Commando Business Club Special Event 2016

•

1 like•248 views

Roy Schoemaker MCM CMC

Commando Business Club Special Event 2016

DNA sequence variants play an important role in the initiation and progression of many different cancer types. The detection of germline variants at a fixed ratio by gold-standard Sanger sequencing has been well established; however, the detection of somatic mutations, especially in heterogeneous tumor samples where variants may be present at a lower level, has been more challenging. Minor Variant Finder Software (MVF) enables calling of low frequency variants at a detection level as low as 5% using Sanger sequencing. We have developed gene-specific Sanger sequencing panels covering the entire coding region (all exons) of specific genes (e.g., TP53, KRAS, and NRAS) implicated in tumorigenesis. We initially determined variants of TP53 and KRAS from lung tumor FFPE samples by NGS using the Ion PGM™ System. We confirmed the identity and minor allele frequency of these variants by gene-specific Sanger sequencing panels analyzed by MVF. To demonstrate the robustness and flexibility of using Sanger sequencing for oncology research, we also included variants across many different solid tumor types in a pan-cancer panel. We tested this workflow with lower amounts of DNA input (10ng, 3ng, 1ng, 0.1ng). Additionally, we have built an extended RAS panel including eight amplicons covering the most important codons (12-13, 59-61, 117 and 146) of KRAS and NRAS genes. The entire workflow and data analysis using MVF was validated on thirty-five FFPE samples derived from colon cancer biopsies by OmniSeq LLC, Buffalo, NY.

香港六合彩

香港六合彩香港六合彩

下午，香港六合彩很早就收了工，香港六合彩去看守所探视吴军。香港六合彩在街上买了十斤橘子，吴军最喜欢吃橘子。现在香港六合彩能送的只有这橘子了。探监室的门开了，吴军手带手铐走了出来，见到香港六合彩开心的笑了，香港六合彩记得这笑容仿佛是香港六合彩在初一刚开学时见到吴军的笑容，那笑容里包含着青涩。香港六合彩说：香港六合彩带了香港六合彩爱吃的橘子。吴军问：香港六合彩过得好吗？香港六合彩被这一问，泪水涌满眼眶，这句话以前在电话中，吴军不知问候了多少次，香港六合彩的苦，香港六合彩的乐，香港六合彩的思想，吴军与香港六合彩分享的最多，而现在，吴军却因贩毒而枪决，为什么要贩毒！香港六合彩想到这儿，狠狠地说：为什么要贩毒，为什么？难道香港六合彩不知道贩毒是死路一条吗？

Commercial Real Estate: Hot Topics October 2015

CBIZ, Inc.

CBIZ Federal Tax Case Study

CBIZ, Inc.

CBIZ Small Business Employment Index - November 2015

CBIZ, Inc.

Tik bab 2

febriyankusumaazizpradana9e

Study of genetic Information from cell-free (cf) DNA provide valuable opportunities in cancer research and potentially impact future oncology. As an example, liquid biopsy provide a non-invasive and cost effective solution for future compared to traditional biopsy tests. Here we report the application of research based Ion Torrent™ next-generation sequencing (NGS) Oncomine™ cfDNA assays and associated workflow, which is developed to detect somatic variants at low frequency of 0.1% in cfDNA from plasma.