MICROARRAY

Ad

Ad

Ad

Ad

Ad

Ad

Ad

Ad

Ad

Ad

Ad

Ad

1. INTRODUCTION
2. HISTORY
3. PRINCIPLE
4. DNA MICROARRAY TECHNOLOGY
5. PRINCIPLES OF DNA MICROARRAY
TECHNOLOGY
6. TYPES O...

The

large-scale genome sequencing effort and the
ability to immobilize thousands of DNA fragments on
coated glass slide ...



An array is an orderly
arrangement of samples where
matching of known and
unknown DNA samples is done
based on base pai...







Microarray technology evolved from Southern
blotting.
The concept of microarrays was first proposed in the
late 1...



The core principle behind
microarrays is hybridization
between two DNA strands.



Fluorescent labeled target
sequence...







DNA microarray technology may be defined as a
high-throughput and versatile technology used for
parallel gene exp...



Each identified sequenced gene on the glass, silicon
chips or nylon membrane corresponds to a fragment
of genomic DNA, ...





The principle of DNA microarray technology is based
on the fact that complementary sequences of DNA
can be used to h...



Isolate a total RNA containing
mRNA that ideally represents a
quantitative copy of genes
expressed at the time of sampl...






Here, the labelled cDNA
(Sample and Control) are
mixed together.
Purification
After
purification,
the
mixed labell...









Slide is dried and scanned to
determine how much labelled
cDNA (probe) is bound to
each target spot.
Hybridize...

1)

Glass cDNA microarrays which involves the micro
spotting of pre-fabricated cDNA fragments on a glass
slide.

2)

High-...







Glass cDNA microarrays
was the first type of DNA
microarray
technology
developed.
It was pioneered by Patrick
Bro...

Selection of the material to
spot onto the microscope glass
surface.

Preparation and purification of
DNA sequences repres...







Advantages of Glass cDNA microarrays include their
relative affordability with a lower cost.
Its accessibility re...





Glass cDNA microarray have a few disadvantages such
as intensive labour requirement for synthesizing,
purifying, and...






Oligonucleotides are synthesized on the chip.
Presently, the commercial versions of Affymetrix Gene
Chips hold up ...









Light is directed through a
photolithographic
mask
to
specific areas of array surface.
Activation of areas for...



Advantages
offered
by
the
in
situ oligonucleotide array format include
speed, specificity and reproducibility.



In situ oligonucleotide array formats tend to have
expensive specialised equipments e.g. to carry out the
hybridisation...

MICROARRA
Y AS A GENE
EXPRESSION
PROFILING
TOOL

MICROARRA
Y AS A
COMPARATIV
E GENOMICS
TOOL

DISEASE
DIAGNOSIS

DRUG
DISC...



The principle aim of using microarray technology as a gene
expression profiling tool is to answer some of the fundament...



Microarray technology have widespread use in
comparative gene mutation analysis to analyse
genomic alterations such as ...



Different types of cancer have been classified on the
basis of the organs in which the tumors develop.



Now, with th...



Microarray technology has extensive application in
Pharmacogenomics.



Comparative analysis of the genes from a disea...







Microarray technology provides a robust platform for the
research of the impact of toxins on the cells and their ...



Microarray is a recently developed functional genomics
technology that has powerful applications in a wide
array of bio...

THANK YOU

Share Slideshare

LinkedIn
Facebook
Twitter

Embed

Size (px)

Show related Slideshows at end

WordPress Shortcode

Link

Share
Email

Dna microarray (dna chips) by Rachana Tiwari 149083 views
Microarray by ruchibioinfo 24019 views
Microarray by microbiologiist 16806 views
Tilling and Ecotilling for crop imp... by Devidas Thombare 6923 views
Use of Affymetrix Arrays (GeneChip®... by Affymetrix 2580 views
Drug Resistance Mechanism by Santosh Kumar Yadav 91140 views

View on Slideshare

1 of 30 Ad

View on Slideshare

1 of 30 Ad