Smarten up your Corporate Social Responsibility!
If you and your colleagues would like dry cleaning collected and returned to your office, Cleaners for Causes will happily do this for you and donate 15% of the cost to Butterwick Hospice.
Everyone Wins!
Smarten up your Corporate Social Responsibility!
If you and your colleagues would like dry cleaning collected and returned to your office, Cleaners for Causes will happily do this for you and donate 15% of the cost to Butterwick Hospice.
Everyone Wins!
trabajo final del nivel I de seminario 3
tema : resiliencia - arte
como la creatividad , el arte y los distintos lenguajes plasticos van de la mano de lo que se conoce como Resiliencia.
The document outlines locations and a narrative for a short story about a woman in her mid-20s to early 30s who falls asleep and dreams of surreal situations in different locations that each represent parts of her life. These locations include an office, bedroom, water, mansion, and darkness/woods. Each dream scene will symbolize phases, emotions and life experiences for the main character. Feedback on the idea suggested linking her daily events to each dream scene and noted the artistic freedom and ambition of the proposed surreal short story.
Employer eavesdropping on electronic mail transfer is also widespread and currently not banned by federal wiretapping law. The key in this issue is the tradeoff between productivity versus employee comfort and morale.
Tuned mass damper (TMD) adalah alat kontrol yang terdiri dari massa, pegas, dan peredam yang dipasang pada struktur utama untuk mengurangi getaran akibat beban angin atau gempa. TMD dipasang pada berbagai struktur seperti gedung tinggi, menara, jembatan, dan lainnya. Prinsipnya adalah dengan mengatur frekuensi getaran TMD agar sama dengan frekuensi getaran struktur utama sehingga dapat mengur
O documento parece ser um texto codificado ou criptografado, contendo letras e símbolos aleatórios sem significado aparente. Não é possível entender o conteúdo ou propósito do texto a partir das informações dadas.
Detecting and Quantifying Low Level Variants in Sanger Sequencing TracesThermo Fisher Scientific
Automated fluorescent dye-terminator DNA Sequencing using capillary electrophoresis (also known as CE or Sanger sequencing) has been instrumental in the detailed characterization of the human genome and is now widely used as gold standard method for verification of mutation findings, notably in tumor samples. The primary information of the DNA sequencing process is the identification of the nucleotides and of possible sequence variants. A largely unexplored feature of fluorescent Sanger sequencing traces is the quantitative information embedded therein. With the growing need for quantifying somatic mutations in tumor tissue it is desirable to exploit the potential of the quantitative information obtained from sequencing traces.
Materials and Methods
To this end, we have developed a software tool that converts a Sanger sequencing trace file into a .comma separated value (.csv) file containing numerical data of peak data characteristics that can be explored and analyzed using conventional spreadsheet software. The web-based tool can be accessed at: http://apps.lifetechnologies.com/ab1peakreporter .
The output file contains the peak height and quality values for each nucleotide and peak height ratios for all 4 bases at any given locus allowing the detection and assessment of subtle changes at any given allele.
Results and Discussion
We demonstrate the utility of this tool by analyzing mixed DNA samples with known amounts of spiked in variant alleles from the human TP53 gene ranging from 2.5%, 5%, 7.5%, 10%, 15% and 25% and show that the minor alleles could be readily detected below the 10% level.
Conclusion
Enabling high sensitivity detection of minor alleles with a widely available and simple to use technology like Sanger sequencing will be useful for verification of results obtained from next generation sequencing (NGS) platforms.
trabajo final del nivel I de seminario 3
tema : resiliencia - arte
como la creatividad , el arte y los distintos lenguajes plasticos van de la mano de lo que se conoce como Resiliencia.
The document outlines locations and a narrative for a short story about a woman in her mid-20s to early 30s who falls asleep and dreams of surreal situations in different locations that each represent parts of her life. These locations include an office, bedroom, water, mansion, and darkness/woods. Each dream scene will symbolize phases, emotions and life experiences for the main character. Feedback on the idea suggested linking her daily events to each dream scene and noted the artistic freedom and ambition of the proposed surreal short story.
Employer eavesdropping on electronic mail transfer is also widespread and currently not banned by federal wiretapping law. The key in this issue is the tradeoff between productivity versus employee comfort and morale.
Tuned mass damper (TMD) adalah alat kontrol yang terdiri dari massa, pegas, dan peredam yang dipasang pada struktur utama untuk mengurangi getaran akibat beban angin atau gempa. TMD dipasang pada berbagai struktur seperti gedung tinggi, menara, jembatan, dan lainnya. Prinsipnya adalah dengan mengatur frekuensi getaran TMD agar sama dengan frekuensi getaran struktur utama sehingga dapat mengur
O documento parece ser um texto codificado ou criptografado, contendo letras e símbolos aleatórios sem significado aparente. Não é possível entender o conteúdo ou propósito do texto a partir das informações dadas.
Detecting and Quantifying Low Level Variants in Sanger Sequencing TracesThermo Fisher Scientific
Automated fluorescent dye-terminator DNA Sequencing using capillary electrophoresis (also known as CE or Sanger sequencing) has been instrumental in the detailed characterization of the human genome and is now widely used as gold standard method for verification of mutation findings, notably in tumor samples. The primary information of the DNA sequencing process is the identification of the nucleotides and of possible sequence variants. A largely unexplored feature of fluorescent Sanger sequencing traces is the quantitative information embedded therein. With the growing need for quantifying somatic mutations in tumor tissue it is desirable to exploit the potential of the quantitative information obtained from sequencing traces.
Materials and Methods
To this end, we have developed a software tool that converts a Sanger sequencing trace file into a .comma separated value (.csv) file containing numerical data of peak data characteristics that can be explored and analyzed using conventional spreadsheet software. The web-based tool can be accessed at: http://apps.lifetechnologies.com/ab1peakreporter .
The output file contains the peak height and quality values for each nucleotide and peak height ratios for all 4 bases at any given locus allowing the detection and assessment of subtle changes at any given allele.
Results and Discussion
We demonstrate the utility of this tool by analyzing mixed DNA samples with known amounts of spiked in variant alleles from the human TP53 gene ranging from 2.5%, 5%, 7.5%, 10%, 15% and 25% and show that the minor alleles could be readily detected below the 10% level.
Conclusion
Enabling high sensitivity detection of minor alleles with a widely available and simple to use technology like Sanger sequencing will be useful for verification of results obtained from next generation sequencing (NGS) platforms.
Terrassa is a city located in central Catalonia, Spain. Archaeological evidence indicates the area has been inhabited since prehistoric times. Terrassa originated as the Roman town of Egara, founded in the 1st century BCE. During the medieval period, Terrassa contained an episcopal see and cathedral. In the 19th century, Terrassa played an important role in the industrial revolution specializing in textiles. Today, Terrassa contains several notable Modernista buildings from this era, and is a partner in the Art Nouveau network due to its architectural heritage.
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