This document summarizes IntelliBond Trace Minerals, a manufacturer and supplier of trace mineral nutrition for livestock. Key points include:
- IntelliBond uses fully defined crystalline structures that are highly potent, non-hygroscopic, and not soluble above pH 4.0, allowing for complete rumen bypass and increased trace mineral absorption.
- Studies show IntelliBond provides greater stability of vitamins and other essential nutrients in feeds compared to sulfates and organics. IntelliBond also has reduced cost per unit of metal versus organics.
- Research demonstrates IntelliBond has similar or greater bioavailability than other trace mineral sources and supports rumen bypass, antioxidant effects, and performance
Almac Protein Ligation Technology Webinar Presentation 12 09-2012Susan1Beattie
The document describes Almac's protein ligation technology for site-specific conjugation and modification of recombinant proteins. Key points:
- The technology uses intein-mediated protein splicing to produce proteins with a C-terminal hydrazide, enabling chemoselective conjugation via hydrazone or oxime bond formation.
- Examples demonstrate fluorescent labeling and PEGylation of proteins while maintaining biological activity, including interferon alpha and beta and a single domain antibody.
- PEGylation using the site-specific method results in improved pharmacokinetics compared to non-specific PEGylation, with activity retained close to the unmodified proteins.
- The technology provides a versatile platform for therapeutic applications including drug delivery
The document describes an IIT Bombay patented soil biotechnology (SBT) system for treating wastewater. SBT uses formulated media, culture, and additives to biologically purify water. It provides primary, secondary, and tertiary treatment in a single facility that is simple to operate and maintain. The technology has been implemented for sewage treatment, effluent treatment from industries like distilleries, and for greywater recycling. An example is presented showing how SBT was used to treat and recycle over 720 cubic meters per day of distillery process condensate.
This document summarizes the work of Prof. H.S. Shankar of the Department of Chemical Engineering at IIT Bombay on soil biotechnology. Some key points:
1) Soil biotechnology uses microorganisms and biochemical processes to purify water, process solid wastes for agriculture, and enhance crop yields in a sustainable manner.
2) Experiments show that multi-stage water purification systems using microbial reactors can effectively treat sewage water.
3) Earthworms and other organisms play an important role in processing organic wastes in the soil and increasing nutrient availability for plant growth.
4) Studies of the carbon cycle, nitrogen fixation, photosynthesis, and other biochemical reactions
Presentation of Henrique M. Baudel for the Workshop on Hydrolysis Route for Cellulosic Ethanol from Sugarcane.
Apresentação de Henrique M. Baudel realizada no "Workshop on Hydrolysis Route for Cellulosic Ethanol from Sugarcane"
Date / Data : February 10 - 11th 2009/
10 e 11 de fevereiro de 2009
Place / Local: Unicamp, Campinas, Brazil
Event Website / Website do evento: http://www.bioetanol.org.br/workshop1
This document outlines 10 hot topics in biomanufacturing: 1) product mix and business models in biopharma, 2) high-titer processes in mammalian cell culture, 3) downstream processing being driven by mass rather than volume, 4) facility fit considerations, 5) cost of goods and price margin pressures, 6) biosimilars and biobetters, 7) offshore manufacturing, 8) technology platforms and implications of high titer, 9) emerging technologies for biomass removal and contaminant clearance, and 10) single-use technologies and integrated processing. The speaker concludes by emphasizing the need for new perspectives and solutions to address challenges in biomanufacturing.
New Developments Through Microscopic Reconstruction of the Nature of Fluidize...Masayuki Horio
Presentation was made at AIChE Particle Technology Forum as an Award Lecture.
After a brief review of achievements of fluidization engineering over decades, a discussion is made on one of the latest issues for applications in material industries as well as for the improvements in reliability of many fluidization processes, i.e., granulation and defluidization issues.
2.1 Background
For a long period, phenomena associated with agglomerating fluidization have been treated with complete empiricism and scientific lights were shed seldom on them. It was, however, natural because the basic intention of fluidization has long been the better gas and solid contacting and, accordingly, agglomeration has been only one of unwanted side effects, which, once technically avoided, tend to be forgotten. At the same time, knowledge on elementary processes that should be relevant to agglomerating fluidization, e.g., bubble characteristics, forces acting among fluidized particles, surface characteristics of solids etc., was only gradually established during the last decades.
Defluidization/agglomeration issues are, however, quite significant in a majority of fluidization processes probably except for gas-to-gas catalytic processes. In polyolefin processes agglomeration due to softening of plastic particles in local hot spots should be avoided. In a polyolefin reactor it has been confirmed by a DEM simulation of Kaneko et al. (1998) that a stable solid circulation does not help removing the heat of polymerization. Instead, a solid motion induced by the always-fluctuating bubbling action is necessary as shown in Fig. 3.
Ash melting and agglomeration, which finally causes defluidization, limits the operating temperature and pressure of pressurized fluidized bed combustion (PFBC) or gasification (PFBG). Figure 4 shows the so-called "sinter eggs" formed in a FBC boiler that is close to those found in AEP Tidd PFBC. Sinter egg/grain formation is again experienced recently in a commercial scale PFBC in Japan.
1) The document describes a study to develop a continuous gradient assisted hydrophobic interaction chromatography process using Simulated Moving Bed (SMB-HIC) for the purification of recombinant streptokinase (rec-stk) from E. coli cell homogenate.
2) Key steps included producing and purifying rec-stk, estimating adsorption isotherms, designing a 3-zone open loop SMB system using a scanning program, and experimental realization of the SMB process.
3) Simulation studies were conducted to identify separation regions using equilibrium theory and stage models, and operating points were selected for experimental validation of the continuous purification of rec-stk via SMB-HIC.
1) Lead is an industrially useful metal but poses health risks through occupational and environmental exposure. It has no biological role in the human body.
2) Workers in industries like battery manufacturing and silver jewelry making face dangers of lead toxicity through occupational exposure. Exposure is often unregulated in small-scale units in developing countries.
3) The study assessed lead toxicity in small-scale industry workers in India and evaluated biochemical markers for screening occupational exposure. It found that urinary delta-aminolevulinic acid was the most suitable, sensitive, specific and cost-effective marker for screening low levels of lead toxicity.
Almac Protein Ligation Technology Webinar Presentation 12 09-2012Susan1Beattie
The document describes Almac's protein ligation technology for site-specific conjugation and modification of recombinant proteins. Key points:
- The technology uses intein-mediated protein splicing to produce proteins with a C-terminal hydrazide, enabling chemoselective conjugation via hydrazone or oxime bond formation.
- Examples demonstrate fluorescent labeling and PEGylation of proteins while maintaining biological activity, including interferon alpha and beta and a single domain antibody.
- PEGylation using the site-specific method results in improved pharmacokinetics compared to non-specific PEGylation, with activity retained close to the unmodified proteins.
- The technology provides a versatile platform for therapeutic applications including drug delivery
The document describes an IIT Bombay patented soil biotechnology (SBT) system for treating wastewater. SBT uses formulated media, culture, and additives to biologically purify water. It provides primary, secondary, and tertiary treatment in a single facility that is simple to operate and maintain. The technology has been implemented for sewage treatment, effluent treatment from industries like distilleries, and for greywater recycling. An example is presented showing how SBT was used to treat and recycle over 720 cubic meters per day of distillery process condensate.
This document summarizes the work of Prof. H.S. Shankar of the Department of Chemical Engineering at IIT Bombay on soil biotechnology. Some key points:
1) Soil biotechnology uses microorganisms and biochemical processes to purify water, process solid wastes for agriculture, and enhance crop yields in a sustainable manner.
2) Experiments show that multi-stage water purification systems using microbial reactors can effectively treat sewage water.
3) Earthworms and other organisms play an important role in processing organic wastes in the soil and increasing nutrient availability for plant growth.
4) Studies of the carbon cycle, nitrogen fixation, photosynthesis, and other biochemical reactions
Presentation of Henrique M. Baudel for the Workshop on Hydrolysis Route for Cellulosic Ethanol from Sugarcane.
Apresentação de Henrique M. Baudel realizada no "Workshop on Hydrolysis Route for Cellulosic Ethanol from Sugarcane"
Date / Data : February 10 - 11th 2009/
10 e 11 de fevereiro de 2009
Place / Local: Unicamp, Campinas, Brazil
Event Website / Website do evento: http://www.bioetanol.org.br/workshop1
This document outlines 10 hot topics in biomanufacturing: 1) product mix and business models in biopharma, 2) high-titer processes in mammalian cell culture, 3) downstream processing being driven by mass rather than volume, 4) facility fit considerations, 5) cost of goods and price margin pressures, 6) biosimilars and biobetters, 7) offshore manufacturing, 8) technology platforms and implications of high titer, 9) emerging technologies for biomass removal and contaminant clearance, and 10) single-use technologies and integrated processing. The speaker concludes by emphasizing the need for new perspectives and solutions to address challenges in biomanufacturing.
New Developments Through Microscopic Reconstruction of the Nature of Fluidize...Masayuki Horio
Presentation was made at AIChE Particle Technology Forum as an Award Lecture.
After a brief review of achievements of fluidization engineering over decades, a discussion is made on one of the latest issues for applications in material industries as well as for the improvements in reliability of many fluidization processes, i.e., granulation and defluidization issues.
2.1 Background
For a long period, phenomena associated with agglomerating fluidization have been treated with complete empiricism and scientific lights were shed seldom on them. It was, however, natural because the basic intention of fluidization has long been the better gas and solid contacting and, accordingly, agglomeration has been only one of unwanted side effects, which, once technically avoided, tend to be forgotten. At the same time, knowledge on elementary processes that should be relevant to agglomerating fluidization, e.g., bubble characteristics, forces acting among fluidized particles, surface characteristics of solids etc., was only gradually established during the last decades.
Defluidization/agglomeration issues are, however, quite significant in a majority of fluidization processes probably except for gas-to-gas catalytic processes. In polyolefin processes agglomeration due to softening of plastic particles in local hot spots should be avoided. In a polyolefin reactor it has been confirmed by a DEM simulation of Kaneko et al. (1998) that a stable solid circulation does not help removing the heat of polymerization. Instead, a solid motion induced by the always-fluctuating bubbling action is necessary as shown in Fig. 3.
Ash melting and agglomeration, which finally causes defluidization, limits the operating temperature and pressure of pressurized fluidized bed combustion (PFBC) or gasification (PFBG). Figure 4 shows the so-called "sinter eggs" formed in a FBC boiler that is close to those found in AEP Tidd PFBC. Sinter egg/grain formation is again experienced recently in a commercial scale PFBC in Japan.
1) The document describes a study to develop a continuous gradient assisted hydrophobic interaction chromatography process using Simulated Moving Bed (SMB-HIC) for the purification of recombinant streptokinase (rec-stk) from E. coli cell homogenate.
2) Key steps included producing and purifying rec-stk, estimating adsorption isotherms, designing a 3-zone open loop SMB system using a scanning program, and experimental realization of the SMB process.
3) Simulation studies were conducted to identify separation regions using equilibrium theory and stage models, and operating points were selected for experimental validation of the continuous purification of rec-stk via SMB-HIC.
1) Lead is an industrially useful metal but poses health risks through occupational and environmental exposure. It has no biological role in the human body.
2) Workers in industries like battery manufacturing and silver jewelry making face dangers of lead toxicity through occupational exposure. Exposure is often unregulated in small-scale units in developing countries.
3) The study assessed lead toxicity in small-scale industry workers in India and evaluated biochemical markers for screening occupational exposure. It found that urinary delta-aminolevulinic acid was the most suitable, sensitive, specific and cost-effective marker for screening low levels of lead toxicity.
This document discusses waste-to-energy and biogas as potential future fuel sources. It provides background on Farmatic, a company with 50 years of experience in biogas and waste water treatment. Farmatic has over 300 project references globally and sees opportunities in the US market for biogas from waste streams, agriculture, and as an advanced biofuel. Key differences between the European and US environments for biogas are noted, including factors like population density, electricity costs, available land and feedstocks, waste definitions and regulations.
The document discusses PolyCarb, a lignosulphonate chelate that is able to chelate metal ions due to hydroxyl, carboxyl, and sulphonyl groups, giving it a high cation exchange capacity. It is derived from plant sources, making the chelated micronutrients more natural than synthetic options. The document provides details on PolyCarb's chelated forms of calcium, copper, iron, magnesium, manganese, and zinc, including formulations, pH, dosage, and advantages over sulphate and EDTA forms of metals.
New LC-IR Technique To Characterize Polymeric Excipients In Pharmaceutical Fo...mzhou45
GPC-IR combined technique to characterize polymeric excipients for lot-to-lot variations and degradation/stability from thermal processing in drug formulations
The document describes tools and methods for screening compounds to find effective drugs for anxiety and depression. A typical screening process involves chemical synthesis of compounds, followed by screening assays to test binding and functional activity. Data is analyzed to select hit compounds, which then undergo further testing through ex vivo occupancy studies, in vitro ADME assays, and in vivo safety and efficacy models. Key methods discussed include radioligand binding assays to test if compounds compete for the same binding site as labeled ligands, and saturation binding assays to determine receptor affinity (Kd) and maximum binding (Bmax). Comparing results between species and receptor subtypes can provide insights into a compound's potential and selectivity.
This document describes a new technology developed by PolyBatics Ltd for displaying proteins at high density on the surface of bioplastic beads. The technology utilizes the natural ability of many microbes to produce intracellular polyester granules onto which proteins can be displayed. PolyBatics has demonstrated this platform can be used for applications such as separations, diagnostics, vaccine delivery and biocatalysis. The high density display of functional proteins on the surface of the biodegradable and recyclable beads provides advantages over traditional immobilization methods.
The document describes metabolic engineering experiments to optimize Escherichia coli for efficient conversion of glucose to pyruvate. Key modifications included deleting the poxB gene encoding pyruvate oxidase to increase pyruvate production, and decreasing pyruvate dehydrogenase levels through deletion of pdhR and replacing its promoter. Initial experiments successfully deleted poxB but found contamination in strains. Batch cultures showed highest pyruvate concentrations of 6.14 g/L for strain R1 ΔpoxB. While results were mixed, the work provided experience with laboratory techniques and engineering a microbial strain through a full project.
17 leco's citius high res lcms-faster results-fewer runs david schlegel - lec...CPSA-2012_5-Minutes-Fame
The document discusses LECO's Citius High Res LC/MS instrument. It can (1) produce qualitative and quantitative results from a single run on the same instrument, (2) obtain excellent results faster with fewer runs through high resolution time-of-flight mass spectrometry, and (3) provide researchers a single instrument that can produce high quality results in a single analysis through post-acquisition data processing.
Phosphite treatment:
- Significantly reduces the incidence of Microdochium nivale in various grass species and enhances fungicide suppression of the pathogen.
- Inhibits M. nivale mycelial growth, conidial germination, and disrupts hyphal morphology in vitro.
- Is rapidly absorbed and translocated in grass tissues where it accumulates.
Trace minerals are needed in small amounts but are essential for many bodily functions. They include iron, zinc, iodine, selenium, fluoride, manganese, molybdenum, chromium, and others. Trace mineral deficiencies can impair growth, development, immunity and cognitive function, while toxicities of some like iron, fluoride, and iodine can also negatively impact health. Food content of trace minerals depends on soil mineral content where the food was grown.
Minerals are inorganic elements essential for human nutrition. Fourteen minerals are required for proper body function and play key roles in health. They include calcium, phosphorus, magnesium, iron, zinc and others. Minerals help with chemical reactions in cells, muscle contraction, nerve transmission and more. They are divided into major minerals needed in larger amounts daily and trace minerals needed in smaller amounts. Maintaining proper mineral balance is important for overall health and prevention of deficiencies or toxicities.
Minerals are inorganic nutrients that are essential for the proper functioning of the human body. There are two types of minerals - macro minerals which are needed in large amounts, and trace minerals which are needed in small amounts. Minerals play important roles such as building bones and teeth, facilitating energy production and oxygen transport, and maintaining electrolyte and acid-base balance. Common minerals include calcium, iron, magnesium, phosphorus, potassium, sodium, and zinc. Deficiencies of minerals can lead to conditions like anemia, osteoporosis, and rickets, while excessive intake of some minerals may also cause health issues. We rely on obtaining minerals through our diet and supplements to support overall health and wellness.
The document discusses several trace minerals including zinc, selenium, copper, and boron. It provides details on their functions, dietary sources, requirements and interactions in the body. Trace minerals serve as cofactors for enzymes and play important roles in various physiological processes like immune function, membrane stability, and antioxidant protection. Maintaining adequate intake of trace minerals through the diet is important for overall health.
This document provides an overview of minerals that are essential for human nutrition including sources, daily requirements, absorption, functions, regulation, clinical manifestations of deficiencies and toxicities. It describes macro minerals such as calcium, phosphorus, magnesium, and sodium as well as trace minerals including iron, zinc, copper and iodine. The seminar aims to describe the roles of these minerals in various metabolic processes and health.
Minerals are inorganic elements needed by the body for functions like building tissues, regulating fluids, and assisting in bodily processes. Major minerals like calcium, phosphorus, and potassium are required in amounts greater than 100mg daily, while trace minerals such as iron, iodine, and zinc are needed in lesser amounts. The document provides details on common food sources, functions, and deficiency/toxicity symptoms for each major and trace mineral.
Minerals are essential for normal growth and maintenance of the body.
Major elements : Requirement >100 mg /day
Trace Elements : Requirement <100mg/day
Some are necessary for the body but their exact functions are not known.
Ex.: Chromium, Nickel, Bromide, Lithium, Barium
Non-Essentials : seen in tissues. Contaminants in food stuffs.
Ex.: Rubedium, Silver, Gold, Bismuth
Toxic : should be avoided.
Ex.: Aluminium, Lead, Cadmium, Mercury
This document provides an overview of trace elements, including their classification as essential, probably essential, or non-essential. It discusses several important trace elements in depth, including iron, zinc, copper, chromium, fluoride, iodine, manganese, molybdenum, and selenium. For each element, it covers their functions, dietary sources, deficiency and toxicity symptoms, and treatment as needed. It also briefly mentions other trace elements like vanadium, tin, nickel, boron, lithium, and xenon.
1) Minerals are naturally formed solid substances with a crystal structure that are not alive and have a definite volume and shape.
2) All minerals share characteristics of being formed by natural processes, having a unique chemical makeup, and being made up of particles arranged in a repeating crystal pattern.
3) Minerals are grouped based on their chemical composition, with the most abundant group being silicates containing oxygen and silica like quartz and mica.
This document discusses vitamins, minerals, and water. It explains that vitamins and minerals regulate body functions and must be ingested daily through foods like fruits, vegetables, and whole grains. Dark leafy greens contain the most vitamins and minerals. Water carries nutrients through the body, regulates temperature, and prevents dehydration. We should drink half our body weight in ounces of water daily. The document then provides details on specific vitamins (A, D, E, K, C, B vitamins) and minerals, their functions, sources, deficiencies, and toxicities.
This document discusses waste-to-energy and biogas as potential future fuel sources. It provides background on Farmatic, a company with 50 years of experience in biogas and waste water treatment. Farmatic has over 300 project references globally and sees opportunities in the US market for biogas from waste streams, agriculture, and as an advanced biofuel. Key differences between the European and US environments for biogas are noted, including factors like population density, electricity costs, available land and feedstocks, waste definitions and regulations.
The document discusses PolyCarb, a lignosulphonate chelate that is able to chelate metal ions due to hydroxyl, carboxyl, and sulphonyl groups, giving it a high cation exchange capacity. It is derived from plant sources, making the chelated micronutrients more natural than synthetic options. The document provides details on PolyCarb's chelated forms of calcium, copper, iron, magnesium, manganese, and zinc, including formulations, pH, dosage, and advantages over sulphate and EDTA forms of metals.
New LC-IR Technique To Characterize Polymeric Excipients In Pharmaceutical Fo...mzhou45
GPC-IR combined technique to characterize polymeric excipients for lot-to-lot variations and degradation/stability from thermal processing in drug formulations
The document describes tools and methods for screening compounds to find effective drugs for anxiety and depression. A typical screening process involves chemical synthesis of compounds, followed by screening assays to test binding and functional activity. Data is analyzed to select hit compounds, which then undergo further testing through ex vivo occupancy studies, in vitro ADME assays, and in vivo safety and efficacy models. Key methods discussed include radioligand binding assays to test if compounds compete for the same binding site as labeled ligands, and saturation binding assays to determine receptor affinity (Kd) and maximum binding (Bmax). Comparing results between species and receptor subtypes can provide insights into a compound's potential and selectivity.
This document describes a new technology developed by PolyBatics Ltd for displaying proteins at high density on the surface of bioplastic beads. The technology utilizes the natural ability of many microbes to produce intracellular polyester granules onto which proteins can be displayed. PolyBatics has demonstrated this platform can be used for applications such as separations, diagnostics, vaccine delivery and biocatalysis. The high density display of functional proteins on the surface of the biodegradable and recyclable beads provides advantages over traditional immobilization methods.
The document describes metabolic engineering experiments to optimize Escherichia coli for efficient conversion of glucose to pyruvate. Key modifications included deleting the poxB gene encoding pyruvate oxidase to increase pyruvate production, and decreasing pyruvate dehydrogenase levels through deletion of pdhR and replacing its promoter. Initial experiments successfully deleted poxB but found contamination in strains. Batch cultures showed highest pyruvate concentrations of 6.14 g/L for strain R1 ΔpoxB. While results were mixed, the work provided experience with laboratory techniques and engineering a microbial strain through a full project.
17 leco's citius high res lcms-faster results-fewer runs david schlegel - lec...CPSA-2012_5-Minutes-Fame
The document discusses LECO's Citius High Res LC/MS instrument. It can (1) produce qualitative and quantitative results from a single run on the same instrument, (2) obtain excellent results faster with fewer runs through high resolution time-of-flight mass spectrometry, and (3) provide researchers a single instrument that can produce high quality results in a single analysis through post-acquisition data processing.
Phosphite treatment:
- Significantly reduces the incidence of Microdochium nivale in various grass species and enhances fungicide suppression of the pathogen.
- Inhibits M. nivale mycelial growth, conidial germination, and disrupts hyphal morphology in vitro.
- Is rapidly absorbed and translocated in grass tissues where it accumulates.
Trace minerals are needed in small amounts but are essential for many bodily functions. They include iron, zinc, iodine, selenium, fluoride, manganese, molybdenum, chromium, and others. Trace mineral deficiencies can impair growth, development, immunity and cognitive function, while toxicities of some like iron, fluoride, and iodine can also negatively impact health. Food content of trace minerals depends on soil mineral content where the food was grown.
Minerals are inorganic elements essential for human nutrition. Fourteen minerals are required for proper body function and play key roles in health. They include calcium, phosphorus, magnesium, iron, zinc and others. Minerals help with chemical reactions in cells, muscle contraction, nerve transmission and more. They are divided into major minerals needed in larger amounts daily and trace minerals needed in smaller amounts. Maintaining proper mineral balance is important for overall health and prevention of deficiencies or toxicities.
Minerals are inorganic nutrients that are essential for the proper functioning of the human body. There are two types of minerals - macro minerals which are needed in large amounts, and trace minerals which are needed in small amounts. Minerals play important roles such as building bones and teeth, facilitating energy production and oxygen transport, and maintaining electrolyte and acid-base balance. Common minerals include calcium, iron, magnesium, phosphorus, potassium, sodium, and zinc. Deficiencies of minerals can lead to conditions like anemia, osteoporosis, and rickets, while excessive intake of some minerals may also cause health issues. We rely on obtaining minerals through our diet and supplements to support overall health and wellness.
The document discusses several trace minerals including zinc, selenium, copper, and boron. It provides details on their functions, dietary sources, requirements and interactions in the body. Trace minerals serve as cofactors for enzymes and play important roles in various physiological processes like immune function, membrane stability, and antioxidant protection. Maintaining adequate intake of trace minerals through the diet is important for overall health.
This document provides an overview of minerals that are essential for human nutrition including sources, daily requirements, absorption, functions, regulation, clinical manifestations of deficiencies and toxicities. It describes macro minerals such as calcium, phosphorus, magnesium, and sodium as well as trace minerals including iron, zinc, copper and iodine. The seminar aims to describe the roles of these minerals in various metabolic processes and health.
Minerals are inorganic elements needed by the body for functions like building tissues, regulating fluids, and assisting in bodily processes. Major minerals like calcium, phosphorus, and potassium are required in amounts greater than 100mg daily, while trace minerals such as iron, iodine, and zinc are needed in lesser amounts. The document provides details on common food sources, functions, and deficiency/toxicity symptoms for each major and trace mineral.
Minerals are essential for normal growth and maintenance of the body.
Major elements : Requirement >100 mg /day
Trace Elements : Requirement <100mg/day
Some are necessary for the body but their exact functions are not known.
Ex.: Chromium, Nickel, Bromide, Lithium, Barium
Non-Essentials : seen in tissues. Contaminants in food stuffs.
Ex.: Rubedium, Silver, Gold, Bismuth
Toxic : should be avoided.
Ex.: Aluminium, Lead, Cadmium, Mercury
This document provides an overview of trace elements, including their classification as essential, probably essential, or non-essential. It discusses several important trace elements in depth, including iron, zinc, copper, chromium, fluoride, iodine, manganese, molybdenum, and selenium. For each element, it covers their functions, dietary sources, deficiency and toxicity symptoms, and treatment as needed. It also briefly mentions other trace elements like vanadium, tin, nickel, boron, lithium, and xenon.
1) Minerals are naturally formed solid substances with a crystal structure that are not alive and have a definite volume and shape.
2) All minerals share characteristics of being formed by natural processes, having a unique chemical makeup, and being made up of particles arranged in a repeating crystal pattern.
3) Minerals are grouped based on their chemical composition, with the most abundant group being silicates containing oxygen and silica like quartz and mica.
This document discusses vitamins, minerals, and water. It explains that vitamins and minerals regulate body functions and must be ingested daily through foods like fruits, vegetables, and whole grains. Dark leafy greens contain the most vitamins and minerals. Water carries nutrients through the body, regulates temperature, and prevents dehydration. We should drink half our body weight in ounces of water daily. The document then provides details on specific vitamins (A, D, E, K, C, B vitamins) and minerals, their functions, sources, deficiencies, and toxicities.
1. We are Growing!
“IntelliBond Trace Minerals”
Shattering Trace Mineral Perceptions!
1
2. Micronutrients
• Second largest manufacturer and supplier of trace mineral
nutrition in NA
• Dedicated only to trace mineral nutrition in livestock
• Third party auditors certify high product quality
– HAACP, ISO 9002, Safe Feed, Safe Food, FAMIQs
• All products American made in Indianapolis, In
2
3. IntelliBond Trace Minerals:
• Fully defined crystalline structures
– High trace metal potency
– Non-hygroscopic
– Not soluble above pH 4.0
• Complete rumen by-pass
• Increased trace metal absorption
• Greater stability of essential nutrients (vitamins, etc.)
3
4. Trace Mineral Nutrition in Ruminants
• Sulfates / Oxides used from the 50’s
– Highly reactive in feeds and the rumen
– Lower cost per unit of metal
• Organics developed in the 1970’s and 80’s
– Improved stability in feeds
– Better trace mineral absorption
– High cost per unit of metal
• Hydroxy trace minerals (IntelliBond®)
– No adverse impact on essential nutrients in feeds
– Complete rumen bypass of essential metals
– Increased trace metal absorption in the small intestine
– Reduced cost per unit of metal
4
5. Smart Release Crystals -
Protect Essential Nutrients Present in Premixes & Feeds
Mash, Broiler diet with 3.6% added fat
70 Control (18 ppm)
Cu sulfate (200 ppm)
Feed Vitamin E (mg/kg)
60 IntelliBond C (200 ppm)
50
40
*
30 * Feed levels correspond to lower
liver and plasma Vit. E in CuSO4
20 32% loss in
* * fed chicks (p<0.05).
Vitamin E activity
10 at 10 days
0
0 10 20 30 40
Storage time (d)
* Within a time point, mean is significantly different from Control (p<0.05).
5
Data adapted from Lu et al., 2010. Biol. Trace Elem. Res. 138:181-9.
6. Impact of Vitamin E Degradation
Assumptions:
• Cow receiving 750 IU Vit. E per day in a 3.0 lb supplement, that also delivers -
• 15 ppm copper 75 ppm Zinc and 45 ppm manganese in final ration
• Supplement specs = 0.055% Vit E, 243 ppm Cu, 1,213 ppm Zn & 728 ppm Mn
• Premix sustains a 20% loss in its Vit. E content within two week storage period
Results:
• At 750 IUs per day, cow consumes .55 Kgs per year, E 50% ($9.30 Cow/yr)
• 750 IUs / 50% = 1,500 mg/d E 50% X 365 days = .548 Kgs / yr x $17.00 Kg)
• 20% loss = .11 Kg Vit. E 50% resulting in a loss of $1.86 / cow in Vit. E activity)
Pay back: (Greater than 4.0:1)
• Cost of 15 ppm Cu from CuSO4 = .48 Kgs ($1.35) cow per year
• Cost of 15 ppm from IntelliBond C = .21 Kgs ($1.80) cow per year
6
• Incremental cost to replace copper sulfate is $.45 cow per year
7. Smart Release Crystals -
Support Rumen / Abomasum By-Pass
O O
S
O O
X
H2O OH2
Cu Typical Organic Trace Mineral
H2O OH2
Inorganic trace mineral
IntelliBond C Cl
Defined Crystal Matrix OH Stretched Covalent Bond
HO OH HO OH
Cu Cu
HO OH HO OH
At a pH Covalent Bond
of < 3.5
7 Cl Soluble form Cl
8. Smart Release Crystals –
Increase Trace Mineral Intestinal Absorption
IntelliBond C Relative Bioavailability In Cattle
Trial Design:
Dependent Variable Copper RBV P=
•98 day depletion period source
•60 steers
•3 X 2 factorial setup Plasma Cu CuSO4 100 0.07
•10 steers / treatment IBc 132
•Trts @ 0, 5 & 10 ppm Cu
Liver Cu CuSO4 100 0.04
•5 ppm Mo & .15 % S
IBc 196
J.W. Spears et al. N.C. State University
Trial Design:
•16 yearling steers IntelliBond Z Relative Bioavailability In Cattle
•Depleted 14 days 2X
Dependent Variable Zinc RBV P=
(basal @ 25 ppm Zn) Source Increase
•Treatments: in RBV
Zinc sulfate @ 25 ppm Absorbed Zn ZnSO4 100 0.01
IntelliBond Z @ 25 ppm
IBz 205
•Seven day adaptation
•Five day total collection
Retained Zn ZnSO4 100 0.01
Urine & fecal output
IBz 204
Feed in & Orts
J.W. Spears et al. N.C. State University
9. IntelliBond C vs. OTM In Broilers:
• Ross birds grown to 56 days of age
• Floor pens, w/ used litter (.8 ft2 / bird)
• 10 replicates of each treatment (25 birds per pen) Results:
Added Copper Levels (ppm)
•All sources improved F/G
Test Materials Starter Grower Finisher •No significant difference at any point
Control (no added Cu) None None None
TBCC 25 25 25
TBCC 50 50 50
TBCC 75 75 75
TBCC 100 100 100
TBCC 125 125 125
TBCC 150 150 150
TBCC 175 175 175
Cu Metal Proteinate 25 25 25
Cu Metal Proteinate 50 50 50
Cu Metal Proteinate 75 75 75
Cu Metal Proteinate 100 100 100
Cu Amino Acid Complex 25 25 25
Cu Amino Acid Complex 50 50 50
Cu Amino Acid Complex 75 75 75
Cu Amino Acid Complex 100 100 100 A.H. Pharma Research Institute
10. IntelliBond C vs. Cu Lysine in Lambs
Study Design: • Treatments:
•50 wether lambs w/ initial BW of 23.8 Kg – Control: – no supplemental copper
•10 lambs / trt, individually housed in wood pens – Trt 2: 10 ppm Cu from Cu Lysine
•All lambs on common diet first 3 weeks – Trt 3: 20 ppm Cu from Cu Lysine
•At week 4, lambs weighed & blocked into 5 trts – Trt 4: 10 ppm Cu from IntelliBond C
•60 day evaluation – Trt 5: 20 ppm Cu from IntelliBond C
• Orts were recorded and feed intake adj’d weekly • Measurements:
– Blood drawn at days 0, 30 and 60
– All lambs harvested at 60 days, livers
IntelliBond C and Cu-lysine were of similar collected and assayed
availability when fed to finishing lambs.
Treatments Contrast P Value
Cu Cu IB C Cont 10 vs IB C IB C
Lysine Lysine IB C 20 Vs 20 vs Lys vs Lys
Item Control 10 ppm 20 ppm 10 ppm ppm SEM Cu ppm 10 20
Plasma Cu (mg/l)
Day 0 0.74 0.84 0.78 0.77 0.81 0.02 0.29 0.82 0.35 0.67
Day 60 0.89 0.96 1.08 1.03 1.09 0.03 0.02 0.11 0.32 0.89
Liver Cu (mg/Kg) 45.4 278.5 373.8 297.0 405.1 29.8 0.01 0.01 0.47 0.23
2011. Biol. Trace Elements Res. Vol 144. pp 570 - 579. 10
11. IntelliBond C vs. Availa Cu in Yearlings
Study Design: IntelliBond C and Availa Cu were
•24 Brahman crossbred steers of similar availability when fed to
•72 day study yearling steers
•4 Trt / 6 pens per
•Trt’s All cattle on 10 ppm Cu Availa Cu
– 10 ppm Cu – Availa Cu
– 10 ppm Cu – IntelliBond C
– 15 ppm Cu Availa Cu + 15 ppm Cu IntelliBond C
– 30 ppm Cu IntelliBond C IBC/
•Free-choice access to star-grass hay IBC Availa C IBC Availa C
•Fed molasses supplement with Trts Day 10 ppm 10 ppm 30 ppm 30 ppm
•Measurements – Liver copper at:
0 146 161 125 158
– 0, 24, 48 & 72 days
24 136 151 182 163
48 143 145 195 151
72 166 157 226 213
*Liver Cu expressed as PPM Dry Matter
J. Anim. Sci. 2007 85:871-876
J. Arthington, Ph.D / J. Spears, Ph.D.
13. Cornell – Transition Cow Study Design
Design:
• Transition cow study (21 days prepartum through 84 days post partum)
• 60 total cows – 3 treatments of 20 cows each
– Cows individually fed (tie stall)
• Cows milked 2x / day, within the Cornell milking facility
• Milk weighed at each milking
• Separate composite milk sample taken from AM / PM milkings weekly
Treatments: (zinc @ 60 ppm; Mn @ 40 ppm & Cu @ 15 ppm)
– All sulfate
– 75% sulfate / 25% Availa Zn, Mn & Cu
– All IntelliBond (hydroxy trace minerals)
Measurements:
• All performance parameters
13 • Cow well being & health T. Overton, Ph.D, Cornell University, Ithaca, NY
16. Cornell Transition: Oxidative Stress
Treatments P-value
Item ITM ITM/OTM IntelliBond SEM Trt Wk Trt x Wk
TBARS, uM (Thiobarbituric acid-reactive substances)
Whole study 2.11A 1.98AB 1.95B 0.07 0.16 <.01 0.40
Prepartum 1.47 1.49 1.38 0.06 0.37 0.16 0.74
Postpartum 2.26 2.11 2.10 0.09 0.29 <.01 0.23
TAC, mM (Total antioxidant capacity)
Whole study 2.14a 2.07ab 1.93b 0.07 0.09 <.01 0.94
Prepartum 2.09a 1.93ab 1.84b 0.08 0.10 0.01 0.86
Postpartum 2.16a 2.09ab 1.95b 0.08 0.12 <.01 0.88
A,B
Least square means within row with different upper-case superscript tend to differ (P < 0.1)
16
a,b
Least square means within row with different lower-case superscript differ (P < 0.05)
17. Projected Economics:
Parameter Inorganic Combo IntelliBond IntelliBond Incremental Value
Lbs (value) (ITM/OTM) Lbs (value) ITM vs. IB Combo
Lbs (value) vs. IB
Milk (lbs) 99.9 ($19.17) 100.8 ($19.34) 102.7 ($19.71) $.54 cow/d $.37 cow/d
Fat (lbs) 3.26 ($5.68) 3.20 ($5.57) 3.28 ($5.71) $.03 cow/d $.14 cow/d
Protein (lbs) 2.95 ($9.78) 2.93 ($9.71) 3.02 ($10.00) $.22 cow/d $.29 cow/d
Lactose (lbs) 4.72 ($2.21) 4.76 ($2.23) 4.81 ($2.25) $.04 cow/d $.02 cow/d
Program Cost $.0130 cow/d $.0325 cow/d $.0295 cow/d $.0165 cow/d -$.003 cow/d
Milk pricing based on N.E. Milk Order as of Dec. 31st, 2011
17
18. Key Value Points:
• Fully defined crystalline structure
• Increased metal potency
• Maintains essential nutrient potency in the supplement / feed
• Rumen By-Pass
– Increased rumen function (DM digestibility)
• Crystalline structure provides for sustained release and improved
absorption of the mineral in the intestinal tract
• Increased relative bioavailability (equivalent or better than best OTMs)
– Greater essential mineral absorption drives cow productivity and health
– Ability to reduce total mineral fed, reducing cost & environmental compliance
The only cost effective means to completely replace Trace
Mineral sulfate / oxides forms in the diet
18
Recently completed plant expansion for the manufacture of IntelliBond Z and M represents a significant investment in Micronutrients’ commitment to the North American dairy business. The plant is located in Indianapolis, IN and is open for your inspection should you travels bring you to Indianapolis. Micronutrients’ IntelliBond C manufacturing plant is located at the same site. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt The use of IntelliBond products, especially the replacement of Cu sulfate given its classification as a pro oxidant (Ref: Wikipedia) can significant reduce the destruction of essential nutrients (Vitamins, DFMs, etc.) in feeds and premixes. The above study involved a layer mash feed containing 3.6% added fat that was stored in a bin at approximately 70 degrees F. You will note that the feed containing IntelliBond C did not promote oxidation (present in the Cu Sulfate trt.). In fact results indicated that over 30% of the tocopherol was destroyed in the first 30 days. We find this same type of situation occurring in the dairy industry given the use of various by products within various commodity blends / supplements used to also deliver the vitamins and trace minerals in the same package
As a follow up to the prior slide this slide is designed to highlight the negative economic impact of sulfates on vita E stability in a premix. The slide is designed to quickly show the effect of a 20% loss in Vit. E activity within a supplement being fed at the rate of 1 lbs / hd / day. This can result in a loss of $1.65 per hd/yr. When you calculate the incremental cost of moving from copper sulfate to IntelliBond C of $.40 per cow per year, just the reduction in lost Vit e activity provided a ROI of 4:1. Please note the trace mineral levels highlighted under assumptions assumes a ration that is designed to deliver 15 ppm Cu, 80 ppm Zn and 40 ppm Mn and is delivered to the cow in the 1 lbs supplement. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
All IntelliBond Trace minerals are rumen by pass allowing them to escape interaction with the many antagonists that routinely affect trace minerals stability and bioavailability in the rumen. Given their ability to resist going into solution at a pH above 3.5 they are naturally By-passed to the abomasum with limited negative impact. Each IntelliBond TM form incorporates a covalent bond between its metal and the OH group. Like better quality organic trace minerals the covalent bond structure protects the metal as it makes it way from the rumen to the small intestine. In addition to the functionality of its covalent bond all IntelliBond products exist in a crystalline matrix, which provides an additional level of protection when the crystal is moving through the rumen, protecting it from antagonisms. Once IntelliBond crystals enter the abomasum, they encounter an area of low pH allowing the crystals to move from their crystalline form into solution, still stabilized by the presence of the covalent bond between the metal and the OH groups. Once in solution the IntelliBond metals are now available for absorption, following their transit to the small intestine, allowing for increased bioavailability of the metal to the animal. Crystal References: IntelliBond C - http://en.wikipedia.org/wiki/Atacamite IntelliBond Z - http://en.wikipedia.org/wiki/Simonkolleite IntelliBond M - http://webmineral.com/data/Kempite.shtml . 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt The crystal form of each IntelliBond product is slowly solubilized in the abomasum allowing for more efficiently absorption of the metal over the full length of the small intestine improving trace mineral absorption efficacy. The two studies highlighted above indicate the superior bioavailability of IntelliBond products when in a model utilized extensively by several organic trace mineral companies to validate the bio-efficacy of their products. It is interesting that no major organic trace mineral company support their products based on relative bioavailability given the fact that published research demonstrates a potential benefit of 10 to 15%, however their pricing is more in the range of 600 – 700% of the value of typical sulfate forms. IntelliBond trace minerals represent an opportunity to significantly improve trace mineral absorption at a price point that is far less than typical organic trace mineral forms.
The study provides a head to head comparison between IntelliBond C fed at 25 to 100 ppm with two leading organic sources (Availa Copper and BioPlex copper) fed at ISO levels under commercial conditions. You will see that there were no statistically significant differences between the sources at each level of comparsion. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
A study was conducted to evaluate the relative bioavailability of IntelliBond C (tri basic copper chloride) in lambs compared with Copper Lysine. You will see there were no statistically significant difference between sources. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
Study conducted by Dr. J. Arthington, University of FL was designed to compare IntelliBond C directly with a good Organic trace mineral (Availa Cu) in cattle experiencing a heavy copper antagonistic challenge 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
Most nutritionists would start by looking at the use of sulfate based trace minerals within their program (line 1). This is true based on past experience and their cost relative to other trace mineral options. However, most nutritionists are fully aware that there are many negative issues associated with the use of sulfates (poor stability, antagonistic reactions in the feed and the digestive tract, handling, etc.). Given these negatives, “if price” was not an issue and given what we know about better quality organic trace minerals we would move all trace mineral supplementation to organic forms (line 2). However, cost is an issue ($5.16 versus $37.40 per cow per year) between the two programs prompting nutritionists who wish to improve the quality of the trace mineral program compromise by reducing the quality of organic trace mineral in their formulation to a level that will fit with the cost restraints of their clients (line 3). While this program does improve the quality of the program, it still relies heavily on the use of sulfates to meet the trace mineral specification of the cow. Effectively, you are almost tripling the cost of the trace mineral program to replace 25% of the total level of mineral being supplied to the cow. Hydroxy trace minerals provide you with the cost effective ability to replace all of the sulfate and organic trace minerals in the program with IntelliBond C, Z and M, (line 4) thereby providing the cow with a trace mineral program that would be equivalent or better than a total replacement with organic trace mineral but at a price point that is approximately 12% less than the combo (line 3). This is the unique value that only IntelliBond trace minerals can provide, significantly improved bioavailability at a cost point that will reduce trace mineral fed costs in program utilizing an organic trace mineral in combination with sulfates. Line 5 represents a program being utilized by nutritionists as a way to take advantage of IntelliBond’s increased bioavailability over sulfates (given the fact that we are replacing 75% of the sulfate based trace mineral with hydroxy trace minerals you are significantly increasing the level of trace mineral bioavailability in the diet, giving you the option to reduce the amount of hydroxy trace mineral being fed, with no negative impact on cow productivity / well being. Obviously this allow for a further reduction in cost per cow and improvement in environmental compliance. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
Comprehensive transition cow study conducted with Dr. Tom Overton utilizing cows from the Cornell University research herd. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
IntelliBond supplemented cows quickly allowed for an increase in fat corrected milk production above and beyond the combo cows and cows supplemented with just sulfates 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
The table summarizes the actual tabular results of the study for all production parameters. While there were no direct treatment effects, IntelliBond supplemented cows did produce statistically significant increased in milk, fat, lactose and fat corrected milk above and beyond sulfate only and combo cows. It was also observed that cows receiving IntelliBond trace minerals lost less BW than the other two treatments during both the pre and postpartum periods. There were no statistical difference in SCC, locomotion or other health related parameters through out the study. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
IntelliBond treated cattle were more effective at reducing TBAR concentration indicating a lower level of antioxidant activity in the cow, generally indicating a higher cow health status within the cows receiving IntelliBond. This was further confirmed by lower TAC levels in the cow. 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
The above table summarized the economic results of the study based on the NE milk order pricing grid as of 12/31/11. Looking at the comparison of program costs (last row) with incremental value gained from the use of IntelliBond (first row) you will quickly see that the use on IntelliBond produced a significant ROI over either the cows receiving straight sulfate minerals or the cows receiving the combination (75% sulfate / 25% organic). 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt
Given the combination of improved bio efficacy and lower cost, IntelliBond products provide nutrition professionals with the opportunity to finally get rid of all inorganic trace minerals in the ration and higher priced organics, replacing both with a constant source of highly bioavailable race minerals designed to increase trace mineral absorption, thereby driving animal productivity and health at potentially lower levels of trace miner.al inclusion in the diet 1500-19387 02/13/13 Creative\\Clients\\Micronutrients\\1500-19387 IntelliBond Cornerstone PowerPoint\\1500-19387 IntelliBond Cornerstone PowerPoint Presentation (for internal use only)_v31.ppt