SlideShare a Scribd company logo
We are Growing!




    “IntelliBond Trace Minerals”
    Shattering Trace Mineral Perceptions!

1
Micronutrients

    • Second largest manufacturer and supplier of trace mineral
      nutrition in NA
    • Dedicated only to trace mineral nutrition in livestock
    • Third party auditors certify high product quality
       – HAACP, ISO 9002, Safe Feed, Safe Food, FAMIQs
    • All products American made in Indianapolis, In




2
IntelliBond Trace Minerals:

    • Fully defined crystalline structures
       – High trace metal potency
       – Non-hygroscopic
       – Not soluble above pH 4.0
    • Complete rumen by-pass
    • Increased trace metal absorption
    • Greater stability of essential nutrients (vitamins, etc.)




3
Trace Mineral Nutrition in Ruminants

    • Sulfates / Oxides used from the 50’s
       – Highly reactive in feeds and the rumen
       – Lower cost per unit of metal
    • Organics developed in the 1970’s and 80’s
       – Improved stability in feeds
       – Better trace mineral absorption
       – High cost per unit of metal
    • Hydroxy trace minerals (IntelliBond®)
       –   No adverse impact on essential nutrients in feeds
       –   Complete rumen bypass of essential metals
       –   Increased trace metal absorption in the small intestine
       –   Reduced cost per unit of metal
4
Smart Release Crystals -
Protect Essential Nutrients Present in Premixes & Feeds

                         Mash, Broiler diet with 3.6% added fat

                         70                                  Control (18 ppm)
                                                             Cu sulfate (200 ppm)
Feed Vitamin E (mg/kg)




                         60                                  IntelliBond C (200 ppm)

                         50

                         40
                                            *

                         30                             *                                  Feed levels correspond to lower
                                                                                           liver and plasma Vit. E in CuSO4
                         20          32% loss in
                                                                      *                *          fed chicks (p<0.05).
                                  Vitamin E activity
                         10          at 10 days

                          0
                              0            10          20           30              40
                                                Storage time (d)
                 * Within a time point, mean is significantly different from Control (p<0.05).
    5
                                                                           Data adapted from Lu et al., 2010. Biol. Trace Elem. Res. 138:181-9.
Impact of Vitamin E Degradation
    Assumptions:
    • Cow receiving 750 IU Vit. E per day in a 3.0 lb supplement, that also delivers -
        • 15 ppm copper 75 ppm Zinc and 45 ppm manganese in final ration
    • Supplement specs = 0.055% Vit E, 243 ppm Cu, 1,213 ppm Zn & 728 ppm Mn
    • Premix sustains a 20% loss in its Vit. E content within two week storage period


    Results:
    • At 750 IUs per day, cow consumes .55 Kgs per year, E 50% ($9.30 Cow/yr)
        •   750 IUs / 50% = 1,500 mg/d E 50% X 365 days = .548 Kgs / yr x $17.00 Kg)
    • 20% loss = .11 Kg Vit. E 50% resulting in a loss of $1.86 / cow in Vit. E activity)

    Pay back: (Greater than 4.0:1)
    • Cost of 15 ppm Cu from CuSO4 = .48 Kgs ($1.35) cow per year
    • Cost of 15 ppm from IntelliBond C = .21 Kgs ($1.80) cow per year
6
    • Incremental cost to replace copper sulfate is $.45 cow per year
Smart Release Crystals -
Support Rumen / Abomasum By-Pass

        O          O
              S
         O        O

        X
H2O               OH2
         Cu                                             Typical Organic Trace Mineral

H2O               OH2
Inorganic trace mineral
                             IntelliBond C                                      Cl
                          Defined Crystal Matrix   OH Stretched Covalent Bond

                                             HO         OH              HO           OH
                                                   Cu                           Cu
                                             HO         OH              HO           OH
                            At a pH                          Covalent Bond
                            of < 3.5
7                                                  Cl     Soluble form          Cl
Smart Release Crystals –
Increase Trace Mineral Intestinal Absorption
                                                                    IntelliBond C Relative Bioavailability In Cattle
                                         Trial Design:
                                                                       Dependent Variable    Copper           RBV           P=
                                         •98 day depletion period                            source
                                         •60 steers
                                         •3 X 2 factorial setup           Plasma Cu           CuSO4            100         0.07
                                         •10 steers / treatment                                 IBc            132
                                         •Trts @ 0, 5 & 10 ppm Cu
                                                                          Liver Cu            CuSO4            100         0.04
                                         •5 ppm Mo & .15 % S
                                                                                                IBc            196
                                                                                             J.W. Spears et al. N.C. State University


Trial Design:
•16 yearling steers                            IntelliBond Z Relative Bioavailability In Cattle
•Depleted 14 days                                                                                                    2X
                                               Dependent   Variable           Zinc     RBV          P=
     (basal @ 25 ppm Zn)                                                     Source                               Increase
•Treatments:                                                                                                       in RBV
      Zinc sulfate @ 25 ppm                      Absorbed Zn                 ZnSO4     100          0.01
      IntelliBond Z @ 25 ppm
                                                                               IBz     205
•Seven day adaptation
•Five day total collection
                                                 Retained Zn                 ZnSO4     100          0.01
     Urine & fecal output
                                                                               IBz     204
     Feed in & Orts
    J.W. Spears et al. N.C. State University
IntelliBond C vs. OTM In Broilers:
• Ross birds grown to 56 days of age
• Floor pens, w/ used litter (.8 ft2 / bird)
• 10 replicates of each treatment (25 birds per pen)       Results:
                             Added Copper Levels (ppm)
                                                           •All sources improved F/G
     Test Materials        Starter    Grower    Finisher   •No significant difference at any point
 Control (no added Cu)      None        None     None
         TBCC                 25         25       25
         TBCC                 50         50       50
         TBCC                 75         75       75
         TBCC                100         100      100
         TBCC                125         125      125
         TBCC                150         150      150
         TBCC                175         175      175
  Cu Metal Proteinate         25         25       25
  Cu Metal Proteinate         50         50       50
  Cu Metal Proteinate         75         75       75
  Cu Metal Proteinate        100         100      100
Cu Amino Acid Complex         25         25       25
Cu Amino Acid Complex         50         50       50
Cu Amino Acid Complex         75         75       75
Cu Amino Acid Complex        100         100      100                            A.H. Pharma Research Institute
IntelliBond C vs. Cu Lysine in Lambs

Study Design:                                                                    •   Treatments:
•50 wether lambs w/ initial BW of 23.8 Kg                                             – Control: – no supplemental copper
•10 lambs / trt, individually housed in wood pens                                     – Trt 2: 10 ppm Cu from Cu Lysine
•All lambs on common diet first 3 weeks                                               – Trt 3: 20 ppm Cu from Cu Lysine
•At week 4, lambs weighed & blocked into 5 trts                                       – Trt 4: 10 ppm Cu from IntelliBond C
•60 day evaluation                                                                    – Trt 5: 20 ppm Cu from IntelliBond C
•     Orts were recorded and feed intake adj’d weekly                            •   Measurements:
                                                                                      – Blood drawn at days 0, 30 and 60
                                                                                      – All lambs harvested at 60 days, livers
IntelliBond C and Cu-lysine were of similar                                               collected and assayed
availability when fed to finishing lambs.
                                                            Treatments                                      Contrast P Value


                                                     Cu          Cu                  IB C           Cont    10 vs       IB C     IB C
                                                   Lysine      Lysine     IB C        20             Vs       20       vs Lys   vs Lys
  Item                              Control        10 ppm      20 ppm    10 ppm       ppm    SEM     Cu      ppm         10       20
  Plasma Cu (mg/l)

      Day 0                           0.74           0.84       0.78      0.77       0.81    0.02   0.29        0.82   0.35     0.67

      Day 60                          0.89           0.96       1.08      1.03       1.09    0.03   0.02        0.11   0.32     0.89

  Liver Cu (mg/Kg)                    45.4          278.5       373.8    297.0       405.1   29.8   0.01        0.01   0.47     0.23

 2011. Biol. Trace Elements Res. Vol 144. pp 570 - 579.                                                    10
IntelliBond C vs. Availa Cu in Yearlings

Study Design:                                            IntelliBond C and Availa Cu were
•24 Brahman crossbred steers                             of similar availability when fed to
•72 day study                                                      yearling steers
•4 Trt / 6 pens per
•Trt’s                                                     All cattle on 10 ppm Cu Availa Cu
     –   10 ppm Cu – Availa Cu
     –   10 ppm Cu – IntelliBond C
     –   15 ppm Cu Availa Cu + 15 ppm Cu IntelliBond C
     –   30 ppm Cu IntelliBond C                                                                  IBC/
•Free-choice access to star-grass hay                              IBC   Availa C IBC            Availa C
•Fed molasses supplement with Trts                        Day     10 ppm 10 ppm 30 ppm           30 ppm
•Measurements – Liver copper at:
                                                           0       146       161      125          158
     –   0, 24, 48 & 72 days
                                                           24      136       151      182          163
                                                           48      143       145      195          151
                                                           72      166       157      226          213

                                                         *Liver Cu expressed as PPM Dry Matter
 J. Anim. Sci. 2007 85:871-876
 J. Arthington, Ph.D / J. Spears, Ph.D.
IntelliBond® Value Proposition
Ruminants

                                                                              Mineral Cost1
                                                                               (Cow / Year)
        100% Sulfates    2
                             (many known antagonists)

                                                                                 $5.16

        100% Organic Minerals 2 (OTM)
                                                                                 $37.40

        75% Sulfates 2                                       +    25% OTM 2
                                                                                 $13.22
    =   100% Hydroxy Trace Minerals 2 (IntelliBond®)
                                                                                 $11.77
=
        80% Hydroxy Trace Minerals
                                                                                  $9.42

        1) Mineral cost for a lactating dairy cow consuming 22 Kgs DM / day
        2) Supplementation levels
              1) Zinc @ 80 ppm
              2) Manganese @ 40 ppm
              3) Copper @15 ppm
Cornell – Transition Cow Study Design

     Design:
     •   Transition cow study (21 days prepartum through 84 days post partum)
     •   60 total cows – 3 treatments of 20 cows each
          – Cows individually fed (tie stall)
     •   Cows milked 2x / day, within the Cornell milking facility
     •   Milk weighed at each milking
     •   Separate composite milk sample taken from AM / PM milkings weekly

     Treatments:       (zinc @ 60 ppm; Mn @ 40 ppm & Cu @ 15 ppm)
          – All sulfate
          – 75% sulfate / 25% Availa Zn, Mn & Cu
          – All IntelliBond (hydroxy trace minerals)


     Measurements:
     •   All performance parameters
13   •   Cow well being & health                            T. Overton, Ph.D, Cornell University, Ithaca, NY
Cornell Transition: Fat Corrected Milk




14
Cornell Transition: Performance Results

        Parameter        Inorganic     ITM / Availa      IntelliBond   SEM    Trt.         Week        Trt. / Week

      DMI (Kg/d)
      Prepartum             15.0           14.8             14.8       .03    NS           <.0001           NS
      Postpartum            25.4           24.5             25.8       .05    NS           <.0001           NS

      Milk (Kg/d)           45.3           45.7             46.6       1.1    NS           <.0001          0.019

      Fat (Kg/d)            1.48           1.45             1.49       .05    NS           <.0001          0.109

      Protein (Kg/d)        1.34           1.33             1.37       .04    NS           0.002            NS

      Lactose (Kg/d)        2.14           2.16             2.18       .05    NS           <.0001          0.053

      FCM (Kg/d)            43.6           43.3             44.3       1.15   NS           <.0001          0.027




  No statistical differences in Gait Scores, SCC, BCS or other health parameters




 15
             Availa® - Registered Trademark of Zinpro Corporation             T. Overton, Ph.D, Cornell University, Ithaca, NY
Cornell Transition: Oxidative Stress

                                                          Treatments                                             P-value

 Item                                        ITM          ITM/OTM            IntelliBond SEM              Trt      Wk      Trt x Wk

 TBARS, uM              (Thiobarbituric acid-reactive substances)


 Whole study                               2.11A            1.98AB                1.95B            0.07   0.16    <.01      0.40

 Prepartum                                 1.47              1.49                 1.38             0.06   0.37    0.16      0.74

 Postpartum                                2.26              2.11                 2.10             0.09   0.29    <.01      0.23


 TAC, mM             (Total antioxidant capacity)

 Whole study                               2.14a            2.07ab                1.93b            0.07   0.09    <.01       0.94

 Prepartum                                 2.09a            1.93ab                1.84b            0.08   0.10    0.01      0.86

 Postpartum                                2.16a            2.09ab                1.95b            0.08   0.12    <.01      0.88
     A,B
           Least square means within row with different upper-case superscript tend to differ (P < 0.1)
16
     a,b
           Least square means within row with different lower-case superscript differ (P < 0.05)
Projected Economics:
  Parameter              Inorganic                 Combo                 IntelliBond      IntelliBond Incremental Value
                        Lbs (value)           (ITM/OTM)                  Lbs (value)             ITM vs. IB     Combo
                                                  Lbs (value)                                          vs. IB

Milk (lbs)            99.9     ($19.17)        100.8 ($19.34)           102.7 ($19.71)     $.54 cow/d     $.37 cow/d

Fat (lbs)              3.26     ($5.68)         3.20      ($5.57)       3.28   ($5.71)     $.03 cow/d     $.14 cow/d

Protein (lbs)          2.95     ($9.78)         2.93      ($9.71)       3.02   ($10.00)    $.22 cow/d     $.29 cow/d

Lactose (lbs)         4.72       ($2.21)        4.76          ($2.23)   4.81   ($2.25)     $.04 cow/d     $.02 cow/d

Program Cost           $.0130 cow/d              $.0325 cow/d           $.0295 cow/d      $.0165 cow/d -$.003 cow/d


Milk pricing based on N.E. Milk Order as of Dec. 31st, 2011




 17
Key Value Points:
•    Fully defined crystalline structure
•    Increased metal potency
•    Maintains essential nutrient potency in the supplement / feed
•    Rumen By-Pass
      – Increased rumen function (DM digestibility)
• Crystalline structure provides for sustained release and improved
  absorption of the mineral in the intestinal tract
• Increased relative bioavailability (equivalent or better than best OTMs)
      – Greater essential mineral absorption drives cow productivity and health
      – Ability to reduce total mineral fed, reducing cost & environmental compliance


     The only cost effective means to completely replace Trace
               Mineral sulfate / oxides forms in the diet
18
Thank You!
19

More Related Content

What's hot

Trash to Treasure: Waste-to-energy as next fuel source?
Trash to Treasure:Waste-to-energy as next fuel source?Trash to Treasure:Waste-to-energy as next fuel source?
Trash to Treasure: Waste-to-energy as next fuel source?
French American Chamber of Commerce - San Francisco
 
Our featured product - PolyCarb
Our featured product - PolyCarbOur featured product - PolyCarb
Our featured product - PolyCarb
Satyajit Chemicals Pvt Ltd
 
New LC-IR Technique To Characterize Polymeric Excipients In Pharmaceutical Fo...
New LC-IR Technique To Characterize Polymeric Excipients In Pharmaceutical Fo...New LC-IR Technique To Characterize Polymeric Excipients In Pharmaceutical Fo...
New LC-IR Technique To Characterize Polymeric Excipients In Pharmaceutical Fo...
mzhou45
 
Screening Assays For Gpc Rs 3
Screening Assays For Gpc Rs 3Screening Assays For Gpc Rs 3
Screening Assays For Gpc Rs 3
Shirley Pullan
 
Oct 2010 sci pres
Oct 2010 sci presOct 2010 sci pres
Oct 2010 sci pres
intermedx
 
Ppt tp(2)
Ppt tp(2)Ppt tp(2)
Ppt tp(2)
guestee784c
 
17 leco's citius high res lcms-faster results-fewer runs david schlegel - lec...
17 leco's citius high res lcms-faster results-fewer runs david schlegel - lec...17 leco's citius high res lcms-faster results-fewer runs david schlegel - lec...
17 leco's citius high res lcms-faster results-fewer runs david schlegel - lec...
CPSA-2012_5-Minutes-Fame
 
Cropworld poster 2012
Cropworld poster 2012Cropworld poster 2012
Cropworld poster 2012
JJDempsey
 

What's hot (8)

Trash to Treasure: Waste-to-energy as next fuel source?
Trash to Treasure:Waste-to-energy as next fuel source?Trash to Treasure:Waste-to-energy as next fuel source?
Trash to Treasure: Waste-to-energy as next fuel source?
 
Our featured product - PolyCarb
Our featured product - PolyCarbOur featured product - PolyCarb
Our featured product - PolyCarb
 
New LC-IR Technique To Characterize Polymeric Excipients In Pharmaceutical Fo...
New LC-IR Technique To Characterize Polymeric Excipients In Pharmaceutical Fo...New LC-IR Technique To Characterize Polymeric Excipients In Pharmaceutical Fo...
New LC-IR Technique To Characterize Polymeric Excipients In Pharmaceutical Fo...
 
Screening Assays For Gpc Rs 3
Screening Assays For Gpc Rs 3Screening Assays For Gpc Rs 3
Screening Assays For Gpc Rs 3
 
Oct 2010 sci pres
Oct 2010 sci presOct 2010 sci pres
Oct 2010 sci pres
 
Ppt tp(2)
Ppt tp(2)Ppt tp(2)
Ppt tp(2)
 
17 leco's citius high res lcms-faster results-fewer runs david schlegel - lec...
17 leco's citius high res lcms-faster results-fewer runs david schlegel - lec...17 leco's citius high res lcms-faster results-fewer runs david schlegel - lec...
17 leco's citius high res lcms-faster results-fewer runs david schlegel - lec...
 
Cropworld poster 2012
Cropworld poster 2012Cropworld poster 2012
Cropworld poster 2012
 

Viewers also liked

Trace minerals
Trace mineralsTrace minerals
Trace minerals
obanbrahma
 
Major minerals
Major mineralsMajor minerals
Major minerals
obanbrahma
 
Mineral..
Mineral..Mineral..
Mineral..
hanibhk5
 
trace minerals, Zn, Co
trace minerals, Zn, Cotrace minerals, Zn, Co
trace minerals, Zn, Co
Dr. Latika Yadav
 
Minerals
MineralsMinerals
Minerals
dr esha bali
 
Minerals
MineralsMinerals
Minerals
Abigail Abalos
 
Mineral Metabolism
Mineral MetabolismMineral Metabolism
Mineral Metabolism
Tapeshwar Yadav
 
Essential trace elements
Essential trace elementsEssential trace elements
Essential trace elements
Dr Manju prasad
 
Minerals slideshare
Minerals slideshareMinerals slideshare
Minerals slideshare
cmerkert
 
Fn1 ppt. vitamins and minerals
Fn1 ppt. vitamins and mineralsFn1 ppt. vitamins and minerals
Fn1 ppt. vitamins and minerals
MsKPetty
 

Viewers also liked (10)

Trace minerals
Trace mineralsTrace minerals
Trace minerals
 
Major minerals
Major mineralsMajor minerals
Major minerals
 
Mineral..
Mineral..Mineral..
Mineral..
 
trace minerals, Zn, Co
trace minerals, Zn, Cotrace minerals, Zn, Co
trace minerals, Zn, Co
 
Minerals
MineralsMinerals
Minerals
 
Minerals
MineralsMinerals
Minerals
 
Mineral Metabolism
Mineral MetabolismMineral Metabolism
Mineral Metabolism
 
Essential trace elements
Essential trace elementsEssential trace elements
Essential trace elements
 
Minerals slideshare
Minerals slideshareMinerals slideshare
Minerals slideshare
 
Fn1 ppt. vitamins and minerals
Fn1 ppt. vitamins and mineralsFn1 ppt. vitamins and minerals
Fn1 ppt. vitamins and minerals
 

Crystal dairy express 0113

  • 1. We are Growing! “IntelliBond Trace Minerals” Shattering Trace Mineral Perceptions! 1
  • 2. Micronutrients • Second largest manufacturer and supplier of trace mineral nutrition in NA • Dedicated only to trace mineral nutrition in livestock • Third party auditors certify high product quality – HAACP, ISO 9002, Safe Feed, Safe Food, FAMIQs • All products American made in Indianapolis, In 2
  • 3. IntelliBond Trace Minerals: • Fully defined crystalline structures – High trace metal potency – Non-hygroscopic – Not soluble above pH 4.0 • Complete rumen by-pass • Increased trace metal absorption • Greater stability of essential nutrients (vitamins, etc.) 3
  • 4. Trace Mineral Nutrition in Ruminants • Sulfates / Oxides used from the 50’s – Highly reactive in feeds and the rumen – Lower cost per unit of metal • Organics developed in the 1970’s and 80’s – Improved stability in feeds – Better trace mineral absorption – High cost per unit of metal • Hydroxy trace minerals (IntelliBond®) – No adverse impact on essential nutrients in feeds – Complete rumen bypass of essential metals – Increased trace metal absorption in the small intestine – Reduced cost per unit of metal 4
  • 5. Smart Release Crystals - Protect Essential Nutrients Present in Premixes & Feeds Mash, Broiler diet with 3.6% added fat 70 Control (18 ppm) Cu sulfate (200 ppm) Feed Vitamin E (mg/kg) 60 IntelliBond C (200 ppm) 50 40 * 30 * Feed levels correspond to lower liver and plasma Vit. E in CuSO4 20 32% loss in * * fed chicks (p<0.05). Vitamin E activity 10 at 10 days 0 0 10 20 30 40 Storage time (d) * Within a time point, mean is significantly different from Control (p<0.05). 5 Data adapted from Lu et al., 2010. Biol. Trace Elem. Res. 138:181-9.
  • 6. Impact of Vitamin E Degradation Assumptions: • Cow receiving 750 IU Vit. E per day in a 3.0 lb supplement, that also delivers - • 15 ppm copper 75 ppm Zinc and 45 ppm manganese in final ration • Supplement specs = 0.055% Vit E, 243 ppm Cu, 1,213 ppm Zn & 728 ppm Mn • Premix sustains a 20% loss in its Vit. E content within two week storage period Results: • At 750 IUs per day, cow consumes .55 Kgs per year, E 50% ($9.30 Cow/yr) • 750 IUs / 50% = 1,500 mg/d E 50% X 365 days = .548 Kgs / yr x $17.00 Kg) • 20% loss = .11 Kg Vit. E 50% resulting in a loss of $1.86 / cow in Vit. E activity) Pay back: (Greater than 4.0:1) • Cost of 15 ppm Cu from CuSO4 = .48 Kgs ($1.35) cow per year • Cost of 15 ppm from IntelliBond C = .21 Kgs ($1.80) cow per year 6 • Incremental cost to replace copper sulfate is $.45 cow per year
  • 7. Smart Release Crystals - Support Rumen / Abomasum By-Pass O O S O O X H2O OH2 Cu Typical Organic Trace Mineral H2O OH2 Inorganic trace mineral IntelliBond C Cl Defined Crystal Matrix OH Stretched Covalent Bond HO OH HO OH Cu Cu HO OH HO OH At a pH Covalent Bond of < 3.5 7 Cl Soluble form Cl
  • 8. Smart Release Crystals – Increase Trace Mineral Intestinal Absorption IntelliBond C Relative Bioavailability In Cattle Trial Design: Dependent Variable Copper RBV P= •98 day depletion period source •60 steers •3 X 2 factorial setup Plasma Cu CuSO4 100 0.07 •10 steers / treatment IBc 132 •Trts @ 0, 5 & 10 ppm Cu Liver Cu CuSO4 100 0.04 •5 ppm Mo & .15 % S IBc 196 J.W. Spears et al. N.C. State University Trial Design: •16 yearling steers IntelliBond Z Relative Bioavailability In Cattle •Depleted 14 days 2X Dependent Variable Zinc RBV P= (basal @ 25 ppm Zn) Source Increase •Treatments: in RBV Zinc sulfate @ 25 ppm Absorbed Zn ZnSO4 100 0.01 IntelliBond Z @ 25 ppm IBz 205 •Seven day adaptation •Five day total collection Retained Zn ZnSO4 100 0.01 Urine & fecal output IBz 204 Feed in & Orts J.W. Spears et al. N.C. State University
  • 9. IntelliBond C vs. OTM In Broilers: • Ross birds grown to 56 days of age • Floor pens, w/ used litter (.8 ft2 / bird) • 10 replicates of each treatment (25 birds per pen) Results: Added Copper Levels (ppm) •All sources improved F/G Test Materials Starter Grower Finisher •No significant difference at any point Control (no added Cu) None None None TBCC 25 25 25 TBCC 50 50 50 TBCC 75 75 75 TBCC 100 100 100 TBCC 125 125 125 TBCC 150 150 150 TBCC 175 175 175 Cu Metal Proteinate 25 25 25 Cu Metal Proteinate 50 50 50 Cu Metal Proteinate 75 75 75 Cu Metal Proteinate 100 100 100 Cu Amino Acid Complex 25 25 25 Cu Amino Acid Complex 50 50 50 Cu Amino Acid Complex 75 75 75 Cu Amino Acid Complex 100 100 100 A.H. Pharma Research Institute
  • 10. IntelliBond C vs. Cu Lysine in Lambs Study Design: • Treatments: •50 wether lambs w/ initial BW of 23.8 Kg – Control: – no supplemental copper •10 lambs / trt, individually housed in wood pens – Trt 2: 10 ppm Cu from Cu Lysine •All lambs on common diet first 3 weeks – Trt 3: 20 ppm Cu from Cu Lysine •At week 4, lambs weighed & blocked into 5 trts – Trt 4: 10 ppm Cu from IntelliBond C •60 day evaluation – Trt 5: 20 ppm Cu from IntelliBond C • Orts were recorded and feed intake adj’d weekly • Measurements: – Blood drawn at days 0, 30 and 60 – All lambs harvested at 60 days, livers IntelliBond C and Cu-lysine were of similar collected and assayed availability when fed to finishing lambs. Treatments Contrast P Value Cu Cu IB C Cont 10 vs IB C IB C Lysine Lysine IB C 20 Vs 20 vs Lys vs Lys Item Control 10 ppm 20 ppm 10 ppm ppm SEM Cu ppm 10 20 Plasma Cu (mg/l) Day 0 0.74 0.84 0.78 0.77 0.81 0.02 0.29 0.82 0.35 0.67 Day 60 0.89 0.96 1.08 1.03 1.09 0.03 0.02 0.11 0.32 0.89 Liver Cu (mg/Kg) 45.4 278.5 373.8 297.0 405.1 29.8 0.01 0.01 0.47 0.23 2011. Biol. Trace Elements Res. Vol 144. pp 570 - 579. 10
  • 11. IntelliBond C vs. Availa Cu in Yearlings Study Design: IntelliBond C and Availa Cu were •24 Brahman crossbred steers of similar availability when fed to •72 day study yearling steers •4 Trt / 6 pens per •Trt’s All cattle on 10 ppm Cu Availa Cu – 10 ppm Cu – Availa Cu – 10 ppm Cu – IntelliBond C – 15 ppm Cu Availa Cu + 15 ppm Cu IntelliBond C – 30 ppm Cu IntelliBond C IBC/ •Free-choice access to star-grass hay IBC Availa C IBC Availa C •Fed molasses supplement with Trts Day 10 ppm 10 ppm 30 ppm 30 ppm •Measurements – Liver copper at: 0 146 161 125 158 – 0, 24, 48 & 72 days 24 136 151 182 163 48 143 145 195 151 72 166 157 226 213 *Liver Cu expressed as PPM Dry Matter J. Anim. Sci. 2007 85:871-876 J. Arthington, Ph.D / J. Spears, Ph.D.
  • 12. IntelliBond® Value Proposition Ruminants Mineral Cost1 (Cow / Year) 100% Sulfates 2 (many known antagonists) $5.16 100% Organic Minerals 2 (OTM) $37.40 75% Sulfates 2 + 25% OTM 2 $13.22 = 100% Hydroxy Trace Minerals 2 (IntelliBond®) $11.77 = 80% Hydroxy Trace Minerals $9.42 1) Mineral cost for a lactating dairy cow consuming 22 Kgs DM / day 2) Supplementation levels 1) Zinc @ 80 ppm 2) Manganese @ 40 ppm 3) Copper @15 ppm
  • 13. Cornell – Transition Cow Study Design Design: • Transition cow study (21 days prepartum through 84 days post partum) • 60 total cows – 3 treatments of 20 cows each – Cows individually fed (tie stall) • Cows milked 2x / day, within the Cornell milking facility • Milk weighed at each milking • Separate composite milk sample taken from AM / PM milkings weekly Treatments: (zinc @ 60 ppm; Mn @ 40 ppm & Cu @ 15 ppm) – All sulfate – 75% sulfate / 25% Availa Zn, Mn & Cu – All IntelliBond (hydroxy trace minerals) Measurements: • All performance parameters 13 • Cow well being & health T. Overton, Ph.D, Cornell University, Ithaca, NY
  • 14. Cornell Transition: Fat Corrected Milk 14
  • 15. Cornell Transition: Performance Results Parameter Inorganic ITM / Availa IntelliBond SEM Trt. Week Trt. / Week DMI (Kg/d) Prepartum 15.0 14.8 14.8 .03 NS <.0001 NS Postpartum 25.4 24.5 25.8 .05 NS <.0001 NS Milk (Kg/d) 45.3 45.7 46.6 1.1 NS <.0001 0.019 Fat (Kg/d) 1.48 1.45 1.49 .05 NS <.0001 0.109 Protein (Kg/d) 1.34 1.33 1.37 .04 NS 0.002 NS Lactose (Kg/d) 2.14 2.16 2.18 .05 NS <.0001 0.053 FCM (Kg/d) 43.6 43.3 44.3 1.15 NS <.0001 0.027 No statistical differences in Gait Scores, SCC, BCS or other health parameters 15 Availa® - Registered Trademark of Zinpro Corporation T. Overton, Ph.D, Cornell University, Ithaca, NY
  • 16. Cornell Transition: Oxidative Stress Treatments P-value Item ITM ITM/OTM IntelliBond SEM Trt Wk Trt x Wk TBARS, uM (Thiobarbituric acid-reactive substances) Whole study 2.11A 1.98AB 1.95B 0.07 0.16 <.01 0.40 Prepartum 1.47 1.49 1.38 0.06 0.37 0.16 0.74 Postpartum 2.26 2.11 2.10 0.09 0.29 <.01 0.23 TAC, mM (Total antioxidant capacity) Whole study 2.14a 2.07ab 1.93b 0.07 0.09 <.01 0.94 Prepartum 2.09a 1.93ab 1.84b 0.08 0.10 0.01 0.86 Postpartum 2.16a 2.09ab 1.95b 0.08 0.12 <.01 0.88 A,B Least square means within row with different upper-case superscript tend to differ (P < 0.1) 16 a,b Least square means within row with different lower-case superscript differ (P < 0.05)
  • 17. Projected Economics: Parameter Inorganic Combo IntelliBond IntelliBond Incremental Value Lbs (value) (ITM/OTM) Lbs (value) ITM vs. IB Combo Lbs (value) vs. IB Milk (lbs) 99.9 ($19.17) 100.8 ($19.34) 102.7 ($19.71) $.54 cow/d $.37 cow/d Fat (lbs) 3.26 ($5.68) 3.20 ($5.57) 3.28 ($5.71) $.03 cow/d $.14 cow/d Protein (lbs) 2.95 ($9.78) 2.93 ($9.71) 3.02 ($10.00) $.22 cow/d $.29 cow/d Lactose (lbs) 4.72 ($2.21) 4.76 ($2.23) 4.81 ($2.25) $.04 cow/d $.02 cow/d Program Cost $.0130 cow/d $.0325 cow/d $.0295 cow/d $.0165 cow/d -$.003 cow/d Milk pricing based on N.E. Milk Order as of Dec. 31st, 2011 17
  • 18. Key Value Points: • Fully defined crystalline structure • Increased metal potency • Maintains essential nutrient potency in the supplement / feed • Rumen By-Pass – Increased rumen function (DM digestibility) • Crystalline structure provides for sustained release and improved absorption of the mineral in the intestinal tract • Increased relative bioavailability (equivalent or better than best OTMs) – Greater essential mineral absorption drives cow productivity and health – Ability to reduce total mineral fed, reducing cost & environmental compliance The only cost effective means to completely replace Trace Mineral sulfate / oxides forms in the diet 18

Editor's Notes

  1. Recently completed plant expansion for the manufacture of IntelliBond Z and M represents a significant investment in Micronutrients’ commitment to the North American dairy business. The plant is located in Indianapolis, IN and is open for your inspection should you travels bring you to Indianapolis. Micronutrients’ IntelliBond C manufacturing plant is located at the same site. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
  2. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt The use of IntelliBond products, especially the replacement of Cu sulfate given its classification as a pro oxidant (Ref: Wikipedia) can significant reduce the destruction of essential nutrients (Vitamins, DFMs, etc.) in feeds and premixes. The above study involved a layer mash feed containing 3.6% added fat that was stored in a bin at approximately 70 degrees F. You will note that the feed containing IntelliBond C did not promote oxidation (present in the Cu Sulfate trt.). In fact results indicated that over 30% of the tocopherol was destroyed in the first 30 days. We find this same type of situation occurring in the dairy industry given the use of various by products within various commodity blends / supplements used to also deliver the vitamins and trace minerals in the same package
  3. As a follow up to the prior slide this slide is designed to highlight the negative economic impact of sulfates on vita E stability in a premix. The slide is designed to quickly show the effect of a 20% loss in Vit. E activity within a supplement being fed at the rate of 1 lbs / hd / day. This can result in a loss of $1.65 per hd/yr. When you calculate the incremental cost of moving from copper sulfate to IntelliBond C of $.40 per cow per year, just the reduction in lost Vit e activity provided a ROI of 4:1. Please note the trace mineral levels highlighted under assumptions assumes a ration that is designed to deliver 15 ppm Cu, 80 ppm Zn and 40 ppm Mn and is delivered to the cow in the 1 lbs supplement. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
  4. All IntelliBond Trace minerals are rumen by pass allowing them to escape interaction with the many antagonists that routinely affect trace minerals stability and bioavailability in the rumen. Given their ability to resist going into solution at a pH above 3.5 they are naturally By-passed to the abomasum with limited negative impact. Each IntelliBond TM form incorporates a covalent bond between its metal and the OH group. Like better quality organic trace minerals the covalent bond structure protects the metal as it makes it way from the rumen to the small intestine. In addition to the functionality of its covalent bond all IntelliBond products exist in a crystalline matrix, which provides an additional level of protection when the crystal is moving through the rumen, protecting it from antagonisms. Once IntelliBond crystals enter the abomasum, they encounter an area of low pH allowing the crystals to move from their crystalline form into solution, still stabilized by the presence of the covalent bond between the metal and the OH groups. Once in solution the IntelliBond metals are now available for absorption, following their transit to the small intestine, allowing for increased bioavailability of the metal to the animal. Crystal References: IntelliBond C - http://en.wikipedia.org/wiki/Atacamite IntelliBond Z - http://en.wikipedia.org/wiki/Simonkolleite IntelliBond M - http://webmineral.com/data/Kempite.shtml . 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
  5. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt The crystal form of each IntelliBond product is slowly solubilized in the abomasum allowing for more efficiently absorption of the metal over the full length of the small intestine improving trace mineral absorption efficacy. The two studies highlighted above indicate the superior bioavailability of IntelliBond products when in a model utilized extensively by several organic trace mineral companies to validate the bio-efficacy of their products. It is interesting that no major organic trace mineral company support their products based on relative bioavailability given the fact that published research demonstrates a potential benefit of 10 to 15%, however their pricing is more in the range of 600 – 700% of the value of typical sulfate forms. IntelliBond trace minerals represent an opportunity to significantly improve trace mineral absorption at a price point that is far less than typical organic trace mineral forms.
  6. The study provides a head to head comparison between IntelliBond C fed at 25 to 100 ppm with two leading organic sources (Availa Copper and BioPlex copper) fed at ISO levels under commercial conditions. You will see that there were no statistically significant differences between the sources at each level of comparsion. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
  7. A study was conducted to evaluate the relative bioavailability of IntelliBond C (tri basic copper chloride) in lambs compared with Copper Lysine. You will see there were no statistically significant difference between sources. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
  8. Study conducted by Dr. J. Arthington, University of FL was designed to compare IntelliBond C directly with a good Organic trace mineral (Availa Cu) in cattle experiencing a heavy copper antagonistic challenge 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
  9. Most nutritionists would start by looking at the use of sulfate based trace minerals within their program (line 1). This is true based on past experience and their cost relative to other trace mineral options. However, most nutritionists are fully aware that there are many negative issues associated with the use of sulfates (poor stability, antagonistic reactions in the feed and the digestive tract, handling, etc.). Given these negatives, “if price” was not an issue and given what we know about better quality organic trace minerals we would move all trace mineral supplementation to organic forms (line 2). However, cost is an issue ($5.16 versus $37.40 per cow per year) between the two programs prompting nutritionists who wish to improve the quality of the trace mineral program compromise by reducing the quality of organic trace mineral in their formulation to a level that will fit with the cost restraints of their clients (line 3). While this program does improve the quality of the program, it still relies heavily on the use of sulfates to meet the trace mineral specification of the cow. Effectively, you are almost tripling the cost of the trace mineral program to replace 25% of the total level of mineral being supplied to the cow. Hydroxy trace minerals provide you with the cost effective ability to replace all of the sulfate and organic trace minerals in the program with IntelliBond C, Z and M, (line 4) thereby providing the cow with a trace mineral program that would be equivalent or better than a total replacement with organic trace mineral but at a price point that is approximately 12% less than the combo (line 3). This is the unique value that only IntelliBond trace minerals can provide, significantly improved bioavailability at a cost point that will reduce trace mineral fed costs in program utilizing an organic trace mineral in combination with sulfates. Line 5 represents a program being utilized by nutritionists as a way to take advantage of IntelliBond’s increased bioavailability over sulfates (given the fact that we are replacing 75% of the sulfate based trace mineral with hydroxy trace minerals you are significantly increasing the level of trace mineral bioavailability in the diet, giving you the option to reduce the amount of hydroxy trace mineral being fed, with no negative impact on cow productivity / well being. Obviously this allow for a further reduction in cost per cow and improvement in environmental compliance. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
  10. Comprehensive transition cow study conducted with Dr. Tom Overton utilizing cows from the Cornell University research herd. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
  11. IntelliBond supplemented cows quickly allowed for an increase in fat corrected milk production above and beyond the combo cows and cows supplemented with just sulfates 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
  12. The table summarizes the actual tabular results of the study for all production parameters. While there were no direct treatment effects, IntelliBond supplemented cows did produce statistically significant increased in milk, fat, lactose and fat corrected milk above and beyond sulfate only and combo cows. It was also observed that cows receiving IntelliBond trace minerals lost less BW than the other two treatments during both the pre and postpartum periods. There were no statistical difference in SCC, locomotion or other health related parameters through out the study. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
  13. IntelliBond treated cattle were more effective at reducing TBAR concentration indicating a lower level of antioxidant activity in the cow, generally indicating a higher cow health status within the cows receiving IntelliBond. This was further confirmed by lower TAC levels in the cow. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
  14. The above table summarized the economic results of the study based on the NE milk order pricing grid as of 12/31/11. Looking at the comparison of program costs (last row) with incremental value gained from the use of IntelliBond (first row) you will quickly see that the use on IntelliBond produced a significant ROI over either the cows receiving straight sulfate minerals or the cows receiving the combination (75% sulfate / 25% organic). 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
  15. Given the combination of improved bio efficacy and lower cost, IntelliBond products provide nutrition professionals with the opportunity to finally get rid of all inorganic trace minerals in the ration and higher priced organics, replacing both with a constant source of highly bioavailable race minerals designed to increase trace mineral absorption, thereby driving animal productivity and health at potentially lower levels of trace miner.al inclusion in the diet 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
  16. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt