This letter provides a reference for Nilabh Ranjan, who worked as a PhD student in Prof. Dr. Karlheinz Friedrich's research group from 2012-2015. The letter summarizes that Nilabh pursued a project on the cytokine TSLP involved in asthma, developing a cellular assay and generating recombinant proteins that inhibited TSLP activity. Nilabh acquired various laboratory skills and recently submitted his PhD thesis. Throughout his work, Nilabh overcame challenges with perseverance. He was dedicated to the research group and helped organize a summer school, enthusiastically assisting international participants. Prof. Friedrich believes Nilabh will be an asset to any future position due to his skills, enthusiasm,
From post-doc to present day.
Presentation for the NIHR CLAHRC National Conference Careers Day: for postdoc research careers in the allied health professions
From post-doc to present day.
Presentation for the NIHR CLAHRC National Conference Careers Day: for postdoc research careers in the allied health professions
One of the books, published when I was Founding Director and General Manager at SingHealth Academy.
It chronicles stories about Blood Stem Cell Transplants at SingHealth and the excellent work of healthcare professionals, the ecosystem of support for patients and the journeys of patients.
The Spychala’s Great Discoveries in Science: Works of GeniusJaroslaw Spychala
Dr. Jaroslaw Spychala is an internationally visible researcher with an excellent record of accomplishment in the area of synthetic organic chemistry and synthetic methods. The talented and motivated individual has shown a great sense of commitment to education and research to realize academic aspirations. His many outstanding performances (particular knowledge, scientific excellence within amidines and amides, sophisticated experimental work) have been published in high quality journals: Spychala Reaction I (secondary amides and cyclic amidines from nitriles, the reaction regarded mistakenly as the Thio-Pinner Reaction), Spychala Reaction II (simple amidines from thioamides and zinc ammoniates), Spychala Reaction III (several protonation reactions and reactivity, strong chelation of protons, and zwitterionic monomethylamidine formation), Spychala Rearrangement I (H2S molecule migration in the thioamide-nitrile system), Spychala Rearrangement II (conformational rearrangement during deprotonation, hydrogen-bonding capabilities of modified amide groups), newfound bonds (N…H+…N HBs, the H+-bonding strength can approach that of covalent bonds), correlations (1H-15N spin-spin interactions, unique spectroscopic properties), chemical reactivity (HB as a driving force for desired selectivity accelerating the transformation of the starting materials towards one stereoisomer, a new spectroscopic approach to), high levels of selectivity (stereochemistry, site selective and stereoselective reactions, geometrical stereoisomers, and predictable stereoselectivity), stable hydrogen-bonding systems as a driving force in synthesis (HB assisted activation and product stabilization), new multicomponent hydrogen-bonded systems (e.g. with dihydrogen bonds), new multifurcated ionic hydrogen bonds (cooperative hydrogen bonding), and other individual valuable contributions to organic chemistry and medicinal drug discovery. The positively charged linkages and reactions discovered are of fundamental importance. One of the modes of action of anticancer compounds is the targeting of the DNA of cancer cells (scientifically viable anticancer strategy investigated and verified). Thus, some basic interaction studies of amidines and amides are of great relevance. The achievements he is most proud of cover INDIVIDUAL SUCCESS. The superior top publications describe the pioneering, world-class research. www.ncbi.nlm.nih.gov/myncbi/jjspychala/cv/490709/
Creativity, persistency, accuracy, and a broad interest for techniques beyond traditional organic chemistry are essential for success. Published full papers are highlighted on Orcid, NCBI, Prabook, Publons, and Research Gate pages to enhance cutting-edge research visibility to the field of Chemistry and Bioorganic Chemistry for dissemination of primary research. These are the top 15 monoauthored research works; own scientific discoveries at the excellent weblinks (https://mostwiedzy.pl/pl/jaroslaw-spychala,220731042045745-0).
One of the books, published when I was Founding Director and General Manager at SingHealth Academy.
It chronicles stories about Blood Stem Cell Transplants at SingHealth and the excellent work of healthcare professionals, the ecosystem of support for patients and the journeys of patients.
The Spychala’s Great Discoveries in Science: Works of GeniusJaroslaw Spychala
Dr. Jaroslaw Spychala is an internationally visible researcher with an excellent record of accomplishment in the area of synthetic organic chemistry and synthetic methods. The talented and motivated individual has shown a great sense of commitment to education and research to realize academic aspirations. His many outstanding performances (particular knowledge, scientific excellence within amidines and amides, sophisticated experimental work) have been published in high quality journals: Spychala Reaction I (secondary amides and cyclic amidines from nitriles, the reaction regarded mistakenly as the Thio-Pinner Reaction), Spychala Reaction II (simple amidines from thioamides and zinc ammoniates), Spychala Reaction III (several protonation reactions and reactivity, strong chelation of protons, and zwitterionic monomethylamidine formation), Spychala Rearrangement I (H2S molecule migration in the thioamide-nitrile system), Spychala Rearrangement II (conformational rearrangement during deprotonation, hydrogen-bonding capabilities of modified amide groups), newfound bonds (N…H+…N HBs, the H+-bonding strength can approach that of covalent bonds), correlations (1H-15N spin-spin interactions, unique spectroscopic properties), chemical reactivity (HB as a driving force for desired selectivity accelerating the transformation of the starting materials towards one stereoisomer, a new spectroscopic approach to), high levels of selectivity (stereochemistry, site selective and stereoselective reactions, geometrical stereoisomers, and predictable stereoselectivity), stable hydrogen-bonding systems as a driving force in synthesis (HB assisted activation and product stabilization), new multicomponent hydrogen-bonded systems (e.g. with dihydrogen bonds), new multifurcated ionic hydrogen bonds (cooperative hydrogen bonding), and other individual valuable contributions to organic chemistry and medicinal drug discovery. The positively charged linkages and reactions discovered are of fundamental importance. One of the modes of action of anticancer compounds is the targeting of the DNA of cancer cells (scientifically viable anticancer strategy investigated and verified). Thus, some basic interaction studies of amidines and amides are of great relevance. The achievements he is most proud of cover INDIVIDUAL SUCCESS. The superior top publications describe the pioneering, world-class research. www.ncbi.nlm.nih.gov/myncbi/jjspychala/cv/490709/
Creativity, persistency, accuracy, and a broad interest for techniques beyond traditional organic chemistry are essential for success. Published full papers are highlighted on Orcid, NCBI, Prabook, Publons, and Research Gate pages to enhance cutting-edge research visibility to the field of Chemistry and Bioorganic Chemistry for dissemination of primary research. These are the top 15 monoauthored research works; own scientific discoveries at the excellent weblinks (https://mostwiedzy.pl/pl/jaroslaw-spychala,220731042045745-0).
1. Prof. Dr. Karlheinz Friedrich
Nonnenplan 2-4
07743 Jena
Telefon 03641-9396405
Telefax 03641-938642
E-Mail: karlheinz.friedrich@med.uni-jena.de
Jena, 09.07.2015
Universitätsklinikum Jena ·Institut für Biochemie II · Postfach · 07740 Jena
Bachstraße 18 · 07743 Jena · Telefon 03641 93 00 Universitätsklinikum Jena · Körperschaft des öffentlichen Rechts
als Teilkörperschaft der Friedrich-Schiller-Universität Jena
Internet: www.uniklinikum-jena.de Verwaltungsratsvorsitzender: Prof. Dr. Thomas Deufel
Gerichtsstand Jena Medizinischer Vorstand und Sprecher des Klinikumsvorstands:
Steuernummer 161 / 144 / 02978 · USt.-IdNr. DE 150545777 Prof. Dr. Klaus Höffken
Bankverbindung: Wissenschaftlicher Vorstand: Prof. Dr. Klaus Benndorf
Sparkasse Jena · BLZ 830 530 30 · Konto 221 Kaufmännischer Vorstand: Dr. Brunhilde Seidel-Kwem
Institut für Biochemie II
To whom in may concern
Reference for Nilabh Ranjan
Nilabh Ranjan has been working as a PhD student in my research group since January 2012
and was funded throughout most of this period of time by a scholarship granted by the
Interdisciplinary Center for Clinical Research (IZKF) Jena.
Nilabh has pursued a project concerned with activity and inhibition of murine Thymic Stromal
Lymphopoietin (TSLP), a cytokine involved in the pathogenesis of Asthma bronchiale. He has
first adopted a cellular readout system for TSLP activity based on heterologous expression of
the TSLP receptor in a murine cell line in combination with a luciferase reporter gene. Using
published structural and functional information, he produced a recombinant murine TSLP
mutant with an amino acid substitution which he proved to interfere with the TSLP-induced
cellular response and to act as a partial antagonist. He also generated a recombinant ligand-
binding domain of the TSLP receptor which specifically blocked TSLP activity on the model
cells. Finally, Nilabh was able to show that the two proteins were potent in blocking a
physiological TSLP function, namely the upregulation of inflammatory surface markers on
natural murine dendritic cells. These achievements open up the perspective to evaluate the
performance of the TSLP inhibitors in a murine Asthma model in the future and were the basis
for a PhD thesis entitled „A study on antagonistic targeting of thymic stromal lymphopoietin
(TSLP) as a potential therapeutic strategy for Asthma bronchiale” which Nilabh has recently
submitted for evaluation by the Medical Faculty of this University.
Throughout his experimental work, Nilabh has acquired and successfully applied a wide variety
of biochemical and cell biological methods ranging from recombinant DNA work through cell
cultivation, flow cytometry to protein analysis and purification. His recently obtained results are
convincing and very interesting and his experimentation has reached a quality level that should
allow for the imminent submission of a conclusive paper to a major scientific journal.
2. In the course of his stay in my laboratory, Nilabh’s capabilities have progressively developed in
a remarkable manner. Being very ambitious and determined, he pursued his project patiently
and overcame setbacks with great energy and perseverance. He never lost faith in himself, was
very active and creative in finding useful information in the literature and persistently seeked
advice from experienced colleagues. He e.g. made consequent and successful use of
suggestions by his thesis committee to whom he reported progress annually. As a result of his
efforts, he has also acquired decent skills in structuring scientific information, planning novel
experiments, giving presentations and compiling scientific manuscripts.
As a group member, Nilabh has been very dedicated, sincere and dependable. I particularly
appreciate his ability to enthuse positive energy and team spirit among other team members.
For instance, he has played a vital role in the organization of last years’ “Summer Schools on
Molecular Medicine” at the University of Jena and has joyfully volunteered to assist the
international participants in finding their way and feeling comfortable in Jena during their stay.
His devotion and creativity as a “mentor” during this time was exceptional.
I understand that Nilabh is now applying for a future position. I am convinced that he will be a
major gain for any working environment in which he can contribute his scope of skills, his
enthusiasm and his constant will to still further unfold his talents. I wish him all the best for his
future.
Prof. Dr. KH. Friedrich