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Summary. Phaeomoniella chlamydospora is an important tracheomycotic fungus involved in different syndromes
of the esca disease complex affecting young and mature grapevine plants. Although grapevine planting material
is frequently infected by this pathogen, no regular screening to detect the fungus is carried out, mainly due to
the lack of fast, sensitive and affordable methods for large-scale application. A monoclonal antibody-based triple
antibody sandwich (TAS) enzyme-linked immunosorbent assay (ELISA) was developed to specifically detect Pa.
chlamydospora from grapevine wood tissues. This assay could be especially useful for large-scale application in
nurseries, to ensure Pa. chlamydospora-free grapevine stocks, and thus contributing to the production of healthy
propagation material.
DOI: 10.14601/Phytopathol_Mediterr-11860Development of a monoclonal antibody TAS-ELISA assay for detection of Phaeomo...
Development of a monoclonal antibody TAS-ELISA assay for detection of Phaeomo...IHMT-UNL, GHTM, Medical Parasitology
Recommended
Summary. Phaeomoniella chlamydospora is an important tracheomycotic fungus involved in different syndromes
of the esca disease complex affecting young and mature grapevine plants. Although grapevine planting material
is frequently infected by this pathogen, no regular screening to detect the fungus is carried out, mainly due to
the lack of fast, sensitive and affordable methods for large-scale application. A monoclonal antibody-based triple
antibody sandwich (TAS) enzyme-linked immunosorbent assay (ELISA) was developed to specifically detect Pa.
chlamydospora from grapevine wood tissues. This assay could be especially useful for large-scale application in
nurseries, to ensure Pa. chlamydospora-free grapevine stocks, and thus contributing to the production of healthy
propagation material.
DOI: 10.14601/Phytopathol_Mediterr-11860Development of a monoclonal antibody TAS-ELISA assay for detection of Phaeomo...
Development of a monoclonal antibody TAS-ELISA assay for detection of Phaeomo...IHMT-UNL, GHTM, Medical Parasitology
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