Swelling is rare 
• Follow-ups can be performed monthly to track hair ...
UPCOMING 
PRP HAIR GROWTH & 
HAIRCHECK™ WORKSHOPS 
Friday September 19th -or- Friday November 14th 9am-5pm Boca Raton 
• H...
REFERENCES 
Marx, R.E. , et al, “Platelet-Rich Plasma Growth Factor Enhancement for Bone Grafts,” Oral Surg Oral Med Oral ...
PRP VIDEO PLAYLIST 
http://hai.rs/prphairgrowthvids
Alan J. Bauman, M.D. 
Bauman Medical Group 
Boca Raton, FL USA 
doctorb@baumanmedical.com 
baumanmedical.com 
linkedin/in/...
PRP Platelet Rich Plasma for Hair Regrowth by Dr. Alan Bauman
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PRP Platelet Rich Plasma for Hair Regrowth by Dr. Alan Bauman

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PRP Platelet Rich Plasma for Hair Regrowth by Dr. Alan Bauman

  1. 1. PLATELET RICH PLASMA FOR HAIR REGROWTH Alan J. Bauman, M.D. Diplomate, American Board of Hair Restoration Surgery PRP LifeLift™ Certification & Training September 13, 2014 ABHRS
  2. 2. CONFLICTS OF INTEREST • The following conflicts of interest pertain to this presentation: NONE
  3. 3. ALAN J. BAUMAN, M.D., A.B.H.R.S. • Diplomate, American Board of Hair Restoration Surgery • Member, International Society of Hair Restoration Surgery • Recommended, American Hair Loss Association • Member, American Academy of Anti-Aging Medicine (1998) • New York Medical College • Beth Israel Medical Center NY – General Surgery • Mt. Sinai Medical Center NY – General Surgery • Bauman Medical Group (est. 1997) - Boca Raton, FL • >17,000 patients treated, >7,000 hair transplants
  4. 4. VIDEO: PRP HAIR REGROWTH ON ABC http://hai.rs/hairprp-placenta
  5. 5. OVERVIEW • Hair Follicle & Growth Cycles • Hair Loss & Treatments • PRP & Growth Factors • PRP in Hair Restoration • Role of Extracellular Matrix (ECM) • ACell/Matristem & BioD-Restore • Hair Evaluations & Measurements • How to Prepare & Apply Scalp PRP • Additional Educational Opportunities
  6. 6. HAIR & SKIN ANATOMY: THE PILOSEBACOUS UNIT
  7. 7. HAIR GROWTH CYCLE
  8. 8. HAIR FOLLICLE STEM CELL SIGNALING • Epithelial Wnt/β-catenin signaling is required for hair matrix cell proliferation. Wnt/β-catenin signaling is a central regulator of adult stem cells. • LRP inhibition permits stem cell survival; blocks hair growth reversibly • β-catenin is required in bulge stem cells for their proliferation, but not survival indicating the persistence of functional progenitors so follicles will continue to proliferate even without β- catenin. • Hair Follicle ~ Fibroblast “Cousin”
  9. 9. Hair Loss • Fear/anxiety ~ Sickness or Illness • Low Self-Esteem/Self-Confidence • Feel Older / Less Attractive • Camouflage/Compensate • Avoid Social Situations
  10. 10. HAIR LOSS: ANDROGENETIC ALOPECIA (AGA) • Genetic Male and Female Pattern Hair Loss – most common type • Affects nearly 100 Million Americans (est. 60M men, 40M women)
  11. 11. 60X & 30X “HAIRCAM” DERMOSCOPY NORMAL MINIATURIZED
  12. 12. SEVERE DEPLETION: HAIR TRANSPLANTATION
  13. 13. HAIR LOSS: ALOPECIA AREATA (AA) • Autoimmune • Small / Circumscribed • “Spot baldness” • 1-2% of cases spreads to the entire scalp • Standard Tx’s: • Steroid injections • Microtrauma
  14. 14. MEDICAL HAIR LOSS TREATMENTS: PRESERVE AND ENHANCE HAIR FOLLICLE FUNCTION MODAL ITY TX MECHANISM Oral Finasteride blocks Dihydrotestosterone production [♂AGA] Topical Minoxidil K+ channel opener, vasodilator [AGA, AA] Phototherapy LLLT, LaserCap ↑ mitochondrial membrane potential [AGA, AA] Nutraceutical Biotin, Marine Protein Metabolic/Nutritional [AGA, AA] Cell Therapy Platelet Rich Plasma Growth Factors, Cytokines [AGA, AA]
  15. 15. PLATELET RICH PLASMA FOR HAIR REGROWTH
  16. 16. GROWTH FACTORS, CYTOKINES AND MORE… • platelet-derived growth factor - PDGF • transforming growth factor beta - TGF-B • fibroblast growth factor • insulin-like growth factor 1 - IGF1 • insulin-like growth factor 2 - IGF2 • vascular endothelial growth factor - VEGF • epidermal growth factor - EGF • nerve growth factor - NGF • interleukin-1 – IL1-B • Interleukin-8 – IL8 • keratinocyte growth factor • connective tissue growth factor
  17. 17. GROWTH FACTORS & HAIR FOLLICLE CYCLING • PDGF – stimulate growth of dermal mesenchyme, epidermis-follicle interaction (5) • VEGF – mitotic & endothelial cells, increases vascular permeability (Yano-6) • EGF – mitosis of epithelial cells, fibroblasts. Inhibits catagen, promotes anagen(10/11) • IGF – anti-apoptosis (12) • FGF – proliferation & differentiation of keratinocytes and endothelial cells. Anagen (13) • NGF – hair cycle modulator. Stimulates hair growth (NGF-TrkA) via proliferation of keratinocytes, NGF-p75NTR promotes apoptosis and hair follicle regression. Stress mediator, may play a role in neurogenic inflammation, hair follicle apoptosis & premature catagen.
  18. 18. PLATELET RICH PLASMA STAND-ALONE TREATMENT FOR HAIR REGROWTH • Autologous (or Allograft) • Bedside • Out-patient • Quick; “lunch-hour” • Non-Invasive • Painless Local Anesthesia • No Downtime • Low risk • Hair Growth www.HairCoach.net YesPRP at Bauman Medical
  19. 19. VEGF VASCULAR ENDOTHELIAL GROWTH FACTOR Bouhanna/Amgar
  20. 20. LITERATURE REVIEW: USE OF PRP IN HAIR RESTORATION • Graft-storage media in Hair Transplantation (Bouhanna/Amgar) • Wound healing accelerator in Donor and Recipient Areas (R Reese, J Cole, J Cooley) • Improvement of ‘take-rate’ & growth in hair transplantation (R Reese, C Uebel) • Stand alone treatment for androgenetic alopecia or alopecia areata. (J Greco, Amgar, Bouhanna, Rinaldi) Bouhanna/Amgar
  21. 21. PRP FOR ANDROGENETIC ALOPECIA Bouhanna/Amgar
  22. 22. PRP FOR ALOPECIA AREATA PreTx 8 weeks 4 months
  23. 23. PRP FOR ALOPECIA AREATA PreTx 8 weeks 4 months
  24. 24. PRP HAIR REGROWTH IN 38YO FEMALE AGA BEFORE & AFTER 9MOS
  25. 25. PRP HAIR REGROWTH 36YO MALE AGA BEFORE & AFTER 9MOS
  26. 26. EXTRACELLULAR MATRIX (ECM) • Extracellular part of multicellular structure • Structural & Biochemical support • Injury Repair & Tissue Growth • Wound healing / fibrosis • Regulates intracellular communications FUNCTIONS during injury or tissue regeneration: • Prevents inflammation & scar tissue formation • Facilitates tissue repair
  27. 27. WHY USE EXTRACELLULAR MATRIX (ECM) W/ PRP? PROLONG & ENHANCE! • Without ECM: PRP effects last ~90 days, requiring repeat treatments • With ECMs • PRP hair growth effects may last for up to 2 years • PRP hair growth effects appear to be stronger
  28. 28. ACELL/MATRISTEM ECM • Porcine Bladder Matrix • Micronized powder • Collagen • Basement membrane • Bio-scaffold
  29. 29. BIOD-RESTORE - PLACENTAL TISSUE ECM BioD-Restore™ is a cryopreserved amnion allograft from the placenta of live, healthy donors. • Immune privileged - Rarely evokes immune response • Provides cells, structure and signals for repair/regeneration • Natural scaffold / ECM for cell migration & proliferation • Proteins, cytokines, carbohydrates, hyaluronic acid, growth factors, chemical compounds and multipotent cells naturally inherent in placental and amnionic fluid • Anti-inflammatory / Anti-microbial / Anti-scarring / Anti-adhesive
  30. 30. BIOD-RESTORE • Multipotential Tissue Matrix • Structural allograft derived from human amnion • Chemical compounds, growth factors, residual cells • Regeneration • Immune privileged • Multipotent cells
  31. 31. BIOD-RESTORE PROCUREMENT & PROCESSING: • Prescreened donors • Medical review • Prenatal testing • Behavioral risk assessment • Blood tests • Medical history • Disease testing • Aseptic recovery / sterile cesarean section • FDA-registered tissue bank • FDA-registered processing center
  32. 32. GROWTH FACTORS FOUND IN AMNION • Platelet-Derived Growth Factor (PDGF) • Transforming Growth Factor Beta (TGF-β) • Vascular Endothelial Growth Factor (VEGF) • Fibroblast Growth Factor (FGF) • Epidermal Growth Factor (EGF) • Insulin-like Growth Factor (IGF-1)
  33. 33. HAIR MEASUREMENTS & EVALUATIONS • “HairCheck” – Cross-Section Hair Bundle Trichometry • HMI – Hair Mass Index • Sensitive, scientific, repeatable, accurate • “HairCam” Dermoscopy – identifies miniaturization • Global Photos – medical ‘mugshots’ HairCam – ABC Good Morning America HairCheck at Bauman Medical
  34. 34. HOW TO PREPARE AND APPLY PRP+ECM • Patient Selection, treatment area identified & measured. • Phlebotomy is performed. • Scalp prepped w/ antiseptic shampoo and local anesthesia. • PRP is prepared using centrifuge & YesPRP™ kit. • If used, BioD-Restore is thawed. • PRP is mixed gently with ECM (Acell/Matristem or BioD-Restore) • PRP+ECM is injected intradermally into the scalp • Mechanical (MicroPen) microneedling creates microtrauma • Scalp is cleansed, 15min LLLT applied • instructions given prior to discharge Dr. Bauman performs PRP on ABC Palm Beach
  35. 35. SIDE EFFECTS / SEQUELAE / FOLLOW-UP • Tenderness / Swelling is rare • Follow-ups can be performed monthly to track hair growth improvements. • Monitor hair growth using HairCheck and HairCam to observe peak and plateau.
  36. 36. UPCOMING PRP HAIR GROWTH & HAIRCHECK™ WORKSHOPS Friday September 19th -or- Friday November 14th 9am-5pm Boca Raton • Hair Loss Diagnosis & Evaluations, Treatments & Tracking • Hands-on HairCheck Measurements and Hands-on PRP+ECM hair regrowth procedure • Course Director: Alan J. Bauman, M.D. • Certificate & Starter Kit • Email: andres@baumanmedical.com • www.HairCoach.net for info/registration and future events
  37. 37. REFERENCES Marx, R.E. , et al, “Platelet-Rich Plasma Growth Factor Enhancement for Bone Grafts,” Oral Surg Oral Med Oral Patrhol, 1998;85:638-646. Antonaides, H.N., et al,”Human Platelet-Derived Growth Factor: Structure and Functions”, Federation Proceedings, 1983;42:2630-2634. Pierce, G.F., et al,”PDGF-BB,TGF-β 1 and Basic FGF in Dermal Wound Healing: Neovessel and Matrix Formation and Cessation Repair”, Am J Pathology, 1992;140:1375-1388. ELISA Immuno Assay For PDGF-A, Human VEGF, TGF-β 1, and Human EGF, R&D Systems, Mpls, MN 1999. Bauman AJ, The use of cross-sectional hair bundle trichometry in the diagnosis, evaluation and tracking of hair loss. Poster Presentation. ISHRS ASM October 2012 Nassau, Bahamas. Gruss, J., et.al., Human amniotic membrane: a versatile wound dressing. CMA Journal 1978; Vol.118:1237-1246 De Coppi, P. et.al., Isolation of amniotic stem cell lines with potential for therapy. Nature Biotechnology 2007; Vol.25,No.1:1274-1280
  38. 38. PRP VIDEO PLAYLIST http://hai.rs/prphairgrowthvids
  39. 39. Alan J. Bauman, M.D. Bauman Medical Group Boca Raton, FL USA doctorb@baumanmedical.com baumanmedical.com linkedin/in/alanjbauman twitter.com/DrAlanBauman facebook.com/alanjbauman

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