2. Overview of microbiology
Microbial diversity
• Bacteria
• Archaea
• Fungi
• Algae
• Protozoa
• Branches of microbiology
History of microbiology
3. Branch of biology that studies microorganisms
Microorganisms or microbes- minute living things that
individually are too small to be seen with unaided eye
7. Bacteria (singular: bacterium) are relatively
simple, single-celled (unicellular) organisms.
Prokaryotes- genetic material enclosed in a
special nuclear membrane (Greek word- pre-
nucleus)
Cell wall- peptidoglycan
Several shapes:
Round, Rod like
Spherical or ovoid
Spiral
8. Archaea:
• Prokaryotes
• They have cell wall but wall lack peptidoglycan.
• Extreme environments- Hot springs, hot sulfurous water
Fungi:
• Eukaryotes
• Unicellular (yeast) or multicellular (molds, mushrooms)
• Cell wall chitin
Protozoa:
• Unicellular eukaryotic
• Move by pseudopodia, flagella, cilia
• E.g. Amoeba, Euglena
Algae:
Photosynthetic eukaryotes
Cell wall- cellulose
Viruses:
So small- electron microscope
Acellular (not cellular)
Protein coat
Nucleic acid (either DNA or RNA)
9. Beneficial
Help maintain balance of living organisms and chemicals in environment
Soil microbes- help breakdown wastes and incorporate nitrogen gas from
air
Play role in photosynthesis- food generating process
Intestinal microbes- help in digestion + synthesis of some vitamins
e.g vitamin B for metabolism, vitamin K for blood clotting
Synthesis of certain chemical products- acetone, alcohols, organic acids,
enzymes, many drugs
Food industries- cheese, yogurt, pickles, alcoholic beverages
Harmful
Pathogens, disease causing, spoilage of food
10. Bacteria are primary decomposers - recycle nutrients
back into the environment
Maintain the balance of living organisms and chemicals
in our environment
Marine and fresh water microorganisms-food chain
Soil microbes- breakdown waste and nitrogen cycle
photosynthesis
11. Microbes produce various food products
cheese, pickles, green olives
yogurt, soy sauce, vinegar, bread
Beer, Wine, Alcohol
Helps in digestion and synthesis of enzymes
Example: E. coli
B vitamins - for metabolism
Vitamin K - blood clotting
Microbes are used to produce Antibiotics
-Mold -Penicillium notatum (Penicillin)
12. Bacteria used to produce substances
-Cellulose
-
Microbial antagonism
-role of microbial flora
Insect Pest Control
-Using bacteria to control the growth of insects
-Bacillus thuringiensis
-caterpillars
13. Bioremediation
-Using microbes to clean up pollutants and toxic
wastes
-Pseudomonas sp.
-Bacillus sp.
Commercial Applications
-production of vitamins (B2 & B12),
-organics acids (lactic acid, acetic acid)
-enzymes (protease, lipase, peptidase)
-alcohol (acetone, butanol)
14. Recombinant DNA Technology, Gene Therapy, Genetic
Engineering
Bacteria can be manipulated to produce enzymes and
proteins they normally would not produce
Insulin
Human Growth Hormone
Interferon