The film The Human Centipede uses several horror conventions and film theories. It features a psychopathic doctor villain who surgically attaches 3 people mouth to anus, creating a "human centipede." This transgressive act subverts the normal positive iconography of doctors and hospitals. Feminist theory also applies as the 2 female victims are vulnerable and subjected to the male gaze. The ending leaves one victim alive, allowing for a potential sequel as theorized by Carroll. Overall the film creates horror through disturbing the expected meanings of common icons and empowering the villain over his victims.