Covalent Versus Electrostatic Attachment of Yeast Cytochrome c to a Fused Si...smistry_us
1. The document discusses the covalent and electrostatic attachment of yeast cytochrome c to a fused silica surface and how its structure and function are affected.
2. Experiments examined how the protein's absorption spectra changed when covalently or electrostatically attached to the surface versus in solution under varying conditions like pH and alcohol concentration.
3. The goal was to better understand how the attachment method and external factors influence the protein's conformation and ability to perform its electron transport function.
Study of the Folding and Unfolding of Proteins Adsorbed to a Fused Silica Sur...smistry_us
This document summarizes a study on the folding and unfolding of cytochrome c proteins adsorbed to a fused silica surface. It introduces cytochrome c, describes solution and surface spectroscopy experiments, and compares experiments using horse and yeast cytochrome c. Specifically, it examines how horse cytochrome c unfolds with increasing alcohol concentration in solution versus on the surface, finding proteins are more protected from denaturation when adsorbed. Similar experiments with yeast cytochrome c show it takes longer to unfold on the surface than in solution.
Covalent Versus Electrostatic Attachment of Yeast Cytochrome c to a Fused Si...smistry_us
1. The document discusses the covalent and electrostatic attachment of yeast cytochrome c to a fused silica surface and how its structure and function are affected.
2. Experiments examined how the protein's absorption spectra changed when covalently or electrostatically attached to the surface versus in solution under varying conditions like pH and alcohol concentration.
3. The goal was to better understand how the attachment method and external factors influence the protein's conformation and ability to perform its electron transport function.
Study of the Folding and Unfolding of Proteins Adsorbed to a Fused Silica Sur...smistry_us
This document summarizes a study on the folding and unfolding of cytochrome c proteins adsorbed to a fused silica surface. It introduces cytochrome c, describes solution and surface spectroscopy experiments, and compares experiments using horse and yeast cytochrome c. Specifically, it examines how horse cytochrome c unfolds with increasing alcohol concentration in solution versus on the surface, finding proteins are more protected from denaturation when adsorbed. Similar experiments with yeast cytochrome c show it takes longer to unfold on the surface than in solution.