Meghan Ashley Holder has over 10 years of experience in environmental engineering, laboratory analysis, and aquatic biology. She received a Bachelor's degree in Resource Biology from the University of Louisiana at Lafayette in 2010 and has worked for companies such as Tanner Services and the USGS. Her duties have included chemical sampling, laboratory maintenance, data analysis, and ensuring regulatory compliance. She provides 7 professional references who can speak to her work in areas like environmental remediation, fisheries research, and laboratory analysis.

1. Curriculum Vitae
Meghan Ashley Holder
1141 Madeline Heights
Breaux Bridge, LA 70517 US
Mobile: 985-518-8783; Email: meghanaholder@gmail.com
Work Experience:
Tanner Services/Environmental Division, LLC: 104 Abigayle's Row, Scott, LA 70583
Dates of employment: 12/2013 - 04/2016 - Salary: $60,000.00 USD/ year
Hours per week: 40 - Title: Environmental Engineer
Duties, Accomplishments and Related Skills: Chemical lab maintenance, in house experiments
with products and field materials; inspections of company facilities to ensure compliance with
state requirements on drinking water and NPDES standards; provide improvements and solutions
to current and possible problems with such facilities; field sampling in Texas and Louisiana thus
far which included traveling to sites to sample liquids and solids for oil and gas industry on
active, non-active, and possible superfund sites thought to be contaminated with heavy metals,
PCBs, PAHs, BTEX, TPH, Oil and Grease, NORM and other RCRA classified hazardous
wastes; author SOPs for field sampling, analytical test requirements respective to state codes, lab
techniques, and managing shipping logistics of samples and testing requirements with nationally
accredited analytical labs; written and electronic logs of locations and sample quality/quantity/
site description; generation of SOPs for recycling protocols used intra-company use as well as
via customer; prepare and present company power point presentations for advertising, pilot study
results, and site conditions; provide technical assistance in the modification and blending of
company products for optimal results, along with their application on site; supervising and
training employees in proper safety and application techniques; oversee de-watering, back filling
and closure projects; ensured company and customer compliance with state and federal
regulations in hazardous waste/exemption and disposal/recycle/remediation(Texas Railroad
Commission, LA-DNR, RCRA 40 CFR); generate, evaluate and approve reports for company
and customer(s) that would include explanations of analytical lab reports, sampling logs, state
and federal guideline compliance/non-compliance, and closure/remediation techniques; office
manager duties of filing, conversion of MSDS to SDS due to GHS requirement changes, writing
invoices/POs, chemical orders, supply orders, payments, handling per diem for employees,
generating job control costs/ earning reports, and payroll.
The Pool Guy, LLC: 204 Burgess Dr., Broussard, LA 70518
Dates of Employment: 10/2013 - 12/2013 ∙ Salary: $11.00 USD/ hour
Hours per week: 50(+) ∙ Title: Pool Tech
Duties, Accomplishments and Related Skills: Customer pool care, operations maintenance,
and minor repairs of pool pumps and filters; water chemistry testing with corrections; customer
supply and repair orders; and shop inventory.
Supervisor: James Hutchinson (337-303-9003)
Okay to contact this Supervisor: Yes
Page 1 of 7

2. Work Experience: (continued)
USGS (Five Rivers/AKIMA): 700 Cajundome Blvd., Lafayette, LA 70506
Dates of Employment: 06/2011 - 08/2013 ∙ Salary: $16.00 USD/ hour
Hours per week: 40 ∙ Title: Lab Analyst (contractor)
Duties, Accomplishments and Related Skills: Responsible for wet and dry lab maintenance;
logistics of shipping/receiving time sensitive fish tissue/organ samples; generating needed
buffers; lab safety and MSDS (at that time) logs; fish blood osmolality measurements;
processing of fish blood, eyes, testes, and sperm for microscopy and cytometery analyses;
interpretation and analysis of quantitative and qualitative microscopy; cytometry; animal care
(fishes, frogs, crawfish); hematological cell extraction and separation (via centrifuge) to stain for
microscopy; assistance in sample logging, processing, and analyzing data for a Natural Resource
Damage Assessment (NRDA) project; assistance in completing a state license for housing
Tilapia; sexing of Tilapia (Oreochromus nilotecus); assist in preparing documents needed for an
Institutional Animal Care and Use Committee (IACUC) permit; assist in preparing permit to
house endangered pallid sturgeon (Scaphirynchus albus); assist in extracting and process fluid
from eyes of both farmed and feral Asian carp for genome determination as diploid or triploid to
decipher population movements; analyze catostomid sperm for abnormalities and counts as an
indicator species of the Columbia river in the northwest USA; preparing stains for cell
identification; optimize staining procedures for pallid sturgeon blood; anaesthetizing and
extracting blood from several spp. of fish; culling and logging of lab animals used in
experiments; write and review documents for literature publications of results; arrange analytical
data for statistical analysis and graphic display; attend and present findings at regional and
national meetings for American Fisheries Society; aquatic system cleaning, maintenance,
structure repairs, design and installation of new air delivery system.
Supervisor: Dr. Jill A Jenkins (225-324-0416)
Okay to contact this Supervisor: Yes
University of Louisana, Lafayette / USGS: 700 Cajundome Blvd., Lafayette, LA 70506
Dates of Employment: 11/2010 - 06/2011 ∙ Salary: $13.00 USD/ hours
Hours per week: 20 to 30 ∙ Title: Lab Tech (intern)
Duties, Accomplishments and Related Skills: Chemical lab and wet lab maintenance and
animal care; qualitative and quantitative microscopy of fish sperm; generation of needed buffers;
osmolality of buffers and biological samples; generation of data spreadsheets/ logs; bleeding and
sperm expression of fish; culling and logging of lab animals used in experiments; lab safety
including MSDS organization and updates.
Supervisor: Dr. Jill A Jenkins (225-324-0416)
Okay to contact this Supervisor: Yes
Page 2 of 7

3. Work Experience: (continued)
University of Louisana, Lafayette / NSF: E. University Cir., VLW410, Lafayette, LA 70503
Dates of Employment: 01/2010 - 12/2010 ∙ Salary: $11.00 USD/ hour
Hours per week: 30 ∙ Title: Research Education for Undergraduates (intern)
Duties, Accomplishments and Related Skills: National Science Foundation-Research
Education for Undergraduates (REU) intern; analyzed fish bio-diversity data from two
expeditions to southeastern Peru; amazonian fish identification, staining, photography, and
preservation; prepared and presented poster publication at a university and regional level
concerning the fish fuana diversity and richness within an area; aided in graphics for publications
within and outside the university; lab animal care; aided in the design and construction of web
pages for "Proyecto Alto Purus." (http://www.ucs.louisiana.edu/~jxa4003/Alto%20Purus.html).
Supervisor: Dr. James S. Albert (337-482-6627)
Okay to contact this Supervisor: Yes
South Lousiana Community College: 1101 Bertrand Dr., Lafayette, LA 70506
Dates of Employment: 07/2009 - 12/2010 ∙ Salary: $11.00 USD/ hour
Hours per week: 30 ∙ Title: Math and Science Tutor
Duties, Accomplishments and Related Skills: Tutor students in multiple aspects of math and
science including: basic math, algebra, physics, calculus, chemistry, anatomy, and biology.
Supervisor:Heather Maira
Okay to contact this supervisor: No
University of Louisana, Lafayette / USGS: 700 Cajundome Blvd., Lafayette, LA 70506
Dates of Employment: 09/2009 - 11/2010 ∙ Salary: $11.00 USD/ hour
Hours per week: 30 ∙ Title: Field sampler (intern)
Duties, Accomplishments and Related Skills: Tag and recapture of green tree frogs (Hyla
cinerea); input of alphanumeric tag information and animal stat data in a electronic program for
statistical analysis, was part of an an ongoing population dynamics and movement study.
Supervisor: Dr. Jacoby Carter (337-781-4882)
Okay to contact this Supervisor: Yes
Cricket's Pool World: 1100 Rue Du Belier, Lafayette, LA 70506
Dates of Employment: 06/2009 - 09/2009 ∙ Salary: $11.00 USD/ hour
Hours per week: 40 ∙ Title: Pool Tech
Duties, Accomplishments and Related Skills: In store customer service; water chemistry
testing and generate recommendations for corrections. Handling of funds and end of day
deposits.
Supervisor: Katherine or Scott Benoit (337-984-7626)
Okay to contact this Supervisor: Yes
Page 3 of 7

4. Work Experience: (continued)
City of Clearwater, Florida: 900 N. Martin Luther King Blvd., Clearwater, FL 33755
Dates of Employment: 10/2007 - 06/2009 ∙ Salary: $24,000.00 USD/year
Hours per week: 40 ∙ Title: Aquatics Progmmer
Duties, Accomplishments and Related Skills: Customer service at the North Greenwood
Recreational Facilty; water chemistry and correction/ammendments needed for public use of
swimming pools; attend National Recreation and Parks Association conferences and trainings for
Aquatic Progammers; teach and maintain certifications for safety, NIMS (FEMA) training,
lifeguarding, AED, CPR, first aid, and First Repsonder courses; supervise a staff of 12 lifeguards
during 3/4 of the year; manage camp attendance dynamics; manage major repairs/improvements;
perform minor repairs/maintenance which included regulary backwashing, chemical orders,
safety product orders; correspondence with other City of Clearwater aquatic/recreation sites;
generate end of year profit reports and attendance logs; create and supervise festivity events;
comptroll city wide events; manage/supervise swim team practices and events; coordinate and
teach swim lessons (multi-level); received employee of the month within first six months of
employment (among 10 employees), received employee of the year within first year of
employment (among 300 employees); fill in at multiple city recreation/pool/nature park sites.
Supervisor: Barbara Moon/ Kathy LaTerza/ Mark Roberson (727-462-6276)
Okay to contact this Supervisor: Yes
Education:
University of Louisiana, Lafayette: Lafayette, LA
Baccalaureate’s Degree 12/2010
GPA: 3.14 of a maximum 4.0
Credits Earned: 46 Semester hours
Major: Resource (Marine) Biology/ biodiversity Minor: Chemistry
Relevant Coursework, Licenses and Certifications: biological diversity, microbiology,
ichthyology, organic chemistry I and II with labs, environmental toxicology, physiology, physics
II (electricity/currents)
Honors: Dean's List
University of South Florida, Tampa: Tampa, FL
Coursework Completed towards Baccalaureate’s degree
Credits Earned: 21 Semester hours
Major: Marine Biology
Relevant Coursework, Licenses and Certifications: global conservation, wetland
environments, physics I with lab, marine biology, principles of ecology with lab
Page 4 of 7

5. Education: (continued)
St. Petersburg Community College: Saint Petersburg, FL
Associate's Degree 06/2007
Credits Earned: 50 Semester hours
Major: Visual Arts
Relevant Coursework, Licenses and Certifications: Fundamentals of Biology, qualitative and
analytical chemistry II with lab; ceramics I and II, drawing I and II, design I
Eckerd College: Gulfport, FL
Coursework Completed towards Baccalaureate’s degree
Credits Earned: 66.5 Semester hours
Major: Marine Biology Minor: Chemistry
Relevant Coursework, Licenses and Certifications: introduction to marine science, marine
geology, genetics, cell biology, oceanography, mathematical modeling, Calculus I and II,
independent research of cownose ray migration (Rhinoptera bonasus), marine invertebrate
biology, general Chemistry I with lab
Job Related Training:
∙ Fork lift operation
∙ PEC safety basic (includes SafeLand and SafeGulf)
∙ PADI open water diver certification
CEU credits for the following attendences:
"Remediaton with Nano-Zero Valent Iron (nZVI): Recent Developments,
Applications, and Global Perspectives." at the Ninth International Conference on
Remediation of Chlorinated and Recalcitrant Compounds." May 18-22, 2014;
Monterey, CA.
"Soil and Groundwater Remediation Technology Seminar." November 12, 2015;
Baton Rouge, LA.
Language Skills:
Language Spoken Written Read
English Advanced Advanced Advanced
Spanish-
American
Novice Novice Novice
Page 5 of 7

6. Affiliations:
∙ American Fisheries Society - Previous member
∙ National Parks and Recreation Association - Previous member
∙ Eckerd College Search and Rescue (EC-SAR) - Previous student member
∙ Institution Animal Care and Use Committee (IACUC) - Current community review
member of the Wetlands Research Center in Lafayette, LA
Professional Publications:
"Improved Peripheral Blood Extraction and Staining Protocols Modified for Pallid Sturgeon
(Scaphirynchus albus)." (in review) Applied Ichthyology; National Wetlands Research
Center, Lafayette, LA. Authors: Meghan A Holder, Jill A. Jenkins, Tiffany S. Smoak,
Rassa Draugilas O-Dale.
"An Improved Protocol for Hematology Staining with Pallid Sturgeon." (Poster) 2013- Presented
at the National American Fisheries Society meeting, September 8-12, 2013; Little Rock,
AR. Authors: Meghan A. Holder (1), Tiffany S. Smoak (2), Jill A. Jenkins (3).
"Comparisons of Biomonitoring Results from Davis Pond and Caernarvon Mississippi River
Freshwater Diversions." (Poster) 2012- Presented at the Southeastern Division American
Fisheries Society meeting, January 2-29, 2012; Biloxi, MS. Authors: Heahter Olivier (1),
Beth B. Bourgeois (2), Paul J. Conzelmann (3), Meghan A. Holder (4), Rassa Draugelis-
Dale (4), Jill A. Jenkins (4).
"Fishes of the Fitzcarrald, Peruvian Amazon." (Illustrations in Book) 2012- Lulu Press; Releigh,
NC. Authors: J.S. Albert, T.P. Carvalho, J.A. Chuctaya, P. Petry, R. E. Reis, B. Rengifo,
and H. Ortego.
"Aquatic Bio-Diversity in the Amazon: Habitat Specialization and Geographic Isolation Promote
Species Richness." (article) 2011 -Animals, 1 (2), 205 -241; doi:10.3390ani1020205.
Authors: James S. Albert, Tiago P. Carvalho, Paulo Petry, Meghan A. Holder, Emmanuel
L. Maxime, Jessica Espino, Isabel Corahua, Roberto Quispe, Blanca Rengifo, Hernan
Ortega, and Roberto E. Reis.
"Aquatic Bio-diversity in the Amazon: Headwater Fish Fauna of the Fitzcarrald Arch." (poster)
2010 - Presented at the Graduate Student Symposium, University of Louisiana, Lafayette
in Lafayette, LA; 2011 - Presented at the Southeastern Evolutionary and Ecology
Conference in Auburn, AL; Authors: Meghan A. Holder, Tiago P. Carvalho, and James
S. Albert.
Page 6 of 7

7. Professional Publications: (continued)
"Tag Retention of the New Model of Alpha-Tags from Northwest Marine Technology in Green
and Squirrel Treefrogs (Hyla cinerea and H. squirella): Stiffer Tags Cause Many More
Problems." (Poster) 2010- Presented at The Ernie Liner Biennial Herpetological Research
Group Meeting, November 19-21, 2010; LUMCON Cocodrie, LA. Authors: Jacoby
Carter, Jeromi Hefner, Meghan Holder, Forest Huval, Kelsie Hayes, Arianne Martin and
Lance Renoux.
References:
Name Employer Title Phone/ E-mail
Fadi Raad Tanner Services Field Superintendent 956-607-6986
fraad@tannerservices.net
James S. Albert (*) University of Louisiana Professor of Biology 337-482-6627
Lafayette jalbert@louisiana.edu
Jeromi Hefner Acadian Ambulance IT specialist 337-247-0544
Jeromi.hefner@gmail.com
Lance Renoux Ocean Associates, Inc. Fisheries Biologist II 337-349-8584
Lance.renoux@gmail.com
Dr. Jill A. Jenkins (*) USGS Senior Research Scientist 225-324-0416
jenkinsj@usgs.gov
Dr. Jacoby Carter (*) USGS Research Ecologist 337-781-4882
carterj@usgs.gov

8. Page 7 of 7