CRISPR/Cas9 Platform

Our platform performs gene knockout/knockin
programme with CRISPR/Cas9 system
CRISPR/Cas9 Platform
https://www.creative-biogene.com/crispr-cas9/
info@creative-biogene.com

An Overview of CRISPR/Cas9 Platform
CRISPR-Cas9 is a novel genome-engineering technology in recent years.
Comparing with previous TALEN and ZFN technologies, CRISPR-Cas9 shows
several advantages for genome editing, including simple-to-design, easy-to-use and
multiplexing (capable of editing multiple genes simultaneously). The CRISPR
system consists of two components, RNA fragment and Cas9 protein. Guide RNA
with specific sequences is used to define the genomic target and assure the precise
of this technology, while Cas9 protein cuts the genomes at defined site just like the
god hand.

An Overview of CRISPR/Cas9 Platform
As a leading provider of gene editing service, Creative Biogene has launched a
powerful CRISPR/Cas9 Platform. Our platform performs gene knockout/knockin
programme with CRISPR/Cas9 system, from designing gRNA constructs to
transfection and single clone generation of a wide range of cells, including difficult-
to-transfect and tumor cell lines, as well as plants, mouse and other animal models.

—— SgRNA Design and Confirmation
The key components of CRISPR system are Cas9 nucleases and guide RNA. In term of
targeting different sites genome, sgRNA varies the sequence to each target. A high-
quality sgRNA is the foundation of successful gene-editing process. CRISPR/Cas9
PlatformCB brings customers a set of sgRNA service with high quality, including sgRNA
design, construction and validation. Additionally, off-target analysis is available. We
provide one of these services separately or a combined service depending on your needs.
Before ordering, customers could contact us with your project for free consultation.

—— Custom Knockout Cell Line Generation
Gene knockout cell line is a useful tool for research and manufacture. But it takes a long
time to generate a knockout cell line. The most common method for gene editing is to
use engineered nucleases, which can induce a double-strand break in genomic DNA. As
the latest technology, CRISPR/Cas9 are widely accepted for gene knockout and knockin.
Depending on the excellent platform and experienced scientists, our staff has
succeeded in lots of gene knockout projects, ranging from tumor cell line to difficult-to-
handle cell line.
An example of gene editing by CRISPR/Cas9

—— Custom Knockin Cell Line Generation
CRISPR/Cas9 PlatformCB provides one-stop
custom knock-in cell line generation service,
including point mutation and gene insertion.
Currently, the most commonly used system is
derived from Streptococcus pyogenes (Sp),
which consists of a Cas9 nuclease and a guide
RNA. SpCas9 will induce a DNA double-
stranded break after recruited to specific site by
sgRNA. The subsequent homologous
recombination repair will generate a knock-in or
point mutation phenotype in the presence of a
recombination donor. Thus, the HDR pathway
are exploited to facilitate correction of diseased
genes, insertion of epitope tags or fluorescent
reporters, and overexpression of genes of
interest in a site-specific manner.

—— Genome Editing Models Generation
Genome engineering models are important
tools, which are widely used in life science
research, such as gene function research,
cancer research and drug discovery.
Eukaryotic genomes contain billions of DNA
bases and are difficult to manipulate. To
overcome this barrier, a series of
programmable nuclease-based genome
editing technologies have been developed.
CRISPR/Cas9 PlatformCB utilizes
CRISPR/Cas9 technology to generate
custom genome-editing animal models for
customers. Various species are available,
including mouse, rat, zebrafish, C.elegans.
Work flow

—— Gene Knockout Mouse Generation
CRISPR/Cas9 PlatformCB offers gene knockout mouse generation services for our
clients. At CRISPR/Cas9 PlatformCB, the custom service begins with a free consultation.
After customer submits the detailed requirement, our scientists will show the client
feasible analysis result and an optional strategy to achieve the goal. Once customers
confirm the order, our staff would begin to construct required vectors and prepare zygote
for microinjection. Cas9 and sgRNA are directly injected to zygotes where gene editing
will occur. Mice that develop from these embryos are genotyped or sequenced to
determine if they are correctly engineered. Every steps are well-organized and arranged.

—— Knockin Mouse Generation
The knockin mouse is an important research model in which an exogenous gene or DNA
fragment is introduced into the mouse genome and transmitted to next generation.
Knockin mice are utilized in a wide range of application, from the study of gene function to
the production of therapeutically humanized antibodies. As a latest technology,
CRISPR/Cas9 become a main method for genome editing mice generation.
CRISPR/Cas9 PlatformCB provides a knockin mice generation service, including point
mutation mice, ROSA26 knockin mice, custom knockin mice and conditional knockin mice.

—— Genome Editing in Plants
Genetic mutants are critical for the study of gene functions in plants and for genetic
improvement of crops. Plant biologists study molecular mechanisms to understand plant
function, fight disease and enhance plant productivity. CRISPR/Cas9, as an up-to-date
technology, brings a revolution for plant research as well. CRISPR/Cas9 PlatformCB
offers a one-stop plant genome editing service, including gene knockout, gene knockin
and gene deletion. Various species are available for this service, including but not limited
to Arabidopsis thaliana, Zea mays, rice and tobacco.

—— Microbial Genome Editing Service
As a premier institution in microbial
genome editing, CRISPR/Cas9
PlatformCB has years of experience to
meet your specific project needs in
using the CRISPR/Cas9 technology to
add value to your research project.
CRISPR/Cas9 PlatformCB offer a
series of services for genetic
modification which has been
successfully achieved in
microorganisms, such as E. coli,
Salmonella, Streptomyces, Clostridium,
Bacillus subtilis, cyanobacteria,
Saccharomyces cerevisiae,
filamentous fungi, and so on.

—— Product
CRISPR/Cas9 PlatformCB also offers a broad range of CRISPR/Cas9 products to assist
our clients for gene editing. Moreover, all of products have the best performance in both
research and industry field. Besides the products serving for gene editing, we still provide
pre-made KO cell lines that can be directly used for research or manufacture.

THANK YOU

CRISPR/Cas9 Platform

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