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Editor's Notes
Fig. (3). The hypoxic gene profile versus the metastatic gene profile. On the left, the hypoxic gene profile is illustrated. The oxy- gen gradient is indicated from red (normal oxygen levels) to blue (hypoxia). Cells that lie in the periphery of the blood supply become hypoxic and start expressing genes involved in metastasis as indicated by different colors. On the right, a metastatic primary tumor is depicted, containing cells that express genes involved in each sequential step in metastasis formation, thereby constituting the metastatic gene profile. More blood vessels are present here because of the stimulatory effects of hypoxia on angiogenesis. Cells expressing gene products functionally involved in the different steps of metastasis formation are depicted in concordance with these function; i.e. angiogenesis, cell detachment, invasion and seeding. The hypoxic gene profile might de- velop into metastatic profile, and therefore provide a predictive value in clinical decision-making
Fig. (1). Peri-necrotic staining of HIF-1. A) haematoxilin- eosin staining and B) immunohistochemical staining for HIF- 1 in endometriod endometrial carcinoma. Note the nuclear peri-necrotic staining of HIF-1protein (black arrows). Ne- crotic area indicated by N. Magnification 40 x.
Fig. (2). Hypoxic regulation of genes involved in metastasis. Genes are divided by functional implication in metastasis. Furthermore, up or down regulation under hypoxia and in- volvement of HIF-1 or -2 is indicated.
