Maracaibe is a city located in Venezuela. It is the capital and largest city of Zulia State. Maracaibe has a population of over 4 million people and is Venezuela's second largest metropolitan area after Caracas.
The document discusses key aspects of Islam including its founder Mahoma and important locations in Mecca such as the Kaaba. Islam was founded by the prophet Mahoma in the 7th century and centers around belief in one God. The most important site in Islam, the Kaaba, is located in Mecca and is the direction of prayer for Muslims around the world.
Recife Antigo is the historic center of Recife, Brazil. It is located along the Capibaribe River and features well-preserved colonial architecture from the 16th-17th centuries including churches, monasteries, and mansions built with coral stone. The area offers a glimpse into Recife's past and is a popular tourist destination known for its culture and architecture.
Olinda and its pre-carnival celebrations are a major tourist attraction in Brazil's northeastern state of Pernambuco. For over a month leading up to carnival, the historic city of Olinda holds daily street parties and concerts to get revelers in the mood for the big event. These festivities showcase Olinda's rich cultural heritage through music, dance, costumes and colorful parades.
O Rio Mar Shopping é um centro comercial localizado em Fortaleza, Ceará. Ele possui mais de 200 lojas, incluindo grandes redes de varejo, além de praça de alimentação e cinema. O shopping oferece diversas opções de compras e lazer para os visitantes da capital cearense.
Cell based antiviral assays for screening and profiling of Anti dengue agentsShahan Ullah
1. The document discusses protocols for culturing dengue virus, including culturing the virus in Vero cells and harvesting the viral supernatant.
2. It also describes methods for evaluating potential dengue virus inhibitors, including a plaque reduction assay using BHK-21 cells and a high-throughput antiviral screening assay measuring ATP levels in Vero cells.
3. The protocols require cell lines, growth media, dengue virus samples, reference antiviral compounds, and equipment for cell culture and luminescence detection. Standard procedures are provided for each experiment.
Targeting unfolded protein response in neurodegenerationShahan Ullah
Neurodegenerative diseases like Alzheimer's, Parkinson's, and prion diseases are characterized by the misfolding and aggregation of proteins in the brain. This document discusses how the unfolded protein response (UPR) pathway is activated in prion disease as misfolded prion proteins accumulate. The UPR pathway involves three branches - PERK, IRE1, and ATF6 - that work to reduce protein synthesis and promote protein folding. In prion disease, persistent phosphorylation of the PERK target eIF2α leads to repression of global protein translation. Overexpressing the phosphatase GADD34 or knocking down prion proteins in mouse models prevented eIF2α phosphorylation and neurodegeneration by restoring protein synthesis. In
The document discusses key aspects of Islam including its founder Mahoma and important locations in Mecca such as the Kaaba. Islam was founded by the prophet Mahoma in the 7th century and centers around belief in one God. The most important site in Islam, the Kaaba, is located in Mecca and is the direction of prayer for Muslims around the world.
Recife Antigo is the historic center of Recife, Brazil. It is located along the Capibaribe River and features well-preserved colonial architecture from the 16th-17th centuries including churches, monasteries, and mansions built with coral stone. The area offers a glimpse into Recife's past and is a popular tourist destination known for its culture and architecture.
Olinda and its pre-carnival celebrations are a major tourist attraction in Brazil's northeastern state of Pernambuco. For over a month leading up to carnival, the historic city of Olinda holds daily street parties and concerts to get revelers in the mood for the big event. These festivities showcase Olinda's rich cultural heritage through music, dance, costumes and colorful parades.
O Rio Mar Shopping é um centro comercial localizado em Fortaleza, Ceará. Ele possui mais de 200 lojas, incluindo grandes redes de varejo, além de praça de alimentação e cinema. O shopping oferece diversas opções de compras e lazer para os visitantes da capital cearense.
Cell based antiviral assays for screening and profiling of Anti dengue agentsShahan Ullah
1. The document discusses protocols for culturing dengue virus, including culturing the virus in Vero cells and harvesting the viral supernatant.
2. It also describes methods for evaluating potential dengue virus inhibitors, including a plaque reduction assay using BHK-21 cells and a high-throughput antiviral screening assay measuring ATP levels in Vero cells.
3. The protocols require cell lines, growth media, dengue virus samples, reference antiviral compounds, and equipment for cell culture and luminescence detection. Standard procedures are provided for each experiment.
Targeting unfolded protein response in neurodegenerationShahan Ullah
Neurodegenerative diseases like Alzheimer's, Parkinson's, and prion diseases are characterized by the misfolding and aggregation of proteins in the brain. This document discusses how the unfolded protein response (UPR) pathway is activated in prion disease as misfolded prion proteins accumulate. The UPR pathway involves three branches - PERK, IRE1, and ATF6 - that work to reduce protein synthesis and promote protein folding. In prion disease, persistent phosphorylation of the PERK target eIF2α leads to repression of global protein translation. Overexpressing the phosphatase GADD34 or knocking down prion proteins in mouse models prevented eIF2α phosphorylation and neurodegeneration by restoring protein synthesis. In