This document provides information on water volumes and formaldehyde (formalin) treatment dosages for different tanks at a hatchery. It includes a table listing tank names and volumes in liters per 1cm of water depth. Below is a formaldehyde treatment protocol outlining safety procedures and dosage calculations. The protocol instructs the user to calculate the exact dosage needed based on the desired concentration in parts per million (ppm), water depth of the tank in cm, and corresponding formaldehyde dosage in ml per cm. A formaldehyde dosage guide at the bottom provides common treatment concentrations and corresponding formaldehyde amounts in ml per cm.

1. Vol (l) / 1cm
Water Depth 150 175 200 250
Hatchery Weaning R1-R16 45.2 6.8 7.9 9.0 11.3
W1-W8 25.15 3.8 4.4 5.0 6.3
W9-W14 40 6.0 7.0 8.0 10.0
Small Square 10 1.5 1.8 2.0 2.5
R6 25.43 3.8 4.5 5.1 6.4
Nursery N1-N13 61.54 9.2 10.8 12.3 15.4
Raceway 1-3 40 6.0 7.0 8.0 10.0
New Nursery All tanks 70.65 10.6 12.4 14.1 17.7
First Feeding F1-F3 61.54 9.2 10.8 12.3 15.4
Self-cleaning Tanks 49.06 7.4 8.6 9.8 12.3
F9-13 31.4 4.7 5.5 6.3 7.9
Seasite Top Raceways 204.18 30.6 35.7 40.8 51.0
BP's (trial tanks) 196.25 29.4 34.3 39.3 49.1
SP 1,2,11,12 1130 169.5 197.8 226.0 282.5
SP 5,6,7,8,9,10 70.65 10.6 12.4 14.1 17.7
CP's 204.18 30.6 35.7 40.8 51.0
Wrasse Broodstock 70.65 10.6 12.4 14.1 17.7
Simple Formalin Protocol
Ensure fish have been starved
Calculate exact dosages
Switch on O2 diffusers for tanks to be treated
Empty tanks to desired depth for treatment (Use ruler to average depth)
Mix formalin in 10l bucket and introduce to tank (Ask Supervisor about Health and Safety)
Record treatment start time (set alarm for end point)
Regularly check O2 in all tanks throughout
Brush walls and floor inside tanks (deep clean only)
Record treatment on tank sheets (F250/1h = Formalin 250ppm for 1 Hour)
To quickly end treatment lift standpipe and open the inlet to flush out chemical
Switch off O2 when fish have recovered
Check O2 for treated tanks once system has stabilised (open inlet more if low)
Formalin is a CAT 1B Carcinogen therefore you must be trained and competent to use it
Use correct PPE (personal protection equipment) for the task e.g. gloves, gas masks, eye wear.
Formalin Dosage Guide
Do not carry out any treatments if you haven't fully read the COSSH documents for the chemical (can be found in lab)
General Health & Saftey
Desired Concentration mg/l (PPM) Formalin ml/cm
Tanks
Select tank + Concentration, times by water depth during treatment
in cm. e.g. W10 at
250ppm is 10ml/cm, if the water depth is 30cm (10ml*30 = 300ml)
therefore 300ml of formalin is required for the treatment.

2. Inform your supervisor when you go to conduct a treatment encase of emergency
If any direct contact with skin or eyes rinse immediately, contact medical care immediately (eye-wash stations available,
emergency contacts and health and safety representives in office)

3. 350
15.8
8.8
14.0
3.5
8.9
21.5
14.0
24.7
21.5
17.2
11.0
71.5
68.7
395.5
24.7
71.5
24.7
Dosage Guide
COSSH documents for the chemical (can be found in lab)
Desired Concentration mg/l (PPM) Formalin ml/cm

4. act medical care immediately (eye-wash stations available,
fice)